Os12g0555600

The rice Os12g0555600 was reported as CSLD4 in 2013 by researchers from Japan.

Annotated Information

Gene Symbol

• Os12g0555600 <=> CD1, CSLD4, DNL3, OsCD1, OsCSLD4

Fig.1.Vegetative phenotypes of wild-type and mutation

Function

• Cellulose synthase-like (CSL) genes are predicted to catalyse the biosynthesis of non-cellulosic polysaccharides such as the β-d-glycan backbone of hemicelluloses and are classified into nine subfamilies (CSLA–CSLH and CSLJ).
• The CSLD subfamily is conserved in all land plants, and among the nine CSL subfamilies, it shows the highest sequence similarity to the cellulose synthase genes, suggesting that it plays fundamental roles in plant development.

Phenotypic analysis

• The mutants of csld4 showed rolled and narrow leaf blades and a reduction in plant height（figure1）.
• The narrow leaf blade of sle1 was caused by reduced cell proliferation beginning at the P3 primordial stage （figure 2）.
• In addition to the size reduction of organs, sle1 mutants exhibited serious developmental defects in pollen formation , anther dehiscence（figure 3）, stomata formation, and cell arrangement in various tissues.

Expression

• In situ hybridization experiments showed that OsCSLD4 was expressed in a patchy pattern in developing organs（figure 4）. Double-target in situ hybridization and quantitative RT-PCR analyses revealed that SLE1 was expressed specifically during the M-phase of the cell cycle（figure 5）, and suggested that the cell-cycle regulation was altered in sle1 mutants. These results suggest that the OsCSLD4 protein plays a pivotal role in the M phase to regulate cell proliferation.

Labs working on this gene

• Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113–8657, Japan
• National Institute of Genetics, 1111 Yata, Mishima, Shizuoka, 411–8540, Japan

References

1. Takanori Yoshikawa;Mitsugu Eiguchi;Ken-Ichiro Hibara;Jun-Ichi Ito;Yasuo Nagato, Rice SLENDER LEAF 1 gene encodes cellulose synthase-like D4 and is specifically expressed in M-phase cells to regulate cell proliferation. Journal of Experimental Botany, 2013, 64(7): 2049-2061

2. Jiang Hu;Li Zhu;Dali Zeng;Zhenyu Gao;Longbiao Guo;Yunxia Fang;Guangheng Zhang;Guojun Dong;Meixian Yan;Jian Liu;Qian Qian.. Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice. Plant Molecular Biology, 2010, 73(3): 283-292

3. Chao Wu;Yaping Fu;Guocheng Hu;Huamin Si;Shihua Cheng;Wenzhen Liu. Isolation and characterization of a rice mutant with narrow and rolled leaves. Planta, 2010, 232(2): 313-324