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| − | Please input one-sentence summary here.
| + | The rice '''''Os03g0686900''''' was reported as '''''FLO6''''' in 2014 <ref name="ref1" /> by researchers from China. |
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| | ==Annotated Information== | | ==Annotated Information== |
| | + | [[File:49-Os03g0686900.png|right|thumb|427px|'''Figure 1.''' ''Phenotype of the flo6 mutant.<ref name="ref1" />.'']] |
| | + | ===Gene Symbol=== |
| | + | *'''''Os03g0686900''''' '''''<=>''''' '''''FLO6''''','''''OsFLO6''''' |
| | ===Function=== | | ===Function=== |
| − | Please input function information here.
| + | * '''''FLO6''''' encodes a ubiquitously expressed CBM48-containing protein. |
| | + | * '''''FLO6''''' can interact with isoamylase1 (ISA1) both in vitro and in vivo, whereas ISA1 does not bind to starch directly. |
| | + | * '''''FLO6''''' may act as a starch-binding protein involved in starch synthesis and compound granule formation through a direct interaction with ISA1 in developing rice seeds. |
| | + | ===Phenotypic analysis=== |
| | + | * The '''''flo6''''' mutant, recovered from tissue culture of the japonica rice cv. Nipponbare, had no visible differences with wild-type plants during the seedling, tillering and heading stages, and mature plants were slightly shorter than wild-type plants; however, the flo6 mutant displayed a remarkably slower grain-filling rate (Figure 1a). |
| | + | * This difference became apparent from approxi- mately 15 days after flowering (DAF), and was maintained until seed maturation (Figure 1a). Consistent with the lower grain-filling rate, starch synthesis in the flo6 endo- sperm was also decreased (Figure 1b). |
| | + | * At the mature stage, flo6 seeds had smaller and floury endosperms (Figure 1c,e), compared with that of the wild type (Figure 1d,f). |
| | + | * The interior of the endosperm in the mature grains was floury white (Figure 1f), and the SGs of the mutant grains were loosely packed (Figure 1g–j). |
| | + | * Thus, the mutation in '''''FLO6''''' affects starch accumulation during endo- sperm development. |
| | + | ===Subcellular localization=== |
| | + | * The '''''FLO6''''' protein was localized to the plastid by the N–terminal transit peptide. |
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| − | ===Expression===
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| − | Please input expression information here.
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| − |
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| − | ===Evolution===
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| − | Please input evolution information here.
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| | You can also add sub-section(s) at will. | | You can also add sub-section(s) at will. |
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| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | Please input related labs here.
| + | * State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China, |
| | + | * College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China |
| | + | * National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
| | | | |
| | ==References== | | ==References== |
| − | Please input cited references here.
| + | <references> |
| − | | + | * <ref name="ref1"> |
| | + | Peng C, Wang Y, Liu F, Ren Y, Zhou K, Lv J, Zheng M, Zhao S, Zhang L, Wang C, |
| | + | Jiang L, Zhang X, Guo X, Bao Y, Wan J. FLOURY ENDOSPERM6 encodes a CBM48 |
| | + | domain-containing protein involved in compound granule formation and starch |
| | + | synthesis in rice endosperm. Plant J. 2014 Mar;77(6):917-30. doi: |
| | + | 10.1111/tpj.12444. Epub 2014 Mar 8. PubMed PMID: 24456533. |
| | + | </ref> |
| | + | </references> |
| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]] |
| − | GeneName = Os03g0686900|
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| − | Description = Glycoside hydrolase, family 13, N-terminal domain containing protein|
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| − | Version = NM_001057471.2 GI:297601494 GeneID:4333759|
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| − | Length = 5099 bp|
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| − | Definition = Oryza sativa Japonica Group Os03g0686900, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| − | | |
| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
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| − | AP = Chromosome 3:28168759..28173857|
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| − | CDS = 28168759..28169279,28169371..28169424,28169518..28169639,28170895..28171092,28171169..28171404<br>,28171816..28171896,28171940..28172085,28172165..28172312,28173010..28173078<br>,28173155..28173205,28173323..28173364,28173846..28173857|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008396:28168759..28173857
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| − | source=RiceChromosome03
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008396:28168759..28173857
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| − | source=RiceChromosome03
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>atgctccccctcctcctccccctccccgtgacccctcctcctcctctcccctccccaaccctaaccctagcccccgcctccgctcctcgccgacgcctcgtcctcctcgccgccgcggcgccgcaccaccaccatcaccaccgccgccgccgcgtctaccgtcgccagcgcgcggcgccgacgcagacgcgggcgccgaggaggacgttgtcggcgtccaatgcggcccgcggggaggaggacctcgaggaggcgatctacgagttcatgcggcggtccgacaagccgggggcgttccccacccgcgccgagctcgtcgccgcggggagggccgacctcgccgccgccgtggacgcctgcggcggctggctctcgctcgggtggtcctccggtggcgcggaggcaggcagggcctcctcgtcggtcggcgtgcaccctgactaccctcccgaggcgggggcggcggcggcggcggggggcgcctccgacctagcgcagggagccgtgtgggcgtcgtccagggaggcggaagcttcgccgtccgggcgccagccggagacggaggaggaggagacggagacgaaatttgggactgggttggacgggatgcttaccaggctgcagagggaaagggagagggtccggccgccgctcccacggagcagcgatggagcgggaggggaacgcgacaatgttgctttaatgggtcagagtggagcacccagtcactctgcaactggtggcaggtacactccaaaggtacctgataatggaaatatacatagttatcatcctcaaaatggagcactagaacataacaaaagctcaaagagtttgactaatgatgcatggcgaacatggtcccttgacaagggtggtttttcagattttcaagctgctgaaatccattcaactaacagcaggaaatcatttagacatgatggcctagacattcttgcacaagatgacgtgcacgggccatctaatggtgtggctgtacatgattatgatattaatgatgtagactctgaaagagacgatatacatgcacgccttcaaaatttggagttggatcttactgccgctcttcatacattaagatcaagattcgacaaggttatatcagatatgttacagggcatacaaatacatgaaacactagtggatgaagttgtgctttgtgcctctcaccttgatcttgccagaggcacttccgaaggtgacggagcaaaagcaccaaatgggctctccgacgattgggaatttgaagagaccaaagtaatgcaagctcaggaagagctgcggtcaatccgtgcaaaaatagcagtattagaaggaaagatggcactggagataattgagaagaacaaaataattgaagagaaacaaagaaggcttgatgaagctgagaaggccttgagtgagctccgtactgtttatattgtgtggtctaatcctgcttcagaggttctattgactggatcttttgatggctggacaagccagagaagaatggaaagatcagaaagaggcaccttttccttaaacctgaggttgtatcctggtagatatgagattaaatttattgttgatggtgtttggaggaatgatcctctgcgcccccttgccaatccatccagaatggaaacaaaaaaaatgagtgagctggttgaactctga</cdnaseq>|
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| − | AA = <aaseq>MLPLLLPLPVTPPPPLPSPTLTLAPASAPRRRLVLLAAAAPHHH HHHRRRRVYRRQRAAPTQTRAPRRTLSASNAARGEEDLEEAIYEFMRRSDKPGAFPTR AELVAAGRADLAAAVDACGGWLSLGWSSGGAEAGRASSSVGVHPDYPPEAGAAAAAGG ASDLAQGAVWASSREAEASPSGRQPETEEEETETKFGTGLDGMLTRLQRERERVRPPL PRSSDGAGGERDNVALMGQSGAPSHSATGGRYTPKVPDNGNIHSYHPQNGALEHNKSS KSLTNDAWRTWSLDKGGFSDFQAAEIHSTNSRKSFRHDGLDILAQDDVHGPSNGVAVH DYDINDVDSERDDIHARLQNLELDLTAALHTLRSRFDKVISDMLQGIQIHETLVDEVV LCASHLDLARGTSEGDGAKAPNGLSDDWEFEETKVMQAQEELRSIRAKIAVLEGKMAL EIIEKNKIIEEKQRRLDEAEKALSELRTVYIVWSNPASEVLLTGSFDGWTSQRRMERS ERGTFSLNLRLYPGRYEIKFIVDGVWRNDPLRPLANPSRMETKKMSELVEL</aaseq>|
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| − | DNA = <dnaseqindica>1..521#613..666#760..881#2137..2334#2411..2646#3058..3138#3182..3327#3407..3554#4252..4320#4397..4447#4565..4606#5088..5099#atgctccccctcctcctccccctccccgtgacccctcctcctcctctcccctccccaaccctaaccctagcccccgcctccgctcctcgccgacgcctcgtcctcctcgccgccgcggcgccgcaccaccaccatcaccaccgccgccgccgcgtctaccgtcgccagcgcgcggcgccgacgcagacgcgggcgccgaggaggacgttgtcggcgtccaatgcggcccgcggggaggaggacctcgaggaggcgatctacgagttcatgcggcggtccgacaagccgggggcgttccccacccgcgccgagctcgtcgccgcggggagggccgacctcgccgccgccgtggacgcctgcggcggctggctctcgctcgggtggtcctccggtggcgcggaggcaggcagggcctcctcgtcggtcggcgtgcaccctgactaccctcccgaggcgggggcggcggcggcggcggggggcgcctccgacctagcgcagggagccgtgtgggcgtcgtccaggtggggttttgctattcccttctactggggtattttcttccttgcccccctctcccgtatgaattccagctgaaattcccgtgcgtttcagggaggcggaagcttcgccgtccgggcgccagccggagacggaggaggaggagacgtgggtttctgctcggcatcgttgctctgtttgctcccttggggtgttcgtttcttcctgattgtgttcgtttgcttgcgcggtggtttgcagggagacgaaatttgggactgggttggacgggatgcttaccaggctgcagagggaaagggagagggtccggccgccgctcccacggagcagcgatggagcgggaggggaacgcgacaatgttggtgcgttgggttttcaattcccagcatattcttcagaaacatcaattgttttcaattgtgcatcatatatacttgcttcgcaaaaatagttttacgtgtccctagggaagcattaatctgactacatttgagatgatagcattgatctgactacatttgagatgatagcattaatctgactacatttgagatgattagtccgtgtatcagtgcacttgttgcccccatggttacagcttgtggaatcgtggctggctcctattcagtgcagtactgcagttccttgctggcaaaaagaggcattcatgtcagtttatccaagccgtctgatttttgcggatgacggtcttgtccacaaaccttccctacttctgcctccagtagggaatttcctaaatcaaccattacaattcttcactcttgagtactccctccgtactcataaaggaagtcgtttaggacaatgtttaagttaaaccttgggaatataaatcataaataattctcaagttgttgagtttgaaaatctaaaaattatatgaatagatttgtcttgaaaaatactttcataaaagtatacatatatcacttttcaataaatatttttatagaaacaagacgtcaaagttgtgttttgtagaccgtgtcgctgtcctaaacgacttcctttatgagtatggagggagtacctagtaaaatagttgcttatcagttgtccacatatagctgcacagggggccaaaagttgcctttttcacagtgatgtgtctttctgattttaacaccagttacaatcaagagtaatagtaaaacaaacatcatatgattaaacttcaaatgtagtgtgtgcatgtatgttatattctaagattgaccatgttttaggtattttatcgattatcgcacattcatggtagtcttagttcacttttagtatcatcgaacgacagttatgtcacccactagaaacttataaataggagaagtgaaattgaatgtttgactactgatatcactgattaaatcatgcattgtaattggcaatattgtaaatgaagtaaaacgaaatatgaagtgattatacaccccacaatttacaatattattaatgaacttttggtttgtcgttattggataacttgtttgtacatatttgtggtgttccttttggatgtattatctacttccttttctagtttgtatttgcatataatgtgtataactgaaatcatccttctgtgttgcatatatgcagctttaatgggtcagagtggagcacccagtcactctgcaactggtggcaggtacactccaaaggtacctgataatggaaatatacatagttatcatcctcaaaatggagcactagaacataacaaaagctcaaagagtttgactaatgatgcatggcgaacatggtcccttgacaagggtggtttttcagattttcaaggtcatatgactaactttcttccgtactgcatcccatttgtttgatgattatttgttgacaaggtcaattgttgcagctgctgaaatccattcaactaacagcaggaaatcatttagacatgatggcctagacattcttgcacaagatgacgtgcacgggccatctaatggtgtggctgtacatgattatgatattaatgatgtagactctgaaagagacgatatacatgcacgccttcaaaatttggagttggatcttactgccgctcttcatacattaagatcaagattcgacaaggttatatcagatatggtgtgtatatttcccctaacatcatcaattttgctttcatttgttgtgaattactagtggactataattttttgtgcacgaagtgttttttctgcatctgtaaagctgttagttttacgtaaaaattctgctgccacacaaacaaaagaagaaagatagttccatttatttaattaaacttttgtatgtctaatactccatctgctttatgctctcgggaagtgtgcaataaaaaaatgtattttgagtttttaacttgcagtttcctgattaaaatgcagattagagacgtggaaatgatatgacttgattttccattgaaatttcgttcataatattgtaatttttaactataatgtatatttagcaacacttccatctggcagctacttttgtattgcaatatggcagttacagggcatacaaatacatgaaacactagtggatgaagttgtgctttgtgcctctcaccttgatcttgccagaggcactgtactcattgttctaagcatccaactttgttgtttttttccagtccgaaggtgacggagcaaaagcaccaaatgggctctccgacgattgggaatttgaagagaccaaagtaatgcaagctcaggaagagctgcggtcaatccgtgcaaaaatagcagtattagaaggaaagatggcactggagataatgtatgtccacctaatatctgattacctatgctttaaattttgtatgttcccttttagtaattgtataccatgtcatcagtgagaagaacaaaataattgaagagaaacaaagaaggcttgatgaagctgagaaggccttgagtgagctccgtactgtttatattgtgtggtctaatcctgcttcagaggttctattgactggatcttttgatggctggacaagccaggtgaacatttcaaccagctctcgattacctttcgtaatatttgaacttcgctaaggcttaggatgattgttctgccatgtggtgctaattttgaaagataatatgaaaaataactatcatgcgtctatgtggctagtaggtttagaatcctcatatggatatcattgattttgcccatttaagtatttgaagttcagtgggaatggcttgaacaattcagattgtcctgcacttgcttaaagtagatttggtccagaaggcataactgcatcatacctgactggttctggttttgaatcgtaattggcaattcgatgtaacaagcacttgtatttcatataatataattttaatgttaatgaagccaatctgacactctacatagctctgtacttccatgcttgataacattcgcattgttaccttgttatgcttattattatggggcaattgcaaatttaccactactttgaatcattattgcaaaactgccactagaaaattgcaaaaatgccactagaactgtcattcgagtggtatccttgcaatattcaaaaaaccggtggcaaatttgcaaatgcccctattattatatgttcaaattggaaagttttttaaataagtgttgtgtgtacatttttttatcatctgaaatctgatgataatctaattccataacattgttgactttgtgcagagaagaatggaaagatcagaaagaggcaccttttccttaaacctgaggttgtatcctggtagatatgaggtaatggtctgtcttattatgcttccaggcattattattttcatctcatctgatagcccctctgcctattctccagattaaatttattgttgatggtgtttggaggaatgatcctctgcgcccccttgtgagcaataacgggcatgaaaacaaccttctgactgtcacttgacttgaagctccatttgtcaatcctggtagcatttttgtagattgttatctagatctttatcaacatgattaggccaatccatccagaatggaaacaaaaaaaatgagtgagctggttagctctgttccaccctgtaattatctgcttcgccttaagtcgtcgttctttttctttggtagagattcttacaatttaaacagtgtaagggtgttgtcagaatgcctcaccctgttagataggagaagagaatagttgtgtattcttttcttccccgtcttttgcccggagaagtatagttgtgtgatgggtatccaggtgtctggttgtacaccttcatggaatgtggttgtacatctcttttgtggtgacaaaaactccccccgccggtaactttgcacttgcagaagctttaggttttaaaggtggtcgcctcacttgcgagttgttactacgccttaattacgcaatcttcttatgccagcacagaggatctgatatcacagaggattgaattgtgtgctatgcaagaagagagctctgttgaaattgtgattttctgacgctttgagtatgaaattattgtatctgagaccaggttgaactctga</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057471.2 RefSeq:Os03g0686900]|
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| − | }}
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| − | [[Category:Genes]] | |
| − | [[Category:Japonica mRNA]]
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| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]] | |
| − | [[Category:Japonica Chromosome 3]]
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| − | [[Category:Chromosome 3]]
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