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| − | {{JaponicaGene|
| + | The rice '''''Os02g0527600''''' was reported as ''''' OsCTR2 ''''' in 2013 <ref name="ref1" /> by researchers from China. |
| − | GeneName = Os02g0527600|
| |
| − | Description = Similar to MAP kinase kinase kinase (Fragment)|
| |
| − | Version = NM_001053539.2 GI:297599335 GeneID:4329527|
| |
| − | Length = 7476 bp|
| |
| − | Definition = Oryza sativa Japonica Group Os02g0527600, complete gene.|
| |
| − | Source = Oryza sativa Japonica Group
| |
| | | | |
| − | ORGANISM Oryza sativa Japonica Group
| + | ==Annotated Information== |
| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
| + | [[File:41-Os02g0527600.png|right|thumb|427px|'''Figure 5.''' ''Subcellular localization of GFP–OsCTR2. Fluorescence of GFP–OsCTR2 (green) and ER-rk (red) in tobacco (A, B) and onion epidermal (C, D) cells. The cell for the co-localization study is indicated with an arrowhead in (A) and (B).<ref name="ref1" />.'']] |
| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
| + | ===Gene Symbol=== |
| − | clade; Ehrhartoideae; Oryzeae; Oryza.
| + | *'''''Os02g0527600''''' '''''<=>''''' '''''OsCTR2''''','''''CTR2''''' |
| − | |
| + | ===Function=== |
| − | Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
| + | * The '''''osctr2''''' loss-of-function mutation and expression of the 35S:OsCTR2 1–513 transgene that encodes the '''''OsCTR2''''' N terminus (residues 1–513) revealed several and many aspects, respectively, of ethylene-induced growth alteration in rice. |
| − | AP = Chromosome 2:20182343..20189818|
| + | * The rice '''''OsCTR2''''' is closely related to CTR1 and negatively regulates ethylene signalling. |
| − | CDS = 20182343..20182418,20183818..20183921,20184036..20184098,20184186..20184317,20184405..20184473<br>,20184921..20185019,20185170..20185254,20185358..20185458,20185762..20185830<br>,20186103..20186143,20186811..20187164,20187734..20187942,20188279..20188332<br>,20188931..20189229,20189312..20189818|
| + | |
| − | GCID = <gbrowseImage1>
| + | ===Phenotypic analysis=== |
| − | name=NC_008395:20182343..20189818
| + | * To evaluate whether '''''OsCTR1''''' and '''''OsCTR2''''' have any roles in ethylene-receptor signalling, the researchers ectopically expressed each gene in a ctr1-1 loss-of-function mutant for complementation testing. |
| − | source=RiceChromosome02
| + | * The expression of '''''OsCTR2''''' partially rescued the ctr1-1 ethylene response phenotype. Light-grown ctr1-1 seedlings produced small, compact cotyledons and a short root; consistently, ctr1-1 transgenic lines expressing CTR1p:OsCTR2 produced larger cotyledons and a longer root than ctr1-1 seedlings (Fig. 2E). |
| − | preset=GeneLocation
| + | * At the adult stage, expression of the CTR1p:OsCTR2 transgene largely rescued the ctr1-1 growth inhibition phenotype, and the transgenic lines produced a normal rosette, as in wild-type plants (Col-0) |
| − | </gbrowseImage1>|
| + | |
| − | GSID = <gbrowseImage2>
| + | ===Subcellular Localization === |
| − | name=NC_008395:20182343..20189818
| + | * Researchers have already studied the subcellular localization of '''''OsCTR2''''' with GFP-fused OsCTR2. |
| − | source=RiceChromosome02
| + | * Green (GFP–OsCTR2) and red (ER-rk) fluorescence was observed mainly surrounding the nucleus, at the cell edge of tobacco cells, with an uneven, discrete pattern (Fig. 5A), and the ER network was also observed (Fig. 5B). |
| − | preset=GeneLocation
| + | * To obtain other evidence supporting localization of '''''OsCTR2''''' at the ER, GFP–OsCTR2 was transiently co-expressed with ER-rk in onion epidermal cells by particle bombardment. Consistently, the fluorescence of GFP–OsCTR2 and ER-rk co-localized (Fig. 5C). The nucleus was surrounded by GFP–OsCTR2 and ER-rk (Fig. 5D), which is consistent with the close association of the ER with the outer membrane of the nuclear envelope. |
| − | </gbrowseImage2>| | + | |
| − | CDNA = <cdnaseq>atggtgctcggcggcggcggcggcggcgggaggcgtgcctcgccagggtctgcgcctccgcccgccgcgcccgctgccgtggcgtacagcctcctcgccacctccccgcccgccagcatcggcaacggtggcggctcgccgcactgcgacgacggcgacgcgtcccgcgggctcggcgtggcggactggctgcggctgcagcggcactcgagcgggagcagcgccggcgacgacggggatggcttctcctccgtctccaccctcgccactgccgacaagggcggcgaccccgccgatcgcccggccggcagcagcggcggcggcggcagcaagagctgggcgcagcaggcggaggaggcgtaccagctccagctcgccctcgcgctccgtctctgctccgaggcctccaccgcccccgaccccaacttcctcgactccgccgtggcggcggccgacgaccaccaccgcgacgcgccctcgccccaatccctctcgcatcgcttctgggtgaatggcagcttgtcctactccgacaaggtcctggatggcttctacctcattcatgggatggatcccttcgtatggacgctctgcaatgacttgcgcgatggcgcccgagtgccctcgatcgagtcgctcaaggctatgaatcccaccgagtcatccgtggaggtggtcctcatagatagggtggtcgactatgatctgaggcaactgataagcacggcgattgacgtttctcggagccgcgctgactcaagggaaatcaccactcgccttgctggcattgtgtcctcaaagatggggtactgcaaatctgctggtgctgcttcatgcctggtgcactttggtaatgacagggaatatctgattgatttgatagggaacccaggcttcttatctgaacctgactccctcttaaatgggctctcatccatttcagtttcttcaccattgcgcccaccaaaatacaattcagctgatattgttaataacttcaagtcattggcgaagcaatatttccttgattgccaatcgctaaatatgatgttcaatgatccagcagcagggacggttgttgacttagatgaggccatgggatcaaatattggtccaaacttatcgcctgcaacaaacagtgattttcaagctaacttttcacatcgtagtcgtggtgcccagtccagtggtcaagatggcaattttcttattcagaagaggtgcatcagcaggatcctccctaaaaattgttatagttatttccactttccatcatccaaagttgataatactgatgaatatttcagttctcctgaggatacacaatctgcacaaagtgaccccttcagtgatatatctctcgacattgaagacttgattattccttggagtgaacttgttttgaaagagaaaattggagctggctcatttggaaccgtgcaccgtgcggattggaatggctcggatgttgctgtaaagattttgatggagcaagacttccacccagagcgtctcaaagaatttcttagggaggttgcaattatgaaaagtctgcgccatccaaacattgtcctttttatgggtgctgttactcagccaccaaaactttcgattgtgacagaatatctatcaagaggtagcctatacaggattttgcacaagcatggtgcaagggaaaatctagatgagaagcggcgattgagtatggcttttgacgtggctaaggggatgaattaccttcacaagcgcaatcctcctatagtccaccgggatctaaagtcaccaaatctgttggttgataaaaagtacactgtgaaggtctgtgactttggtctctccagattgaaggcaaacacatttttgtcttccaagactgcagcagggactcctgagtggatggcaccagaagttatacgagatgagccatcaaatgagaagtctgatgtctacagctttggtgttatcctatgggagctcatgacattgcaacaaccatggagtactttgaatccagctcaggttgtagcagctgttggttttaatgggcgaaggcttgaaattccaagtagcgttgatccaaaaagaaccctggcgacgaccgtcttttgccagtatcatggaatccctgaaacctctaatcagaactccccatcaacttcaagaggatatcagttaacggttcatggatcgtgctggatgtggtgcatgcagtggaggtctcgactggcggcggcgaccagatcggcgttcgcctgtggttga</cdnaseq>|
| + | |
| − | AA = <aaseq>MVLGGGGGGGRRASPGSAPPPAAPAAVAYSLLATSPPASIGNGG GSPHCDDGDASRGLGVADWLRLQRHSSGSSAGDDGDGFSSVSTLATADKGGDPADRPA GSSGGGGSKSWAQQAEEAYQLQLALALRLCSEASTAPDPNFLDSAVAAADDHHRDAPS PQSLSHRFWVNGSLSYSDKVLDGFYLIHGMDPFVWTLCNDLRDGARVPSIESLKAMNP TESSVEVVLIDRVVDYDLRQLISTAIDVSRSRADSREITTRLAGIVSSKMGYCKSAGA ASCLVHFGNDREYLIDLIGNPGFLSEPDSLLNGLSSISVSSPLRPPKYNSADIVNNFK SLAKQYFLDCQSLNMMFNDPAAGTVVDLDEAMGSNIGPNLSPATNSDFQANFSHRSRG AQSSGQDGNFLIQKRCISRILPKNCYSYFHFPSSKVDNTDEYFSSPEDTQSAQSDPFS DISLDIEDLIIPWSELVLKEKIGAGSFGTVHRADWNGSDVAVKILMEQDFHPERLKEF LREVAIMKSLRHPNIVLFMGAVTQPPKLSIVTEYLSRGSLYRILHKHGARENLDEKRR LSMAFDVAKGMNYLHKRNPPIVHRDLKSPNLLVDKKYTVKVCDFGLSRLKANTFLSSK TAAGTPEWMAPEVIRDEPSNEKSDVYSFGVILWELMTLQQPWSTLNPAQVVAAVGFNG RRLEIPSSVDPKRTLATTVFCQYHGIPETSNQNSPSTSRGYQLTVHGSCWMWCMQWRS RLAAATRSAFACG</aaseq>|
| + | |
| − | DNA = <dnaseqindica>7401..7476#5898..6001#5721..5783#5502..5633#5346..5414#4800..4898#4565..4649#4361..4461#3989..4057#3676..3716#2655..3008#1877..2085#1487..1540#590..888#1..507#atggtgctcggcggcggcggcggcggcgggaggcgtgcctcgccagggtctgcgcctccgcccgccgcgcccgctgccgtggcgtacagcctcctcgccacctccccgcccgccagcatcggcaacggtggcggctcgccgcactgcgacgacggcgacgcgtcccgcgggctcggcgtggcggactggctgcggctgcagcggcactcgagcgggagcagcgccggcgacgacggggatggcttctcctccgtctccaccctcgccactgccgacaagggcggcgaccccgccgatcgcccggccggcagcagcggcggcggcggcagcaagagctgggcgcagcaggcggaggaggcgtaccagctccagctcgccctcgcgctccgtctctgctccgaggcctccaccgcccccgaccccaacttcctcgactccgccgtggcggcggccgacgaccaccaccgcgacgcgccctcgccccaatccctctcgcatcgcttctgggttcgcactcaccgcgcgcacacaccagttcaccgaaagatgtataccgattctccaagtgaccatctcgctgtcttggcaggtgaatggcagcttgtcctactccgacaaggtcctggatggcttctacctcattcatgggatggatcccttcgtatggacgctctgcaatgacttgcgcgatggcgcccgagtgccctcgatcgagtcgctcaaggctatgaatcccaccgagtcatccgtggaggtggtcctcatagatagggtggtcgactatgatctgaggcaactgataagcacggcgattgacgtttctcggagccgcgctgactcaagggaaatcaccactcgccttgctggcattgtgtcctcaaagatggggtgagagactgggagttgataaggaaattgccacttttggtacattttctctgtcttacatgtgtctcactaatgttgaaggggttccgtagcatctaccgaagaacatgaactgtgtccccggtggagagactctgccgggttcctgaagattagttcaggttctgttgtgcttccaattgggaagttatcaatcggtctttgccggcaccgatcgttgctgtttaaggtcaccttgcttctatcgctatgttctaaaatctattaaatcttcgtatttagtggcaatattttccatgttagcgctggaactttgtttttatgattttacaaagctcatcatgcaatattcctgattgccctaaaatacattagatggtgaattatagtgatattgatgcactcacaagcatacataggagctgcactactttaaaagatcagtttcttatgatcctatttcattttagttgccagtcctgatactctcttgtctcttcaatttgtttttgataataatcctattctgttttctttggtggggaaagaccttagcagacactatcagtctcccatgtcgagttgtgaggggatgcaggtactgcaaatctgctggtgctgcttcatgcctggtgcactttggtaatgacaggtttgtaatcctgatgaatgacttgcactatttctacagattttgggtcatcaaaggttaaaaaagagagagaatatgaagaaagaattgtgccccagttacaccaattacattccttgtctgatctaacaatgtcatagaaagagggtataacattgttataaatgtgaaaatggaaaaaatataaatggaacttatgttctcaagttatctgtacaatcttgctccgaatattctttatttttccagttttctatgttcttttacactctccagttttcaatgttgaaatagcatctaatcacttcttgacttctattttatttttggtatcagggaatatctgattgatttgatagggaacccaggcttcttatctgaacctgactccctcttaaatgggctctcatccatttcagtttcttcaccattgcgcccaccaaaatacaattcagctgatattgttaataacttcaagtcattggcgaagcaatatttccttgattgccaatcgctaaatatgatgttcaatgatccagcagcaggtcagatcatacttttctgtcttctaaatactatttatttaagtactcttcacatgatgtgatattaatagtaatatctcaaatgggctgatctgtataggaagagattttgacattattaaacttttaaaattaacagctgatgctgctgtgagcatgttgtgttctatgttcaaatagatagtttttagatttttttttgctgggagaaactttatgtcattgacatctagttttgatataggcaacaatctttcagcacaatcattttagatgttaaaacttaaaacttttttggatgaaatagtatggtttacaatagttcttagctgtagtttcacaattcaggtctagtcattttctcctgaatggtcttcaaaagttttaaaagaaaagatggaaaatgaactgcctactattctaagcaagcaaactgagtaactgaccaattgatattttttatcatcactatttcaattcttgaattggaatttcagaaagcaggactaaaaaaaatatttaagatcttttcaaaagattgtgtcttttactattttgaatagtttcagggacggttgttgacttagatgaggccatgggatcaaatattggtccaaacttatcgcctgcaacaaacagtgattttcaagctaacttttcacatcgtagtcgtggtgcccagtccagtggtcaagatggcaattttcttattcagaagaggtgcatcagcaggatcctccctaaaaattgttatagttatttccactttccatcatccaaagttgataatactgatgaatatttcagttctcctgaggatacacaatctgcacaaagtgaccccttcagtgatatatctctcgacattgaagacttgattattccttggagtgaacttgttttgaaagagaaaattggagctggtacgtttttgttttttaatagtgtttttgtacgatgtacttagttttttttatctctattatactacacatgacctccaatcaacaaaacgaattgttctggcttagcttttttttttaaaaaaaaaaaaaatctctataccacatacaacctccaatcaataaagcaagttgttctggcttacatgagcaagtacaatgaagagtgacattgggcattcttttgtatcatttagcctcttctaaccctactaattacataagcatgttattaatgaaagttaaccacaacgtttagtgtgagactacaaagaatatttgcaattaactccatggattatagtaccaatgcatgatattaaatagggccaatcttgtacaaaaccacaaattactgaaagttcggtatgcgcatccaaggcacaaggctagaactaggtgccttcttcactagagttttaccacagttatgcacaaggctagaacaaggcgcaaggcaaattaatagcatgcagtttttattttacacatattttgcgaaggtgaacaagttgaatattttggacatccgtgacctcacattgtgaaaattaatgaatgatcatgaagctgcctatttccagattttgatatttttgtagtgtgcactaattgagaaaaatgacaggctcatttggaaccgtgcaccgtgcggattggaatggctcggtatgtctctttttattacaagcacattttacagtgtgcttatgtgttacaagttactacttgaatagtgaccggtctaacatgtactgcatacaactacgcctcctgtatttcctttttggtgtgccaatatatgattttaggagagatgttaacaaatgttgtttcaataacctgaaacactcaaccgtcccacagttatgtacttattcttcatgtgaggaattgaggataggagagctcaattaacactcttgcctttcatgtctcaggatgttgctgtaaagattttgatggagcaagacttccacccagagcgtctcaaagaatttcttagggaggtataacatcagaatttccagtggacaatttatattggtcttcctttgcgactaccaaatctgatagggatgaaagcagcagcgctttgaattttcctacatataatggacatgttatacaacgatgtgcatgactagatgcttctgaactgatctttcctctgcaacaaagttaatgcccagtattctttctttttttaacagagaatgttaagatgttgccccattcaaggtcacatgtataagttgatttaatcatgtaatgtccatgtcacttaacatgttaggtattctcctctgtaggttgcaattatgaaaagtctgcgccatccaaacattgtcctttttatgggtgctgttactcagccaccaaaactttcgattgtgacagaatatctatcaaggtgtgttgaactttctgattactatttcaaaattgatgtaaactatgtgcttacctctagtctagcttgtagttcccacataagttttttttcttgaatgcagaggtagcctatacaggattttgcacaagcatggtgcaagggaaaatctagatgagaagcggcgattgagtatggcttttgacgtggtaaaatcatatatacttatctcaatacagaatttaggtctaacagaatgactggacatatgctttatataaagtaaaggttgaccgtagaatcaaaattgtaggtgcttctttataaaaatcattgattttgtgtaccttcgtatccaggctaaggggatgaattaccttcacaagcgcaatcctcctatagtccaccgggatctaaagtcaccaaatctgttggttgataaaaagtacactgtgaaggtatatccttttacccattttgccatattttaagacctgtttgatttcacattactctttgtggtactttttgttattttttctactttgtttttggacaaagtataacttccggcctctgccccatgactccgccaccacaccaatggtttttccccttttaaccaaagatgcacatggccatatttgtggacaccagaatttgaactctagtgggtggaatcatgcacccatgactccgccaccacaccaatggcgtttccccttttatgagaaaactttatcttcaattactctatgtggtactttattattattattttttctacttgaatgggtatagttgttatgatcattaggattatctttctttctaatataattggcagttgttccctatttaatcttatctccaactaacaacaaatttcccctacaatgctgcaggtctgtgactttggtctctccagattgaaggcaaacacatttttgtcttccaagactgcagcagggactgtaagttcatattaccgttacttttatttgttgattttttttcagctaaatcgtgctagtaagtgctttcgatgtaatattctctagcctgagtggatggcaccagaagttatacgagatgagccatcaaatgagaagtctgatgtctacagctttggtgttatcctatgggagctcatgacattgcaacaaccatggagtactttgaatccagctcaggtttgtatctcctgttttcttcatcacacggatattttccaacttcggacgaactgaattgaacaaagtctacctgttcacttgcaggttgtagcagctgttggttttaatgggcgaaggcttgaaattccaagtagcgttgatccaaaagtggcggcaataatggaatcctgctggacaaagtatctataatatactgttatatgtttcttctcacaacttgtagccaagttggtttgatgaatattttatttttatttgcagagaaccctggcgacgaccgtcttttgccagtatcatggaatccctgaaacctctaatcagaactccccatcaacttcaagaggatatcagttaacggttcatgggtaccttcaaaaccctgaagatacgagcccttgacaacttacaaaggtgactagctatcttagtcgttaacttcttaggtttgaattcagacatttttctttcttttggcttccgattattgtcagtaatatttttgctccgaaagctatttaagcttatagttatatctcttccctggacggctaaattagttatttcactggtaaagttcattttcatgataatgtatatattcataccatgctataccgtacatacaaaatgaacagactccttaaataaagcccatcatttttttttttgcctcagttaatgcttttaggtgtattgttcatgcatgctgttgctagtatgattgcttgtgaaattccattgcattgtctttggtccctagcactgtctaaattgtccttgagaagaaaaggactgtgtgtctgtgtatgtaaagatgtttatggaaatgttcgatacaatatccccaaaatcaaaaccttttcacaacatattttatctttggatttaatccatctatcttttgttcaaaaatccttttagcctcttttttgttttaattatacggtagacacacgcgcgcacacaccactacatacacaatgcaacaccaatgcaacacttacattccgaatgcatcctcgtacatatgctctaagagactgaaaccaacggtacatctccattgaaagctcacttgcatgtttgtaatatttgtgggcactaggatttaaacccatcgtaccactagcctctttaaaacatggaattctaaaaaaaataatacattagtaaaacactggattagtgtgtcatttattgttcaatcctcagtaattctataacatttggaattattacatcatgctgcttggatgctcgccattagaaaaatacagtacttttaggagaaaggcgcgatggagaacttctacgtacctggtcatatctcatgttggagatcctcaaaagctgttaccacttaccatcacgcgcacaatcacaaagcattcccttaccataactcacactaaaagtttttcaaaaacagccagtctattttcaaattactctcccttggtccaaaaaaaatgaatttttttagtgtacctttaaataaatatggaccgtagaagatatgagatctaagggtggaaattcgttgttactacacctagtataagggtgtagtagtgtagtacaagggtgactgtggaaaaattatagatggggtgtattatgtaattgcgcgtgcactgcaatagggccggctcaattgattctacgtcccgatttgggattagttgattaattaacatatacccgaggaaaaaaagggctgcagatcctcgtggctgtggcgttggtcgtcgtggatggttgcagatcgtgctggatgtggtgcatgcagtggaggtctcgactggcggcggcgaccagatcggcgttcgcctgtggttga</dnaseqindica>|
| + | You can also add sub-section(s) at will. |
| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001053539.2 RefSeq:Os02g0527600]|
| + | |
| − | }}
| + | ==Labs working on this gene== |
| − | [[Category:Genes]] | + | * National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, PR China |
| − | [[Category:Japonica mRNA]]
| + | |
| − | [[Category:Oryza Sativa Japonica Group]] | + | ==References== |
| − | [[Category:Japonica Genes]] | + | <references> |
| − | [[Category:Japonica Chromosome 2]]
| + | * <ref name="ref1"> |
| − | [[Category:Chromosome 2]]
| + | Wang Q, Zhang W, Yin Z, Wen CK. Rice CONSTITUTIVE TRIPLE-RESPONSE2 is involved |
| | + | in the ethylene-receptor signalling and regulation of various aspects of rice |
| | + | growth and development. J Exp Bot. 2013 Nov;64(16):4863-75. doi: |
| | + | 10.1093/jxb/ert272. Epub 2013 Sep 4. PubMed PMID: 24006427; |
| | + | </ref> |
| | + | </references> |
| | + | |
| | + | ==Structured Information== |
| | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]] |
