Os12g0277500

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Annotated Information

Function

• OsCpn60α1 encodes Plastid Chaperonin 60α Subunit.
• OsCpn60α1 is essential for folding of rbcL.
• OsCpn60α1 is required for the folding of rbcL and that failure of that process is seedling-lethal.

Phenotypic analysis

• Homozygous mutant seedlings were pale green (Figs. 3C and 3D). In progeny of the OsCpn60α1/oscpn60α1 heterozygote, that phenotype was segregated in an approximately 3:1 ratio (WT:oscpn60α1); all pale-green seedlings were oscpn60α1 homozygous plants. This indicated that the mutant phenotype was recessive and was caused by a defect in a single genomic locus.
• When leaf chlorophyll contents were measured, the researchers found that both chlorophyll a and b were severely decreased in the mutant, to approximately 37% of that recorded from the WT siblings (Fig. 3E).
• That is, after the fourth leaf emerged from an oscpn60α1 plant, no further leaves were produced, resulting in a lethal character. TEM analyses showed that chloroplast development was impaired in the mutant (Fig.3F).

Figure 3. Phenotypes of oscpn60α1 mutant..

Subcellular localization

• Subcellular localization analysis showed that transiently expressed OsCpn60α1:GFP fusion protein was localized to the chloroplast stroma.

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Labs working on this gene

• Crop Biotech Institute and Department of Genetic Engineering, Kyung Hee University, Yongin 446-701, Korea