File list
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| Date | Name | Thumbnail | Size | Description | Versions |
|---|---|---|---|---|---|
| 14:59, 22 May 2014 | 1.pdf (file) |  |
993 KB | 1 | |
| 13:57, 22 May 2014 | F2C.png (file) |  |
44 KB | (C) Phylogenetic analysis revealed that OsRad21-4 was grouped with Rec8-like proteins from other species and formed a clade with Arabidopsis Syn1. The tree was derived from the multiple alignments of N-terminal regions of OsRad21-4 and other Rad21/Rec8 se | 1 |
| 13:38, 22 May 2014 | F10.png (file) |  |
647 KB | Figure 10. FISH of the centromere probe CentO in wild type (A, B) and OsRad21-4 deficient (C–F) plant male meiocytes. CentO was labeled with digoxigenin-labeled dUTP and detected with rhodamin-conjugated anti-digoxigenin antibody (red). Chromosomes wer | 1 |
| 13:37, 22 May 2014 | F9.png (file) |  |
617 KB | Figure 9. Fluorescence in situ hybridization (FISH) using a probe of 25S rDNA in wild type (A, B) and OsRad21-4-deficient (C–F) plant male meiocytes. The probe was labeled with digoxigenin-labeled dUTP and detected with rhodamin-conjugated anti-digoxig | 1 |
| 13:28, 22 May 2014 | F8.png (file) |  |
533 KB | Figure 8. Abnormal meiotic chromosome behavior in microsporocytes of OsRad21-4-deficient lines. Meiosis chromosome spreads were prepared from fixed anthers from the deficient lines (R1 and R5, data are shown only for R5). (A) leptonema; chromatins were | 1 |
| 13:27, 22 May 2014 | F7.png (file) |  |
629 KB | Figure 7. DAPI-stained chromosome spreads of male meiocytes in wild type. (A) leptonema; (B) zygonema; (C) pachynema; (D) diplonema; (E) late diakinesis; (F) metaphase I; (G) anaphase I; (H) dyad; (I) prophase II; (J) metaphase II; (K) anaphase II; (L) te | 1 |
| 13:07, 22 May 2014 | F4.png (file) |  |
831 KB | Figure 4. In situ hybridization analyses of OsRad21-4 mRNA in flowers. Traverse sections of flowers in premeiotic PMC stage (A and B), meiotic PMC stage (C). or uninucleate pollen stage (D) were hybridized with DIG-labeled antisense RNA (A, C and D) or | 1 |
| 13:04, 22 May 2014 | F6.png (file) |  |
413 KB | Figure 6. Pollen viability of OsRad21-4-deficient lines was severely affected. Intact anthers in wild type (A) and deficient lines (B) were stained with TTC solution and spread gently, showing that the deficient-line anthers contained a decreased amoun | 1 |
| 15:34, 20 May 2014 | F4.jpg (file) |  |
107 KB | Figure 4. In situ hybridization analyses of OsRad21-4 mRNA in flowers. Traverse sections of flowers in premeiotic PMC stage (A and B), meiotic PMC stage (C). or uninucleate pollen stage (D) were hybridized with DIG-labeled antisense RNA (A, C and D) or | 1 |
| 15:25, 20 May 2014 | F3C.png (file) |  |
44 KB | Figure3(C) Developmental regulation of OsRad21-4 expression in flowers, which were in carpel and stamen primordial formation stage (F1), premeiotic pollen mother cell (PMC) stage (F2), meiotic PMC stage (F3) or uninucleate microspore stage (F4), and matu | 1 |
| 15:24, 20 May 2014 | F3A.png (file) |  |
28 KB | Figure 3. Accumulation patterns of OsRad21-4 mRNA and protein in different organs. (A) RT-PCR analyses of OsRad21-4 mRNA accumulation. PCR was performed with first-strand cDNAs synthesized with total RNA from flowers (F), leaves (L), buds. | 1 |
| 14:58, 20 May 2014 | F23.png (file) |  |
236 KB | Figure 2(B).OsRad21-4 is a nucleus-localizing protein. Onion epidermal cells were infected by Agrobacterium tumefaciens containing the plasmid harboring a GFP coding sequence (A and B) or OsRad21-4::GFP fusion construct (C and D), These infected cells wer | 1 |
| 09:20, 20 May 2014 | F22.png (file) |  |
145 KB | Figure 2（A）.OsRad21-4 encodes an orthologue of yeast Rec8. OsRad21-4 protein contains the domains and motifs essential to function of Rad21/Rec8 proteins. Pfam04825 and Pfam04824 are shown with single and double underlines, respectively. The potential | 1 |
