Os08g0191700

The rice Os08g0191700 was reported as OsGLYI-11.2 in 2014 by researchers from India.

Annotated Information

Figure 5. Germination and growth assay of OsGLYI-11.2 transgenic tobacco under various abiotic stress conditions..

Gene Symbol

• Os08g0191700 <=> OsGLYI-11.2,OsGLYI11,GLYI11

Function

• OsGLYI-11.2 requires Ni2+ for its activity. Its biochemical, structural and functional characterization revealed it to be a monomeric enzyme, possessing a single Ni 2+ coordination site despite containing two GLY I domains.

Phenotypic analysis

• Heterologous expression of OsGLYI-11.2 in Escherichia coli and model plant Nicotiana tabacum (tobacco) resulted in improved adaptation to various abiotic stresses caused by increased scavenging of MG, lower Na + /K + ratio and maintenance of reduced glutathione levels.
• Seeds from WT and OsGLYI-11.2 transgenic plants (line 7.1) showed comparable germination under non-stress conditions (Figure 5a, panel 1); however, under osmotic, MG, oxidative and salinity stress, the germination and growth rate of WT reduced significantly when compared with the transgenic line (Figure 5a, panels 2–4).
• Under stress conditions, the shoot and the root lengths of the WT were severely affected, whereas the transgenic plants showed healthier leaves and better root growth (Figure 5b,c). Furthermore, to score visual stress-induced injury, we calculated the stress tolerance index (STI%) based on their ability to grow under different stress conditions as compared with the non-stressed condition.
• STI was measured in terms of total fresh weight of both shoots and roots. The negative effect on growth was much higher in WT seedlings as compared with the OsGLYI-11.2 transgenic seedlings under all the stresses in terms of STI (Figure 5d).

Expression

• The expression of OsGLYI-11.2 was found to be highly substrate inducible, suggesting an important mode of regulation for its cellular levels.

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Labs working on this gene

• Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
• Structural and Computational Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
• School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India
• Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India