Os07g0616000

The rice Os07g0616000 was reported as DEP2 and CL7(t) in 2010 and 2014 by researchers from China.

Annotated Information

Figure 1. Phenotype of the dep2 mutant..

Gene Symbol

• Os07g0616000 <=> CL7(t),DEP2,OsDEP2

Function

• DEP2 encodes a plant-specific protein without any known functional domain .
• Morphological and expression analysis indicated that mutation in DEP2 mainly affects the rapid elongation of rachis and primary and secondary branches, but does not impair the initiation or formation of panicle primordia.
• Rice DENSE AND ERECT PANICLE 2 (DEP2) is essential for determining panicle outgrowth and elongation.
• Researchers think that the study of DEP2 not only strengthens our understanding of the molecular genetic basis of panicle architecture but also has important implications for rice breeding.

Phenotypic analysis

• To elucidate the molecular mechanism of panicle erectness, two dense and erect panicle mutant alleles, dep2-1 and dep2-2, derived respectively from Oryza sativa L. ssp. japonica cultivar Zhonghua 11 and Nipponbare were identified [26]. Phenotypic analysis indicated that the morphology of dep2 was comparable with the wild type plant from the vegetative developmental stage to the early reproductive stage.
• However, wild type panicles begin to bend 3 weeks after flowering as grain weight increases, while the panicles of dep2 remained upright, even after the grains were fully matured (Figure 1A).
• Besides panicle erectness, the dep2 mutants also showed a slight reduction in plant height (Figure 1A and Table 1), an obvious decrease in panicle length (Figure 1B), and a significant increase in both rachis and stem diameter (Table 1). The leaves of dep2 are short, wide, and erect, and the overall appearance of the mutant is more compact compared to the wild type.
• Detailed analysis showed that there were no difference in the number of primary and secondary branches (Figure 1D), and the total number of spikelets per panicle between the wild type and the mutant (Table 1). The grain density is increased due to the decreased panicle length but not the change of grain number.

Figure 7. Subcellular localization of DEP2..

• The grains of the mutant are wider and shorter than the wild type (Figure 1C), causing a slight decrease in the 100-grain weight, 2.51 g in dep2-1 in contrast to 2.72 g in Zhonghua11 (Table 1). These results indicate that mutation in DEP2 has pleiotropic effects on plant architecture, and that increased diameter of the rachis and decreased panicle length altogether contributed to the dense and erect panicle phenotype.

Expression

• Expression profiling of DEP2 revealed that it is highly expressed in young tissues, with most abundance in young panicles.

Subcellular localization

• Subcellular localization analysis showed that DEP2 appears to be ubiquitously distributed in plant cells.

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Labs working on this gene

• State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
• Graduate School of the Chinese Academy of Sciences, Beijing 100101, China
• Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, PR China
• Key Laboratory of Ion Beam Bioengineering, Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences, Hefei 230031, PR China
• Key Laboratory of Resource Plant Biology of Anhui Province, College of Life Sciences, Huaibei Normal University, Huaibei 235000, PR China
• Institute of Agricultural Engineering, Anhui Academy of Agricultural Sciences, Hefei 230031, PR China
• College of Life Sciences, Anhui Agricultural University, Hefei 230031, Anhui, PR China