Difference between revisions of "DEP2"
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DEP2 may be a plant-specific protein. The only existing low similarity to CIP7 in Arabidopsis is found at the N terminal part of the protein, while the COP1-interacting motif, transcription activation domain, and the nuclear localization signal domain are all missing in DEP2. | DEP2 may be a plant-specific protein. The only existing low similarity to CIP7 in Arabidopsis is found at the N terminal part of the protein, while the COP1-interacting motif, transcription activation domain, and the nuclear localization signal domain are all missing in DEP2. | ||
===Knowledge extension=== | ===Knowledge extension=== | ||
| + | *Except dep2,cl7(t) was a novel allele of dep2. Sequence analysis showed that cl7(t) had a single nucleotide substitution (C to A) in the third exon that leads to a Ser substitution with a stop codon, giving a truncated DEP2 protein. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
Revision as of 14:58, 9 June 2014
Contents
Annotated Information
Function
DEP2 gene reduced the development in the longitudinal direction but enhanced the development in the lateral direction of plural organs in rice. In the DEP2 mutants,the panicle length was reduced and panicle diameter was increased[1]. Besides panicle erectness, the DEP2 mutants also showed a slight reduction in plant height , an obvious decrease in panicle length, and a significant increase in both rachis and stem diameter. The leaves of DEP2 are short, wide, and erect, and the overall appearance of the mutant is more compact. The grain density is increased due to the decreased panicle length but not the change of grain number. The grains of the DEP2 mutant are wider and shorter than the wild type[1]. The mutation in DEP2 has pleiotropic effects on plant architecture,and that increased diameter of the rachis and decreased panicle length altogether contributed to the dense and erect panicle phenotype[1]. The DEP2 gene may regulate GA synthesis, and mutation of DEP2 leads to a decrease in plant height[1].
Mutation
The DEP2 locus was mapped to the long arm of rice chromosome 7[1]. dep2-1:The 31-bp deletion in dep2-1 starts at 2184bp from the initiation codon ATG and caused a frameshift; dep2-2:the G/A substitution in the second intron of dep2-2 caused an altered splicing site of the second intron and also led to a frameshift. LOC_Os07g42410 was considered a candidate for the DEP2 gene[1].
Expression
DEP2 gene expression in the dividing zones of rachis, branches, and florets was higher than in other parts of the rice plant. This gene is highly expressed in young panicles ranging from 1 to 15 cm in length;low level expression was also detected in the other organs, including roots,stems,leaves and leaf sheathes.
Evolution
DEP2 may be a plant-specific protein. The only existing low similarity to CIP7 in Arabidopsis is found at the N terminal part of the protein, while the COP1-interacting motif, transcription activation domain, and the nuclear localization signal domain are all missing in DEP2.
Knowledge extension
- Except dep2,cl7(t) was a novel allele of dep2. Sequence analysis showed that cl7(t) had a single nucleotide substitution (C to A) in the third exon that leads to a Ser substitution with a stop codon, giving a truncated DEP2 protein.
Labs working on this gene
Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, PR China Key Laboratory of Ion Beam Bioengineering, Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences,Hefei 230031, PR China Key Laboratory of Resource Plant Biology of Anhui Province, College of Life Sciences, Huaibei Normal University, Huaibei 235000,PR China Institute of Agricultural Engineering, Anhui Academy of Agricultural Sciences, Hefei 230031, PR China College of Life Sciences, Anhui Agricultural University, Hefei 230031, Anhui, PR China State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China Graduate School of the Chinese Academy of Sciences, Beijing 100101, China Department of Bioscience,Fukui Prefectural University,4-1-1 Matsuoka Kenjyojima,Eiheiji-cho,Yoshida-gun,Fukui 910-1195,Japan Institute of Society for Techno-innovation of Agricuture,Forestry and Fisheries.Tsukuba,Ibaraki 305-0854,Japan QTL Genomics Research Center,National Institute of Agrobiological Sciences,Tsukuba,Ibaraki 305-8602,Japan Bioscience and Biotechnology Center,Nagoya University,Chikusa,Nagoya 464-8604,Japan
References
[1]Feng Li,Wenbo Liu, Jiuyou Tang, Jinfeng Chen,et al.(2010)Rice DENSE AND ERECT PANICLE 2 is essential for
determining panicle outgrowth and elongation.Cell Research 20:838-849.
