Os08g0430500

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The rice gene Os08g0430500 was reported as GF14c in 2009[1] by researchers from Japan.

Annotated Information

Gene Symbol

  • Os08g0430500 <=> GF14c, OsGF14c

Function

  • GF14c encodes an Hd3a-interacting protein.
  • GF14c acts as a negative regulator of flowering by interacting with Hd3a.

Mutation

Fig. 5 Characterization of gf14c T-DNA insertion plants. [1].
  • To study further the function of GF14c in fl owering, we also used the gf14c/GF14c heterozygous T-DNA insertion mutants in japonica rice cv ‘Dongjin’ generated by Postech, Korea. The gf14c/ GF14c plants fl owered earlier than those of the wild type by about 14 d (12–17-d depending on the plants)
  • GF14c-overexpression plants were delayed by 5–20 d in fl owering relative to wild-type plants

Expression

Evolution

Fig. 2 Tissue-specific GF14s family members in rice. [1].
  • GF14s belong to the 14-3-3 protein group and have eight isoforms, designated as GF14a–h in rice [2][3], that share 85–95% amino acid identity ( Fig. 2A ). To determine whether the GF14 genes are expressed in rice organs, we performed semi-quantitative reverse transcription–PCR (RT–PCR) using specifi c primers ( Fig. 2B ).

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Labs working on this gene

  • Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0101 Japan

References

  1. 1.0 1.1 1.2 Purwestri YA, Ogaki Y, Tamaki S, Tsuji H, Shimamoto K. The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a. Plant Cell Physiol. 2009 Mar;50(3):429-38. doi: 10.1093/pcp/pcp012. PubMed PMID: 19179350.
  2. Chen , F. , Li , Q. , Sun , L. and He , Z. ( 2006a ) The rice 14-3-3 gene family and its involvement in responses to biotic and abiotic stress. DNA Res. 13 : 53 – 63 .
  3. Yao , Y. , Du , Y. , Jiang , L. and Liu , J. ( 2007 ) Molecular analysis and expression pattern of the 14-3-3 gene family. J. Biochem. Mol. Biol. 40 : 349 – 357.


Structured Information