Difference between revisions of "Os02g0527600"

Revision as of 05:08, 10 October 2016

The rice Os02g0527600 was reported as OsCTR2 in 2013 ^[1] by researchers from China.

The osctr2 loss-of-function mutation and expression of the 35S:OsCTR2 1–513 transgene that encodes the OsCTR2 N terminus (residues 1–513) revealed several and many aspects, respectively, of ethylene-induced growth alteration in rice.
The rice OsCTR2 is closely related to CTR1 and negatively regulates ethylene signalling.

To evaluate whether OsCTR1 and OsCTR2 have any roles in ethylene-receptor signalling, the researchers ectopically expressed each gene in a ctr1-1 loss-of-function mutant for complementation testing.
The expression of OsCTR2 partially rescued the ctr1-1 ethylene response phenotype. Light-grown ctr1-1 seedlings produced small, compact cotyledons and a short root; consistently, ctr1-1 transgenic lines expressing CTR1p:OsCTR2 produced larger cotyledons and a longer root than ctr1-1 seedlings (Fig. 2E). At the adult stage, expression of the CTR1p:OsCTR2 transgene largely rescued the ctr1-1 growth inhibition phenotype, and the transgenic lines produced a normal rosette, as in wild-type plants (Col-0)

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 * The '''''osctr2''''' loss-of-function mutation and expression of the 35S:OsCTR2 1–513 transgene that encodes the '''''OsCTR2''''' N terminus (residues 1–513) revealed several and many aspects, respectively, of ethylene-induced growth alteration in rice.
 * The rice '''''OsCTR2''''' is closely related to CTR1 and negatively regulates ethylene signalling.
+===Phenotypic analysis===
+* To evaluate whether '''''OsCTR1''''' and '''''OsCTR2''''' have any roles in ethylene-receptor signalling, the researchers ectopically expressed each gene in a ctr1-1 loss-of-function mutant for complementation testing.
+* The expression of '''''OsCTR2''''' partially rescued the ctr1-1 ethylene response phenotype. Light-grown ctr1-1 seedlings produced small, compact cotyledons and a short root; consistently, ctr1-1 transgenic lines expressing CTR1p:OsCTR2 produced larger cotyledons and a longer root than ctr1-1 seedlings (Fig.  2E). At the adult stage, expression of the CTR1p:OsCTR2 transgene largely rescued the ctr1-1 growth inhibition phenotype, and the transgenic lines produced a normal rosette, as in wild-type plants (Col-0)
 ===Expression===