Os08g0445700
The rice Os08g0445700 was reported as OsTPS1 in 2011 [1] by researchers from China.
Contents
Annotated Information
Function
- Trehalose-6-phosphate synthase (TPS) is a key enzyme for trehalose biosynthesis.
- Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice
- Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms.
- In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). The genome of rice (Oryza sativa) contains 11 OsTPS genes, and only OsTPS1 shows TPS activity.
Phenotypic analysis
- Leaves of OsTPS1 knockout (KO) plants were more sensitive to drought or cold stress than were the wild types.
- Furthermore, transgenic rice of UBI::CBF1 had high expression OsTPS1 mRNA, suggesting that OsTPS1 is regulated by the CBF/DREB transcription factor.
- OsTPS1 overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant phenotypic changes
Expression
- Reverse transcriptase-PCR analysis of OsTPS1 showed that OsTPS1 is inducible by drought, salt, cold, and ABA.
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Labs working on this gene
- Key Laboratory of Gene Engineering Drug and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, 100875, People's Republic of China.
References
- ↑ Li HW, Zang BS, Deng XW, Wang XP. Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice. Planta. 2011 Nov;234(5):1007-18. doi: 10.1007/s00425-011-1458-0. PubMed PMID: 21698458.