Os09g0563300

The rice Os09g0563300 was reported as TCD9 in 2014 by researchers from China.

Annotated Information

Figure 6. Subcellular localization of pMON530-SPTCD9-GFP protein..

Gene Symbol

• Os09g0563300 <=> OsTCD9,TCD9

Function

• TCD9 encoding α-subunit of Cpn60 protein (Cpn60α),
• TCD9 plays a crucial role in chloroplast development at early leaf stage of rice.

Phenotypic analysis

• The leaves of tcd9 mutant appeared albino phenotype before the 3-leaf stage when grown below 24 ◦C, but the 4th leaves gradu- ally turned green and appeared normal green after the 5-leaf stage even at 20 ◦ C (Fig. 1A).
• The color and height of tcd9 mutant was no discrepancy with wild type when grown beyond 28 ◦C (Fig. 1A). Similarly, the contents of Chl a, Chl b, total Chl and carotenoid (Car) in tcd9 plants before the 3-leaf stage at 20 ◦C were drastically lower than those in the wild type plants and itself grown beyond 28 ◦C(Suppl. Table S3); however, the contents were comparable between wild type plants and tcd9 plant grown at 32 ◦ C (Fig. 1B).
• The observations suggested the thermo-sensitivity of albino phenotype in the mutant as 7436S, Fan5, v1, v2 and v3

Expression

• qRT-PCR analysis showed that TCD9 exhibited the high expression in all tested tissues,especially in young leaves.

Subcellular localization

• Subcellular localization analysis showed that the GFP fluorescence was colocalized with chlorophyll autofluorescence, showing that TCD9 was a chloroplast-localized protein.

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Labs working on this gene

• Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China
• The Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 12 South Zhong-Guan Cun Street, Beijing 100081, China
• Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China