Difference between revisions of "Os01g0734466"
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===Expression=== | ===Expression=== | ||
*Real-time RT-PCR using specific primer sets for OsrbohA, OsrbohB, OsrbohD, and OsrbohE showed that mRNA levels of OsrbohA in OsrbohA-RNAi3 were decreased compared to the control cells, whereas no change in OsrbohB, OsrbohD, and OsrbohE expression levels were observed in OsrbohA-RNAi3 (Figure 3A).OsrbohE expression levels were also decreased in OsrbohE-RNAi2; however, mRNA transcripts of the other Osrboh homologs were the same as in control cells(Figure 3B). | *Real-time RT-PCR using specific primer sets for OsrbohA, OsrbohB, OsrbohD, and OsrbohE showed that mRNA levels of OsrbohA in OsrbohA-RNAi3 were decreased compared to the control cells, whereas no change in OsrbohB, OsrbohD, and OsrbohE expression levels were observed in OsrbohA-RNAi3 (Figure 3A).OsrbohE expression levels were also decreased in OsrbohE-RNAi2; however, mRNA transcripts of the other Osrboh homologs were the same as in control cells(Figure 3B). | ||
| − | + | [[File:18-f3.png |center |thumb |10000px |'''''']] | |
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| − | + | ===Immune related-genes regulated under OsrbohA=== | |
| − | + | *EL2, Cht-1, EREBP1, CCD1, and LOX— were selected and the researchers examined the induction patterns of these genes in OsrbohA-RNAi3 and OsrbohE-RNAi2 cultured rice cells. | |
| − | + | <br> | |
==Labs working on this gene== | ==Labs working on this gene== | ||
Revision as of 15:02, 19 August 2016
- Reactive oxygen species(ROS) molecules generated by OsrbohA and OsrbohE regulate different signaling pathways in the plant immune response.
Contents
Annotated Information
Function
- OsrbohA and OsrbohE knockdown lines showed that rapid H2O2 generation is caused by OsrbohA.
- Hypersensitive cell death was decreased only in the OsrbohA knockdown line.
- The researchers further demonstrated that among immune related genes, the induction of EL2 and LOX genes is controlled by ROS generated by OsrbohE, whereas expression of Cht-1 gene is regulated by both OsrbohA and OsrbohE.
Expression
- Real-time RT-PCR using specific primer sets for OsrbohA, OsrbohB, OsrbohD, and OsrbohE showed that mRNA levels of OsrbohA in OsrbohA-RNAi3 were decreased compared to the control cells, whereas no change in OsrbohB, OsrbohD, and OsrbohE expression levels were observed in OsrbohA-RNAi3 (Figure 3A).OsrbohE expression levels were also decreased in OsrbohE-RNAi2; however, mRNA transcripts of the other Osrboh homologs were the same as in control cells(Figure 3B).
- EL2, Cht-1, EREBP1, CCD1, and LOX— were selected and the researchers examined the induction patterns of these genes in OsrbohA-RNAi3 and OsrbohE-RNAi2 cultured rice cells.
Labs working on this gene
- Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST), 8916-5, Takayama Ikoma, Nara 630-0101, Japan;
- Department of Environmental Biology, Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-cho, Nagahama Shiga, 526-0829, Japan.
References
Please input cited references here.
