Difference between revisions of "Os06g0325200"

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Please input one-sentence summary here.
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The rice '''''Os06g0325200''''' was reported as '''''OsPT10''''' in 2014 <ref name="ref1" /> by researchers from China.  
  
 
==Annotated Information==
 
==Annotated Information==
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[[File:78-Os06g0324800.png|right|thumb|427px|'''Figure 1.''' ''Tissue expression pattern of OsPT9 and OsPT10 under phosphate (Pi)-sufficient (+P) and Pi-starvation (−P) conditions.<ref name="ref1" />.'']]
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===Gene Symbol===
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*'''''Os06g0324800''''' '''''<=>''''' '''''PT10''''','''''OsPT10,OsPHT1;10'''''
 
===Function===
 
===Function===
Please input function information here.
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* High-affinity Km values for Pi transport of '''''OsPT9''''' and '''''OsPT10''''' were determined by yeast experiments and two-electrode voltage clamp analysis of anion transport in Xenopus oocytes expressing '''''OsPT9''''' and '''''OsPT10'''''.
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* '''''OsPT9''''' and '''''OsPT10''''' are high-affinity Pi transporters.
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* '''''OsPT9''''' and '''''OsPT10''''' redundantly function in Pi uptake.
  
 
===Expression===
 
===Expression===
Please input expression information here.
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* Semi-quantitative RT-PCR analysis of seedlings grown in solution cultures under Pi-sufficient (+P) or Pi-starvation (–P) conditions as well as starvation of other essential nutrients showed that the expression of '''''OsPT9''''' and '''''OsPT10''''' in both roots and leaves was specifically induced by Pi starvation
 
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* The GUS staining analysis showed expression of '''''OsPT9''''' in both roots and leaves under Pi starvation (–P). In contrast, no GUS staining was observed under either Pi-sufficient (+P) or –P conditions for '''''OsPT10''''' (Fig. 1b).
===Evolution===
 
Please input evolution information here.
 
  
 
You can also add sub-section(s) at will.
 
You can also add sub-section(s) at will.
  
 
==Labs working on this gene==
 
==Labs working on this gene==
Please input related labs here.
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* State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058,
 
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China
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* The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Agriculture and Food Science, Zhejiang A & F University, Lin’an 311300, China
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* Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY 14853, USA
 
==References==
 
==References==
Please input cited references here.
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<references>
 
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* <ref name="ref1">
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Wang X, Wang Y, Piñeros MA, Wang Z, Wang W, Li C, Wu Z, Kochian LV, Wu P.
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Phosphate transporters OsPHT1;9 and OsPHT1;10 are involved in phosphate uptake in
 +
rice. Plant Cell Environ. 2014 May;37(5):1159-70. doi: 10.1111/pce.12224. Epub
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2013 Dec 17. PubMed PMID: 24344809.
 +
</ref>
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</references>
 
==Structured Information==
 
==Structured Information==
{{JaponicaGene|
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    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 6]]
GeneName = Os06g0325200|
 
Description = Major facilitator superfamily protein|
 
Version = NM_001187832.1 GI:297724794 GeneID:9267349|
 
Length = 2545 bp|
 
Definition = Oryza sativa Japonica Group Os06g0325200, complete gene.|
 
Source = Oryza sativa Japonica Group
 
 
 
  ORGANISM  Oryza sativa Japonica Group
 
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
 
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
 
            clade; Ehrhartoideae; Oryzeae; Oryza.
 
|
 
Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
 
AP = Chromosome 6:12760306..12762850|
 
CDS = 12760306..12761266,12762153..12762850|
 
GCID = <gbrowseImage1>
 
name=NC_008399:12760306..12762850
 
source=RiceChromosome06
 
preset=GeneLocation
 
</gbrowseImage1>|
 
GSID = <gbrowseImage2>
 
name=NC_008399:12760306..12762850
 
source=RiceChromosome06
 
preset=GeneLocation
 
</gbrowseImage2>|
 
CDNA = <cdnaseq>atggctccgatcggcgtgctcacggcgctggaccaggcgcggacgcagtactaccacttcaaggccatcgtcatcgccggcatgggactcttcaccgactcctacgacctcttctgcatcgcgcccgtcatgaagatcgtgggccgggtgtactactccgacggcggcgcccgtccgggggtgacgccgccggccgtcgtgtcggcgaccgtcggagtggcgctgctgggcgcggtgatcgggaacgtggtgttcggcgcgctgggcgaccgcgtggggcggcgccgcgtctacggcgcgtgcctgctgctgatggtgtgcagctccgtcgggagcggcttctccgtgtgccggacgcgccggtgcgcgctcgccagcctctgcttcttccgcttcctcctcggcgtcggcgtcggcggcgactacccgctgtcggccaccatcatgtcggagttcgccaaccggcgcacccgcggcgcgttcatcgccgccgtgttctccatgcaggggttcggcatcctcgccagctccgcggtcaccatggccgtcgccgccgccttcgaccactacaccggctaccccgcgccgctcgacacgccggagtgcgccgacctcgcctggcggatcattctcatggccggcgccgtccccgccgcgctgacgtactactggaggatgtccatgccggagacggccagatatacggctttggtggagcgcgacgtggtgaaggccaccaacgacatcggccgcgttctggccgacctcgacctgggcgccgtcgccgaggaggaggtggcggcggcgttgagccggcctccgccgccgccgcgcccttcgtacggcctcctctctcggcgcttcgtccggcagcacgggcgggacctgttcgcgtgcgcggcggcgtggttcctcctcgacatcccctactacagcagcacgctgttccagtctcagatctaccggccgctgttcccggcgccggggctcatcaacgcgttccaggaggcgttcaacgtggccaagttccaggccgtcatcgccgtcgcctccaccatcccgggctacttcgtcgccgtgctcctcatcgaccgcgtcggccggcggtgcctccagatggccgggttcctcctcatggccgtcttcctcttcgcgctcgcgggtccgtacgacggatactggcgcgaccacggcgcgcacgccggctacatcgtgctctactcgctcaccttcttctccgccaacctcgggccgaacaccaccaccttcatcctgccggcggagctcttcccggcgcgcttccggtcgacgtgccacggcctctccggcgcggcggggaagctcggcgcgctcgtcggctccatcggcttcctgtgggcgtcgcagcagaaggacggcgcggcggccgggcacctgccgggcatcggcatgatgtacgcgctgttcgttcttggcgggatctgcctgctcgggctggcactcacctacgtgttcacgccggagacgatgatgcggtcgctggaggagaacgagagcgacagggcgcagacccaggtcggcgacggcggcagcgacaccgaggcggccaagagcccggcgtccatggcgagctcgcacctcagcatgtcgcccatccttccagcccgggtctccgtgtga</cdnaseq>|
 
AA = <aaseq>MAPIGVLTALDQARTQYYHFKAIVIAGMGLFTDSYDLFCIAPVM                    KIVGRVYYSDGGARPGVTPPAVVSATVGVALLGAVIGNVVFGALGDRVGRRRVYGACL                    LLMVCSSVGSGFSVCRTRRCALASLCFFRFLLGVGVGGDYPLSATIMSEFANRRTRGA                    FIAAVFSMQGFGILASSAVTMAVAAAFDHYTGYPAPLDTPECADLAWRIILMAGAVPA                    ALTYYWRMSMPETARYTALVERDVVKATNDIGRVLADLDLGAVAEEEVAAALSRPPPP                    PRPSYGLLSRRFVRQHGRDLFACAAAWFLLDIPYYSSTLFQSQIYRPLFPAPGLINAF                    QEAFNVAKFQAVIAVASTIPGYFVAVLLIDRVGRRCLQMAGFLLMAVFLFALAGPYDG                    YWRDHGAHAGYIVLYSLTFFSANLGPNTTTFILPAELFPARFRSTCHGLSGAAGKLGA                    LVGSIGFLWASQQKDGAAAGHLPGIGMMYALFVLGGICLLGLALTYVFTPETMMRSLE                    ENESDRAQTQVGDGGSDTEAAKSPASMASSHLSMSPILPARVSV</aaseq>|
 
DNA = <dnaseqindica>1585..2545#1..698#atggctccgatcggcgtgctcacggcgctggaccaggcgcggacgcagtactaccacttcaaggccatcgtcatcgccggcatgggactcttcaccgactcctacgacctcttctgcatcgcgcccgtcatgaagatcgtgggccgggtgtactactccgacggcggcgcccgtccgggggtgacgccgccggccgtcgtgtcggcgaccgtcggagtggcgctgctgggcgcggtgatcgggaacgtggtgttcggcgcgctgggcgaccgcgtggggcggcgccgcgtctacggcgcgtgcctgctgctgatggtgtgcagctccgtcgggagcggcttctccgtgtgccggacgcgccggtgcgcgctcgccagcctctgcttcttccgcttcctcctcggcgtcggcgtcggcggcgactacccgctgtcggccaccatcatgtcggagttcgccaaccggcgcacccgcggcgcgttcatcgccgccgtgttctccatgcaggggttcggcatcctcgccagctccgcggtcaccatggccgtcgccgccgccttcgaccactacaccggctaccccgcgccgctcgacacgccggagtgcgccgacctcgcctggcggatcattctcatggccggcgccgtccccgccgcgctgacgtactactggaggatgtccatgccggagacggccaggtaccaactatacttgggccgctgacatgtggtcccagatgtcattcatgcattggcttcctccgtacctttgacttcacagcaaccatttgaaccttaaaaaaatcttaaataatagtatttgattggtaaattagattaagtgtacatggaccgtgttaaacaaatgtgatagataaggaatataccaaacgagattccaagaacaaattctgagaggaaaatttatccttgataagtatgctaattgcctggcactggcagctccatggtcaattaagcttccttctcttcgttgatgccgaaaatttcaaatggatagtttagtcggaatattaaataattgggcatgtttatcgtcactgccatcgttaattagcactagtacttatacattatatatcccaaaagaaaatgtctacacatacgaatagcgtatacgatttgtgttcgactagtgcaaagctgctacatgcctagacccacatggaaagatattgagagtgttgatcggtggtcctagacccacatggaaagatgttgagagtgttgattggtggtcggatgtgagtcgtacagctccagtcagacgtatagcagaactcatcacgaaattgtctctagctactctttgatttgctaggtgtactgtcattgctgatgtcaggtctataaatatgattttgatttttgattagtggcacagtgtaattgcacatttaacccctttgcactctaatcaataaaattgccctttaactttatgctgaaagttgctataagtgagtagattaattacaaaaaagggaattaagcacgtactaatggttaatgacgaaaaagtaatgaccgtgcataacgttgtatttgtgctgccaacgagcagatatacggctttggtggagcgcgacgtggtgaaggccaccaacgacatcggccgcgttctggccgacctcgacctgggcgccgtcgccgaggaggaggtggcggcggcgttgagccggcctccgccgccgccgcgcccttcgtacggcctcctctctcggcgcttcgtccggcagcacgggcgggacctgttcgcgtgcgcggcggcgtggttcctcctcgacatcccctactacagcagcacgctgttccagtctcagatctaccggccgctgttcccggcgccggggctcatcaacgcgttccaggaggcgttcaacgtggccaagttccaggccgtcatcgccgtcgcctccaccatcccgggctacttcgtcgccgtgctcctcatcgaccgcgtcggccggcggtgcctccagatggccgggttcctcctcatggccgtcttcctcttcgcgctcgcgggtccgtacgacggatactggcgcgaccacggcgcgcacgccggctacatcgtgctctactcgctcaccttcttctccgccaacctcgggccgaacaccaccaccttcatcctgccggcggagctcttcccggcgcgcttccggtcgacgtgccacggcctctccggcgcggcggggaagctcggcgcgctcgtcggctccatcggcttcctgtgggcgtcgcagcagaaggacggcgcggcggccgggcacctgccgggcatcggcatgatgtacgcgctgttcgttcttggcgggatctgcctgctcgggctggcactcacctacgtgttcacgccggagacgatgatgcggtcgctggaggagaacgagagcgacagggcgcagacccaggtcggcgacggcggcagcgacaccgaggcggccaagagcccggcgtccatggcgagctcgcacctcagcatgtcgcccatccttccagcccgggtctccgtgtga</dnaseqindica>|
 
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001187832.1 RefSeq:Os06g0325200]|
 
}}
 
[[Category:Genes]]
 
[[Category:Japonica mRNA]]
 
[[Category:Oryza Sativa Japonica Group]]
 
[[Category:Japonica Genes]]
 
[[Category:Japonica Chromosome 6]]
 
[[Category:Chromosome 6]]
 

Latest revision as of 09:28, 15 October 2016

The rice Os06g0325200 was reported as OsPT10 in 2014 [1] by researchers from China.

Annotated Information

Figure 1. Tissue expression pattern of OsPT9 and OsPT10 under phosphate (Pi)-sufficient (+P) and Pi-starvation (−P) conditions.[1].

Gene Symbol

  • Os06g0324800 <=> PT10,OsPT10,OsPHT1;10

Function

  • High-affinity Km values for Pi transport of OsPT9 and OsPT10 were determined by yeast experiments and two-electrode voltage clamp analysis of anion transport in Xenopus oocytes expressing OsPT9 and OsPT10.
  • OsPT9 and OsPT10 are high-affinity Pi transporters.
  • OsPT9 and OsPT10 redundantly function in Pi uptake.

Expression

  • Semi-quantitative RT-PCR analysis of seedlings grown in solution cultures under Pi-sufficient (+P) or Pi-starvation (–P) conditions as well as starvation of other essential nutrients showed that the expression of OsPT9 and OsPT10 in both roots and leaves was specifically induced by Pi starvation
  • The GUS staining analysis showed expression of OsPT9 in both roots and leaves under Pi starvation (–P). In contrast, no GUS staining was observed under either Pi-sufficient (+P) or –P conditions for OsPT10 (Fig. 1b).

You can also add sub-section(s) at will.

Labs working on this gene

  • State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058,

China

  • The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Agriculture and Food Science, Zhejiang A & F University, Lin’an 311300, China
  • Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY 14853, USA

References

  1. 1.0 1.1 Wang X, Wang Y, Piñeros MA, Wang Z, Wang W, Li C, Wu Z, Kochian LV, Wu P. Phosphate transporters OsPHT1;9 and OsPHT1;10 are involved in phosphate uptake in rice. Plant Cell Environ. 2014 May;37(5):1159-70. doi: 10.1111/pce.12224. Epub 2013 Dec 17. PubMed PMID: 24344809.

Structured Information