Difference between revisions of "Os01g0878700"
(→Gene Symbol) |
|||
| (6 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
| − | + | The rice '''''Os01g0878700''''' was reported as '''''OsAAP6''''' in 2014 <ref name="ref1" /> by researchers from China. | |
==Annotated Information== | ==Annotated Information== | ||
| + | ===Gene Symbol=== | ||
| + | [[File:Os01g0878700.png|right|thumb|527px|'''Fig. 2.'''Expression patterns of OsAAP6 and subcellular localization analysis.<ref name="ref1" />.'']] | ||
| + | *'''''Os01g0878700''''' '''''<=>''''' '''''OsAAP6''''','''''AAP6''''' | ||
| + | |||
===Function=== | ===Function=== | ||
| − | + | * '''''OsAAP6''''' greatly enhances root absorption of a range of amino acids and has effects on the distribution of various amino acids. | |
| − | + | * '''''OsAAP6''''' encodes a putative amino acid transporter, which functions as a positive regulator of GPC (grain protein content) in rice. | |
===Expression=== | ===Expression=== | ||
| − | + | * Sixteen tissues from the NILs were assayed for temporal and spatial expression patterns of OsAAP6 by quantitative reverse transcription–PCR (Fig. 2a). | |
| + | * OsAAP6 transcripts were detected in all examined tissues and were most abundant in the endosperms at 5–12 days after flowering (DAF) (Fig. 2a), the same timeframe as reported for transcripts of OsAAP6 (ref. 23), and there were significantly higher transcript levels in the endosperms of NIL(ZS97) than in endosperm of NIL(NYZ) (Fig. 2a). | ||
===Evolution=== | ===Evolution=== | ||
| − | + | * To investigate the subcellular localization of OsAAP6 protein, the researchers transiently co-expressed '''''OsAAP6''''' fused to green fluorescent protein (GFP) with various subcellular markers in rice protoplasts 28–31. | |
| + | * Co-expression of '''''OsAAP6-GFP''''' and SCAMP1-RFP clearly showed that '''''OsAAP6''''' was not localized at the plasma membrane or on the trans-Golgi network (Fig. 2n). | ||
| + | * However, '''''OsAAP6'''''-GFP and the endoplasmic reticulum (ER) marker BiP-RFP overlapped completely when co-expressed in rice protoplasts (Fig. 2o). Therefore, '''''OsAAP6''''' is localized on the ER, but not on the plasma membrane or in the trans-Golgi network. | ||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China. | |
| − | + | * Shanghai Agrobiological Gene Center, Shanghai 201106, China. | |
==References== | ==References== | ||
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Peng B, Kong H, Li Y, Wang L, Zhong M, Sun L, Gao G, Zhang Q, Luo L, Wang G, | ||
| + | Xie W, Chen J, Yao W, Peng Y, Lei L, Lian X, Xiao J, Xu C, Li X, He Y. OsAAP6 | ||
| + | functions as an important regulator of grain protein content and nutritional | ||
| + | quality in rice. Nat Commun. 2014 Sep 11;5:4847. doi: 10.1038/ncomms5847. PubMed | ||
| + | PMID: 25209128; PubMed Central PMCID: PMC4175581. | ||
| + | </ref> | ||
| + | </references> | ||
==Structured Information== | ==Structured Information== | ||
| − | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]] | |
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | [[Category:Genes]] | ||
| − | |||
| − | [[Category:Oryza Sativa Japonica Group]] | ||
| − | [[Category:Japonica | ||
| − | |||
| − | |||
Latest revision as of 15:57, 13 October 2016
The rice Os01g0878700 was reported as OsAAP6 in 2014 [1] by researchers from China.
Contents
Annotated Information
Gene Symbol
Fig. 2.Expression patterns of OsAAP6 and subcellular localization analysis.[1].
- Os01g0878700 <=> OsAAP6,AAP6
Function
- OsAAP6 greatly enhances root absorption of a range of amino acids and has effects on the distribution of various amino acids.
- OsAAP6 encodes a putative amino acid transporter, which functions as a positive regulator of GPC (grain protein content) in rice.
Expression
- Sixteen tissues from the NILs were assayed for temporal and spatial expression patterns of OsAAP6 by quantitative reverse transcription–PCR (Fig. 2a).
- OsAAP6 transcripts were detected in all examined tissues and were most abundant in the endosperms at 5–12 days after flowering (DAF) (Fig. 2a), the same timeframe as reported for transcripts of OsAAP6 (ref. 23), and there were significantly higher transcript levels in the endosperms of NIL(ZS97) than in endosperm of NIL(NYZ) (Fig. 2a).
Evolution
- To investigate the subcellular localization of OsAAP6 protein, the researchers transiently co-expressed OsAAP6 fused to green fluorescent protein (GFP) with various subcellular markers in rice protoplasts 28–31.
- Co-expression of OsAAP6-GFP and SCAMP1-RFP clearly showed that OsAAP6 was not localized at the plasma membrane or on the trans-Golgi network (Fig. 2n).
- However, OsAAP6-GFP and the endoplasmic reticulum (ER) marker BiP-RFP overlapped completely when co-expressed in rice protoplasts (Fig. 2o). Therefore, OsAAP6 is localized on the ER, but not on the plasma membrane or in the trans-Golgi network.
You can also add sub-section(s) at will.
Labs working on this gene
- National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Hongshan District, Wuhan 430070, China.
- Shanghai Agrobiological Gene Center, Shanghai 201106, China.
References
- ↑ 1.0 1.1 Peng B, Kong H, Li Y, Wang L, Zhong M, Sun L, Gao G, Zhang Q, Luo L, Wang G, Xie W, Chen J, Yao W, Peng Y, Lei L, Lian X, Xiao J, Xu C, Li X, He Y. OsAAP6 functions as an important regulator of grain protein content and nutritional quality in rice. Nat Commun. 2014 Sep 11;5:4847. doi: 10.1038/ncomms5847. PubMed PMID: 25209128; PubMed Central PMCID: PMC4175581.
