Difference between revisions of "Os10g0558900"
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===Expression=== | ===Expression=== | ||
Please input expression information here. | Please input expression information here. | ||
| − | Histochemistry, as well as qRT-PCR analysis, showed that RL14 was highly transcribed in leaves, leaf sheaths and roots. It was also detected in stems and glumes, although its expression in these organs was not as intense as that in the mature leaves, leaf sheaths and roots (Figure | + | Histochemistry, as well as qRT-PCR analysis, showed that RL14 was highly transcribed in leaves, leaf sheaths and roots. It was also detected in stems and glumes, although its expression in these organs was not as intense as that in the mature leaves, leaf sheaths and roots (Figure 1b). |
| − | Expression of RL14 mRNA was detected in the sclerenchymal cell layer of underemerged leaves and sheaths. In the mature leaf, RL14 mRNA accumulated mainly in the mesophyll cells that surrounded the vasculature (Figure | + | Expression of RL14 mRNA was detected in the sclerenchymal cell layer of underemerged leaves and sheaths. In the mature leaf, RL14 mRNA accumulated mainly in the mesophyll cells that surrounded the vasculature (Figure 1c). |
[[File: Analysis of the expression pattern for the RL14 gene.png]] | [[File: Analysis of the expression pattern for the RL14 gene.png]] | ||
| + | Analysis of the expression pattern for the RL14 gene | ||
===Evolution=== | ===Evolution=== | ||
Revision as of 12:56, 9 June 2014
Please input one-sentence summary here.
Contents
Annotated Information
Function
Please input function information here. RL14 (rolling leaf 14) regulates leaf rolling, which is found to encode a 2OG-Fe (II) oxygenase of unknown function. the RL14 gene affects water transport in leaves by affecting the composition of the secondary cell wall. This change in water transport results in water deficiency, which is the major reason for the abnormal shape of the bulliform cells. RL14 mutant plants displayed smaller stomatal complexes and decreased transpiration rates, as compared with the wild type. Defective development could be rescued functionally by the expression of wild-type RL14.
Expression
Please input expression information here.
Histochemistry, as well as qRT-PCR analysis, showed that RL14 was highly transcribed in leaves, leaf sheaths and roots. It was also detected in stems and glumes, although its expression in these organs was not as intense as that in the mature leaves, leaf sheaths and roots (Figure 1b).
Expression of RL14 mRNA was detected in the sclerenchymal cell layer of underemerged leaves and sheaths. In the mature leaf, RL14 mRNA accumulated mainly in the mesophyll cells that surrounded the vasculature (Figure 1c).
Analysis of the expression pattern for the RL14 gene
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this gene
Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing, China.
References
Please input cited references here.
Structured Information
| Gene Name |
Os10g0558900 |
|---|---|
| Description |
2OG-Fe(II) oxygenase domain containing protein |
| Version |
NM_001071899.1 GI:115483393 GeneID:4349365 |
| Length |
2021 bp |
| Definition |
Oryza sativa Japonica Group Os10g0558900, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 10:22450841..22452861 |
| Sequence Coding Region |
22451155..22451400,22452093..22452404 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008403:22450841..22452861 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008403:22450841..22452861 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>tgcatactcatcagagacagaaaatattggactctgcctgctgcaattcatgccaaggccgttggtgttgagccgaagtcgttgttaagtgtatttgaagggcaagctaggggtttgaggatgaactactatccaccgtgcttgaaagctgacaaggtgttgggtttgtcaccgcacactgatccaggtggcttgacactgcttctccaggtgaatgatgtacagggcctgcagatcaacaaggatggcaaatggttctccgtgaatgctctgaatggtgcactcatcgttaacattggcgacacacttgagatcctgagtaatggaaagttcagaagtgttgagcacagggccgtggtacatccaagcagagagaggatttcagcggcactgttctactatccgtgccaggatatggtgatcagccctctgccggactttgtgaaagatggtaaagtgaaatacaaaacaataagctaccaggatttgctgacagagtacttcacagcagagcttgatggaaggaaccggctaaagaaaatgaagctggaaccgtag</cdnaseq> |
| Protein Sequence |
<aaseq>CILIRDRKYWTLPAAIHAKAVGVEPKSLLSVFEGQARGLRMNYY PPCLKADKVLGLSPHTDPGGLTLLLQVNDVQGLQINKDGKWFSVNALNGALIVNIGDT LEILSNGKFRSVEHRAVVHPSRERISAALFYYPCQDMVISPLPDFVKDGKVKYKTISY QDLLTEYFTAELDGRNRLKKMKLEP</aaseq> |
| Gene Sequence |
<dnaseqindica>1462..1707#458..769#atgtactgacagtagctctagctcagtttgagatgtgcaaatagtataacaaaagcattacttatgcaacaaaaactacagctcatcaaccatggagtgccagatgaagtgattgcaaatctcaagaacgacttggttgagtttttcagtcagccactggatgccaagaaggaatactcacagctgcccaataaccttgaaggttatggacaggccttcgttgtgtccgacaaccagaaactagactgggcagacatgctataccttcaagtctgtcctaccgattcaagggacttgagattctggcccaattaccctgcctctttcaggtgatactaccgacaaattattatgcaactaattactccattgataagcatatgttcattataacttttaaagcatacatatgtccatttattaacttgaaatatctgaaaattaggcattccattgatgcatactcatcagagacagaaaatattggactctgcctgctgcaattcatgccaaggccgttggtgttgagccgaagtcgttgttaagtgtatttgaagggcaagctaggggtttgaggatgaactactatccaccgtgcttgaaagctgacaaggtgttgggtttgtcaccgcacactgatccaggtggcttgacactgcttctccaggtgaatgatgtacagggcctgcagatcaacaaggatggcaaatggttctccgtgaatgctctgaatggtgcactcatcgttaacattggcgacacacttgaggtaatgactaatcccgttctcggggcaattgtatcgtatcgatgtaatttcatgagttaaataaagcttttatctttatactattataaaagttgaagatatttttcccggtattttggtgcgtcatccatgtatgagtcagtttctaagttcgttcgcttttggaaatacatatccgtatttgagtcggtttttaagatagttcacttttgataatacagaaggaataatataagaaatctgtttaaaaaactcgcatgctaacatgaggtgatcggactcctaactgcagctcatgattttctaaatatatatatatatatatatatatatatatatatatccaaacgaactcccacaataaatttcatcttaactaaaccatataacaataataatattaaaatagactccaccagttgcaacacacgagcattttttctagttatttaaaaaaaaagaactaatcccgtttgatataggatattaggcagattattttattgtgaatcaaaatatttttaagaaataaatttaacaaatagcttaaaacaaatgatcagtacaaaatcattttggttgtggccgataagccaatgagtagacaatcaaagccgctgatgctcaacttttggatcaacatgtttttctgatgtacaagtacggtcgatgtgcgctgttgatgtgtgcagatcctgagtaatggaaagttcagaagtgttgagcacagggccgtggtacatccaagcagagagaggatttcagcggcactgttctactatccgtgccaggatatggtgatcagccctctgccggactttgtgaaagatggtaaagtgaaatacaaaacaataagctaccaggatttgctgacagagtacttcacagcagagcttgatggaaggaaccggctaaagaaaatgaagctggaaccgtagccatcgaccatgatgcatgtaacaattttataagaagaacctgctttcaggttgtgtcctacttactgtatctgctatctactctcttcatcctaaaataaataaacatagtacatatatgatacatcatagtaccataaattagaaaatgttacatcgtgtactatgtttttttaaaaaatttgggatataggaaatatatgtcgtgaagaataatgtattcaggcatcaagctttatgcataattagcttatgcctgtaaactgcgttgaacatgttcagtttgatgaaacagacatggaagtttgcctctt</dnaseqindica> |
| External Link(s) |
