Difference between revisions of "Os01g0907400"
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| − | + | The rice '''''Os01g0907400''''' was reported as '''''JMJ705''''' in 2013 <ref name="ref1" /> by researchers from China and France. | |
==Annotated Information== | ==Annotated Information== | ||
| + | [[File:Os01g0907400.png|right|thumb|327px|'''Figure 2.''' ''JMJ705 Overexpression Produces a Leaf Lesion–Mimic Phenotype at the Mature Stage..<ref name="ref1" />.'']] | ||
| + | ===Gene Symbol=== | ||
| + | *'''''Os01g0907400''''' '''<=>''' '''''JMJ705,OsJMJ705''''' | ||
| + | |||
===Function=== | ===Function=== | ||
| − | + | * '''''JMJ705''''' encodes a histone lysine demethylase that specifically reverses H3K27me2/3. | |
| + | * Overexpression of '''''JMJ705''''' reduces the resting level of H3K27me2/3 resulting in preferential activation of H3K27me3-marked biotic stress responsive genes and enhances rice resistance to the bacterial blight disease pathogen Xanthomonas oryzae pathovar oryzae. | ||
| + | * Mutation of '''''JMJ705''''' reduces plant resistance to the pathogen. | ||
| + | * '''''JMJ705''''' is involved in methyl jasmonate–induced dynamic removal of H3K27me3 and gene activation. | ||
| + | * The results suggest that '''''JMJ705''''' is a biotic stress-responsive H3K27me2/3 demethylase that may remove H3K27me3 from marked defense-related genes and increase their basal and induced expression during pathogen infection. | ||
| + | |||
| + | ===Phenotypic analysis=== | ||
| + | * To study whether increased expression of '''''JMJ705''''' could reduce the resting level of H3K27me3 and affect gene expression and plant growth, we produced transgenic rice plants overexpressing JMJ705-FLAG under the strong maize ubiquitin promoter in the Zhonghua11 (ZH11) background (Figure 2A). | ||
| + | * Protein gel blot analysis indicated that the overexpression of the gene resulted in a reduction of the overall H3K27me2/3 levels in the transgenic plants compared with wild type (Figure 1D). The overexpression transgenic lines displayed a leaf lesion-mimic phenotype at the mature stage (Figures 2A and 2B). | ||
===Expression=== | ===Expression=== | ||
| − | + | * RT-PCR analysis indicated that '''''JMJ705''''' was expressed in all tested tissues/organs, with a relatively higher level in rice leaves, and was found to be induced by NaCl, abscisic acid, ethylene (ACC), and JA (see Supplemental Figure 2 online), suggesting that JMJ705 might be involved in stress responses. | |
| − | + | * The expression of '''''JMJ705''''' is induced by stress signals and during pathogen infection. | |
| − | |||
| − | |||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China | |
| + | * Institut de Biologie des Plantes Unité Mixte de Recherche 8618 Université Paris-sud 11, 91405 Orsay, France | ||
==References== | ==References== | ||
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Jumonji C Domain Protein JMJ705-Mediated Removal of | ||
| + | Histone H3 Lysine 27 Trimethylation Is Involved in | ||
| + | Defense-Related Gene Activation in Rice | ||
| + | </ref> | ||
| + | |||
| + | </references> | ||
==Structured Information== | ==Structured Information== | ||
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]] | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]] | ||
Latest revision as of 14:38, 9 October 2016
The rice Os01g0907400 was reported as JMJ705 in 2013 [1] by researchers from China and France.
Contents
Annotated Information
Figure 2. JMJ705 Overexpression Produces a Leaf Lesion–Mimic Phenotype at the Mature Stage..[1].
Gene Symbol
- Os01g0907400 <=> JMJ705,OsJMJ705
Function
- JMJ705 encodes a histone lysine demethylase that specifically reverses H3K27me2/3.
- Overexpression of JMJ705 reduces the resting level of H3K27me2/3 resulting in preferential activation of H3K27me3-marked biotic stress responsive genes and enhances rice resistance to the bacterial blight disease pathogen Xanthomonas oryzae pathovar oryzae.
- Mutation of JMJ705 reduces plant resistance to the pathogen.
- JMJ705 is involved in methyl jasmonate–induced dynamic removal of H3K27me3 and gene activation.
- The results suggest that JMJ705 is a biotic stress-responsive H3K27me2/3 demethylase that may remove H3K27me3 from marked defense-related genes and increase their basal and induced expression during pathogen infection.
Phenotypic analysis
- To study whether increased expression of JMJ705 could reduce the resting level of H3K27me3 and affect gene expression and plant growth, we produced transgenic rice plants overexpressing JMJ705-FLAG under the strong maize ubiquitin promoter in the Zhonghua11 (ZH11) background (Figure 2A).
- Protein gel blot analysis indicated that the overexpression of the gene resulted in a reduction of the overall H3K27me2/3 levels in the transgenic plants compared with wild type (Figure 1D). The overexpression transgenic lines displayed a leaf lesion-mimic phenotype at the mature stage (Figures 2A and 2B).
Expression
- RT-PCR analysis indicated that JMJ705 was expressed in all tested tissues/organs, with a relatively higher level in rice leaves, and was found to be induced by NaCl, abscisic acid, ethylene (ACC), and JA (see Supplemental Figure 2 online), suggesting that JMJ705 might be involved in stress responses.
- The expression of JMJ705 is induced by stress signals and during pathogen infection.
You can also add sub-section(s) at will.
Labs working on this gene
- National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China
- Institut de Biologie des Plantes Unité Mixte de Recherche 8618 Université Paris-sud 11, 91405 Orsay, France
