Os08g0187500
The rice Os08g0187500 was reported as ROC1 in 2016 [1] by researchers from China.
Contents
Annotated Information
Function
- ROC1 is a positive regulator of cold stress responses.
Phenotypic analysis
- To detect the role of ROC1 in response to chilling stress, a ROC1 T-DNA mutant and the corresponding wild-type plants were compared. In this mutant, T-DNA was located in the second intron of ROC1 gene.
- In normal growth conditions in the growth chamber, no visible phenotypes were observed between roc1 mutants and wild-type plants (Figure 4c). Next, we tested chilling stress responses in wild-type and roc1 mutants. Fifteen-day-old plants were transferred to a low temperature chamber (4 °C) for four days and moved back to the normal growth chamber (28 °C) to determine the survival rates.After 10 days of recovery, ~94% of wild-type plants were recovered, while ~16% of roc1 mutants survived.
Expression
- The ROC1 expression pattern was examined in roots, leaves, shoot apices and flowers tissues. ROC1 mRNA was expressed in all tissues and was found to be highly expressed in shoot apices.
- The expression levels of ROC1 in four T2 transgenic plants were analyzed by qRT-PCR. The results showed that ROC1 was suppressed at different levels and that the Ri5 plant showed strong suppression.
- To verify the phenotypic expression of roc1 mutants in response to chilling stress, the survival rate was analyzed from Ri3 and Ri5 transgenic lines.
Subcellular localization
- To understand the subcellular localization of ROC1, ROC1-GFP was expressed in onion epidermis cells under the control of a non-specific promoter (35S). Free GFP expression was observed in the cytosol and nucleus, while ROC1-GFP was detected in the nucleus.
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Labs working on this gene
- State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
- Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
- State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
- State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
- State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
References
- ↑ Xu Y, Zhang S, Guo H, Wang S, Xu L, Li C, Qian Q, Chen F, Geisler M, Qi Y, Jiang de A. OsABCB14 functions in auxin transport and iron homeostasis in rice (Oryza sativa L.). Plant J. 2014 Jul;79(1):106-17. doi: 10.1111/tpj.12544. Epub 2014 Jun 13. PubMed PMID: 24798203.
