Os12g0132400

The rice gene Os12g0132400 was reported as RIP1 in 20 by researchers from Korea.

Annotated Information

Gene Symbol

• Os12g0132400 <=> OsWD40-193, RIP1

Function

• RIP1 is necessary for pollen maturation and germination.
• RIP1 gene encodes a protein that shares significant homology with a group of proteins containing five WD40 repeat sequences.
• RIP1 is probably a nuclear protein that may form a functional complex with other proteins and carry out essential cellular and developmental roles during the late stage of pollen formation.

Mutation

• Microscopic analysis demonstrated that development in the rip1 pollen was delayed, starting at the early vacuolated stage. Close examination of that pollen by transmission electron microscopy also showed delayed formation of starch granules and the intine layer.

• In addition, development of the mitochondria, Golgi apparatus, lipid bodies, plastids and endoplasmic reticulum was deferred in the mutant pollen.

• Under in vitro conditions, germination of this mutant pollen did not occur, whereas the rate for wildtype pollen was 490%.

Expression

• Semi-quantitative reverse transcription–PCR (RT–PCR) analyses confirmed that the RIP1 transcript was abundant at the late stages of pollen development.

Sublocalization

• The green fluorescent protein (GFP)–RIP1 fusion protein is localized to the nucleus.

You can also add sub-section(s) at will.

Labs working on this gene

• National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Republic of Korea
• Functional Genomic Center, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea
• Yeongnam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration, Milyang, 627-130,

Republic of Korea