Os07g0203700
The rice Os07g0203700 was reported as SPL5 in 2012 [1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os07g0203700 <=> SPL5,OsSPL5
Function
- SPL5 encodes a putative splicing factor 3b subunit 3 (SF3b3)
- SPL5 is a cell death and defense-related gene in rice
- SPL5 might be involved in splicing reactions of pre-mature RNAs participating in the regulation of cell death and resistance responses.
- SPL5 negatively regulates cell death and resistance responses via modulating RNA splicing in plants.
Phenotypic analysis
- Rice lesion mimic mutant spotted leaf 5 (spl5), induced by a radiation, spontaneously occurs cell death mainly in leaves. Under natural field conditions (25–35°C), the spl5 mutant displayed relatively small reddish brown necrotic lesions on leaves as early as 15–20 days after sowing, and then lesions continuously developed and finally scattered over the whole leaf surface (Fig. 1b, d).
- Even under aseptic conditions, these lesions were still formed on the mutant leaves (Fig. 1e, f). Leaves from the mutant and wild-type Zhefu 802 were respectively stained with trypan blue, a histochemical indicator of irreversible membrane damage or cell death (Dietrich et al. 1994).
- The trypan-blue staining revealed that numerous microscopic lesions consists of clusters of a few dead cells throughout the spl5 leaves, and on the contrary, no necrotic lesion was observed on the wild- type leaves (Fig. 1g, h).
- Further histochemical assays showed that HR-marker compounds, reactive oxygen species (ROS) such as H 2 O 2 and callose were greatly accumulated in the mutant leaves, whereas their accumulation were not observed in the wild-type leaves (Fig. 1i–l).
Figure 1. HR-like lesion mimic phenotypes of the spl5 mutant.[1].
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Labs working on this gene
- College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China
- Institute of Genetic and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
- China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, China
