Os07g0498800
CRTintP1 confers cold tolerance on rice plants[1].
Contents
Annotated Information
Function
CDPK13, calreticulin and CRTintP1 might be important signaling components for response to cold stress in rice[1]. CRTintP1 was identiWed as a calreticulin-interacting protein by yeast two-hybrid system and was binding to calreticulin using immuno-precipitation system, which indicating that CDPK13, calreticulin and CRTintP1 were related with each others[2].
GO assignment(s): GO:0006464
Mutation
Transgenic rice[1]:
- Accumulation of calreticulin protein and CRTintP1 protein in the sense calreticulin and CRTintP1 transgenic rice was higher than those in vector control transgenic rice, respectively.
- Calreticulin protein in sense CRTintP1 transgenic rice and CRTintP1 in sense calreticulin transgenic rice were also higher than that in the vector-control transgenic rice. These data demonstrate the interaction of calreticulin with CRTintP1 in vivo, which is in agreement with the observations obtained from the yeast two-hybrid analysis.
Expression
- Northern and immunoblot analysis showed increased expression of calreticulin and CRTintP1 in response to cold stress. Co-immunoprecipitation using anti-calreticulin antibodies confirmed the existence of the calreticulin- CRTintP1 complex in vivo in the stressed leaf tissue[3].
- CRTintP1 was identified as a calreticulin-interacting protein by yeast two-hybrid systems and was found bound to calreticulin using immono-precipitation system. The interaction of calreticulin with CRTintP1 in vivo, which is in agreement with the observations obtained from the yeast two-hybrid analysis[2][3].
Subcellular localization
Cellular localization studies showed that a fusion of CRTintP1 to green fluorescent protein was directed to the nucleus in onion epidermal cells[2].
Knowledge Extension
- Calreticulin (CRT), a major Ca2+-sequestering protein, has been implicated in a variety of cellular functions such as Ca2+ storage, signaling and chaperone activity within the cytoplasm and endoplasmic reticulum. To investigate the biological role of CRT in rice, 21 partial cDNAs, encoding proteins that interacted with rice CRT in a yeast two-hybrid interaction-cloning system, were characterized and the nucleotide sequences were found to be identical to each other[2].
- Northern and immunoblot analysis showed increased expression of CRT and CRTintP in response to cold stress. Co-immunoprecipitation using anti-CRT antibodies confirmed the existence of the CRT-CRTintP complex in vivo in the stressed leaf tissue, suggesting their potential role in regulating stress response[2].
Labs working on this gene
- National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba 305-8518, Japan
- National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan
- National Agricultural Research Center for Tohoku Region, Akita 014-0102, Japan
References
- ↑ 1.0 1.1 1.2 Komatsu S, Yang G, Khan M, et al. Over-expression of calcium-dependent protein kinase 13 and calreticulin interacting protein 1 confers cold tolerance on rice plants[J]. Molecular Genetics and Genomics, 2007, 277(6): 713-723.
- ↑ 2.0 2.1 2.2 2.3 2.4 Sharma A, Isogai M, Yamamoto T, et al. A novel interaction between calreticulin and ubiquitin-like nuclear protein in rice[J]. Plant and cell physiology, 2004, 45(6): 684-692.
- ↑ 3.0 3.1 Komatsu S. Rice proteome database: a step toward functional analysis of the rice genome[J]. Plant molecular biology, 2005, 59(1): 179-190.
