Os08g0445700

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The rice Os08g0445700 was reported as OsTPS1 in 2011 [1] by researchers from China.

Annotated Information

Function

  • Trehalose-6-phosphate synthase (TPS) is a key enzyme for trehalose biosynthesis.
  • Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice
  • Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms.
  • In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). The genome of rice (Oryza sativa) contains 11 OsTPS genes, and only OsTPS1 shows TPS activity.

Phenotypic analysis

  • Leaves of OsTPS1 knockout (KO) plants were more sensitive to drought or cold stress than were the wild types.
  • Furthermore, transgenic rice of UBI::CBF1 had high expression OsTPS1 mRNA, suggesting that OsTPS1 is regulated by the CBF/DREB transcription factor.
  • OsTPS1 overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant phenotypic changes

Expression

  • Reverse transcriptase-PCR analysis of OsTPS1 showed that OsTPS1 is inducible by drought, salt, cold, and ABA.

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Labs working on this gene

  • Key Laboratory of Gene Engineering Drug and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, 100875, People's Republic of China.

References

  1. Li HW, Zang BS, Deng XW, Wang XP. Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice. Planta. 2011 Nov;234(5):1007-18. doi: 10.1007/s00425-011-1458-0. PubMed PMID: 21698458.

Structured Information