Os08g0160500

The rice Os08g0160500 was reported as cslf6 in 2012 by researchers from USA, Australia and Korea.

Annotated Information

Gene Symbol

• Os08g0160500 <=> CslF6,OsCslF6

Function

• The rice CslF6 gene mediates the biosynthesis of MLG(Mixed-linkage glucan).

Expression

• Rice cslf6 knockout mutants show a slight decrease in height and stem diameter but otherwise grew normally during vegetative development.
• However, cslf6 mutants display a drastic decrease in MLG content (97% reduction in coleoptiles and virtually undetectable in other tissues).
• Immunodetection with an anti-MLG monoclonal antibody revealed that the coleoptiles and leaves retain trace amounts of MLG only in specific cell types such as sclerenchyma fibers.
• These results correlate with the absence of endogenous MLG synthase activity in mutant seedlings and 4-week-old sheaths.
• Mutant cell walls are weaker in mature stems but not seedlings, and more brittle in both stems and seedlings, compared to wild type.
• Mutants also display lesion mimic phenotypes in leaves, which correlates with enhanced defense-related gene expression and enhanced disease resistance.

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Labs working on this gene

• Joint BioEnergy Institute, Emeryville, California 94608;
• Divisions of Physical Biosciences and Life Sciences,Lawrence Berkeley National Laboratory, Berkeley, California 94720;
• Commonwealth Scientific and Industrial Research Organization Food Futures Flagship and Commonwealth Scientific and Industrial Research Organization Plant Industry, Black Mountain Laboratories, Black Mountain, Australian Capital Territory 2601, Australia;
• Department of Plant Pathology and The Genome Center, University of California, Davis, California 95616
• Biomass Science and Conversion Technologies Department, Sandia National Laboratories, Livermore, California 94551;
• Department of Plant Molecular System Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446–701, Korea