Os10g0558900

The rice Os10g0558900 was reported as RL14 in 2012 by researchers from China.

Annotated Information

Figure 2. Phenotypic analysis of RL14 mutants

Gene Symbol

• Os10g0558900 <=> RL14,OsRL14

Function

• RL14 encode a 2OG-Fe (II) oxygenase of unknown function.
• RL14 gene affects water transport in leaves by affecting the composition of the secondary cell wall.
• This change in water transport results in water deficiency, which is the major reason for the abnormal shape of the bulliform cells.

Phenotypic analysis

• rl14 mutant plants had incurved leaves because of the shrinkage of bulliform cells on the adaxial side.
• In addition, rl14 mutant plants displayed smaller stomatal complexes and decreased transpiration rates, as compared with the wild type. Defective development could be rescued functionally by the expression of wild-type RL14.

Expression

• RL14 was transcribed in sclerenchymatous cells in leaves that remained wrapped inside the sheath.
• In mature leaves, RL14 accumulated mainly in the mesophyll cells that surround the vasculature.
• Expression of genes related to secondary cell wall formation was affected in rl14-1 mutants, and cellulose and lignin content were altered in rl14-1 leaves.

Evolution

• The RL14 gene was mapped first to an interval between the simple sequence repeat (SSR) markers RM3123 and RM1162 on the long arm of chromosome 10.
• Finally, the RL14 locus was mapped to a 24.5-kb DNA region between the InDel marker SID2 and SSR marker SW24 on a single bacterial artificial chromosome, AC079874.
• RL14 was related most closely to members of the 2OG-Fe (II) oxygenase family from rice and sorghum.
• Orthologous of RL14 was identified in other species, such as maize, Arabidopsis, Papaver somniferum, Vitis spp and Ricinus communis.

Labs working on this gene

• Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing, China