Difference between revisions of "Os03g0300000"
(Created page with "{{JaponicaGene|
GeneName = Os03g0300000|
Description = Similar to Xyloglucan 6-xylosyltransferase (EC 2.4.2.39) (AtXT1)|
Version = NM_001056388.1 GI:115452504 GeneID:433256...") |
(→Phenotypic analysis) |
||
| (8 intermediate revisions by 3 users not shown) | |||
| Line 1: | Line 1: | ||
| − | + | The rice '''''Os03g0300000''''' was reported as '''''OsXXT1''''' in 2014 <ref name="ref1" /> by researchers from China. | |
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | + | ==Annotated Information== | |
| − | + | [[File:67-Os03g0300000.png|right|thumb|327px|'''Figure 1.''' ''Phenotype of root hairs from wild type (WT, cv Kasalath), srh2, and srh2 complemented by Ubiquitin-1 promoter ::OsXXT1.t.<ref name="ref1" />.'']] | |
| − | + | ===Gene Symbol=== | |
| − | + | *'''''Os03g0300000''''' '''<=>''' '''''OsXXT1,XXT1,SRH2,OsSRH2''''' | |
| − | + | ===Function=== | |
| − | + | * '''''OsXXT1''''' in maintaining cell wall structure and tensile strength in rice, a typical grass species that contains relatively low XyG content in cell walls. | |
| − | + | ===Phenotypic analysis=== | |
| − | + | * Seeds from the M 2 generation of an EMS-mutagenized population of Indica cultivar Kasalath, were germinated and grown in nutrient solution to screen for mutants with abnormal root hair phenotype. Seven days after germination, a mutant with significantly reduced length in root hairs was identified (Fig. 1A, B). No obvious difference was observed in leaf or root growth between wild type and mutant (Fig. S1). | |
| − | + | * The mutant was designated as srh2. Scanning electron microscopy of the wild-type and srh2 root surface showed that the length of the root hairs of srh2 were shorter than those of wild type. | |
| − | + | * However, no significant difference was found in root hair density or distribution pattern between srh2 and wild type (Fig. 1D, E). | |
| − | + | ||
| − | + | ===Expression=== | |
| − | + | * '''''OsXXT1''''' and '''''OsGT2''''' have similar expression patterns and are expressed across a variety of tissues. In contrast, other members of this family (OsGT3 to OSGT5) display a more restricted expression pattern, suggesting that '''''OsXXT1''''' and '''''OsGT2''''' are the predominantly expressed genes. | |
| − | + | ||
| − | + | ===Evolution=== | |
| − | + | * '''''OsXXT1''''' displays more than 70% amino acid sequence identity with the previously characterized Arabidopsis thaliana XYG XYLOSYL TRANSFERASE 1 (AtXXT1) and XYG XYLOSYL TRANSFERASE 2 (AtXXT2), which catalyse the transfer of xylose onto β-1,4-glucan chains. | |
| − | + | ||
| − | < | + | You can also add sub-section(s) at will. |
| − | + | ||
| − | + | ==Labs working on this gene== | |
| − | + | * State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, 388 Yuhangtang Road, Hangzhou, 310058, P. R. China | |
| − | + | * University of Western Australia-Zhejiang University Joint Research Laboratory in Genomics and Nutriomics, College of Life Sciences, Zhejiang University, 388 Yuhangtang Road, Hangzhou, 310058, P. R. China | |
| − | + | * ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, Crawley, 6009 Western Australia, Australia | |
| − | [[Category:Genes]] | + | * State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China |
| − | + | * Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe | |
| − | [[Category:Oryza Sativa Japonica Group]] | + | University, Bundara, Victoria 3086, Australia |
| − | [[Category:Japonica | + | |
| − | + | ==References== | |
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Wang C, Li S, Ng S, Zhang B, Zhou Y, Whelan J, Wu P, Shou H. Mutation in | ||
| + | xyloglucan 6-xylosytransferase results in abnormal root hair development in Oryza | ||
| + | sativa. J Exp Bot. 2014 Aug;65(15):4149-57. doi: 10.1093/jxb/eru189. Epub 2014 | ||
| + | May 15. PubMed PMID: 24834920; PubMed Central PMCID: PMC4112626. | ||
| + | </ref> | ||
| + | |||
| + | </references> | ||
| + | |||
| + | ==Structured Information== | ||
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]] | ||
Latest revision as of 06:52, 15 October 2016
The rice Os03g0300000 was reported as OsXXT1 in 2014 [1] by researchers from China.
Contents
Annotated Information
Figure 1. Phenotype of root hairs from wild type (WT, cv Kasalath), srh2, and srh2 complemented by Ubiquitin-1 promoter ::OsXXT1.t.[1].
Gene Symbol
- Os03g0300000 <=> OsXXT1,XXT1,SRH2,OsSRH2
Function
- OsXXT1 in maintaining cell wall structure and tensile strength in rice, a typical grass species that contains relatively low XyG content in cell walls.
Phenotypic analysis
- Seeds from the M 2 generation of an EMS-mutagenized population of Indica cultivar Kasalath, were germinated and grown in nutrient solution to screen for mutants with abnormal root hair phenotype. Seven days after germination, a mutant with significantly reduced length in root hairs was identified (Fig. 1A, B). No obvious difference was observed in leaf or root growth between wild type and mutant (Fig. S1).
- The mutant was designated as srh2. Scanning electron microscopy of the wild-type and srh2 root surface showed that the length of the root hairs of srh2 were shorter than those of wild type.
- However, no significant difference was found in root hair density or distribution pattern between srh2 and wild type (Fig. 1D, E).
Expression
- OsXXT1 and OsGT2 have similar expression patterns and are expressed across a variety of tissues. In contrast, other members of this family (OsGT3 to OSGT5) display a more restricted expression pattern, suggesting that OsXXT1 and OsGT2 are the predominantly expressed genes.
Evolution
- OsXXT1 displays more than 70% amino acid sequence identity with the previously characterized Arabidopsis thaliana XYG XYLOSYL TRANSFERASE 1 (AtXXT1) and XYG XYLOSYL TRANSFERASE 2 (AtXXT2), which catalyse the transfer of xylose onto β-1,4-glucan chains.
You can also add sub-section(s) at will.
Labs working on this gene
- State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, 388 Yuhangtang Road, Hangzhou, 310058, P. R. China
- University of Western Australia-Zhejiang University Joint Research Laboratory in Genomics and Nutriomics, College of Life Sciences, Zhejiang University, 388 Yuhangtang Road, Hangzhou, 310058, P. R. China
- ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, Crawley, 6009 Western Australia, Australia
- State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
- Department of Botany, School of Life Science, Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe
University, Bundara, Victoria 3086, Australia
References
- ↑ 1.0 1.1 Wang C, Li S, Ng S, Zhang B, Zhou Y, Whelan J, Wu P, Shou H. Mutation in xyloglucan 6-xylosytransferase results in abnormal root hair development in Oryza sativa. J Exp Bot. 2014 Aug;65(15):4149-57. doi: 10.1093/jxb/eru189. Epub 2014 May 15. PubMed PMID: 24834920; PubMed Central PMCID: PMC4112626.
