Os09g0469400
The rice gene Os09g0469400 was reported as isa3 in 2011[1] by researchers from Japan.
Contents
Annotated Information
Gene Symbol
- Os09g0469400 <=> OsISA3,ISA3
Function
- ISA3 facilitates starch metabolism and affects morphological characteristics of plastids in rice.
Mutation
- A transposon-inserted rice mutant of isoamylase3 (isa3) contained an increased amount of starch in the leaf blade at the end of the night, indicating that ISA3 plays a role in the degradation of transitory starch during the night.
- Furthermore, chloroplasts in the leaf blade of isa3 seedlings were large and pleomorphic.
Overexpression
- Overexpression of ISA3 in the sugary1 mutant, which is deficient in ISA1 activity, did not convert water-soluble phytoglycogen to starch granules, indicating that ISA1 and ISA3 are not functionally redundant.
Expression
- ISA3–green fluorescent protein (GFP) fusion protein expressed under the control of the rice ISA3 promoter was targeted to the amyloplast stroma in the endosperm.
Fig. 3 ISA3–GFP is localized in the amyloplast stroma in the rice endosperm. [1].
Evolution
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Labs working on this gene
- Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602 Japan
- Rice Quality Research Team, National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, 305-8518, Japan
- Present address: Food Resource Division, National Food Research Institute, 2-1-12 Kannondai, Tsukuba, 305-8642 Japan
- Present address: National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira, Sapporo, 062-8555 Japan
References
