Difference between revisions of "Os08g0191700"
(Created page with "{{JaponicaGene|
GeneName = Os08g0191700|
Description = Similar to Lactoylglutathione lyase (EC 4.4.1.5) (Methylglyoxalase) (Aldoketomutase) (Glyoxalase I) (Glx I) (Ketone-al...") |
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| − | + | The rice '''''Os08g0191700''''' was reported as '''''OsGLYI-11.2''''' in 2014 <ref name="ref1" /> by researchers from India. | |
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| − | + | ==Annotated Information== | |
| − | + | [[File:96-Os08g0191700.png|right|thumb|327px|'''Figure 5.''' ''Germination and growth assay of OsGLYI-11.2 transgenic tobacco under various abiotic stress conditions.<ref name="ref1" />.'']] | |
| − | + | ===Gene Symbol=== | |
| − | + | *'''''Os08g0191700''''' '''''<=>''''' '''''OsGLYI-11.2,OsGLYI11,GLYI11''''' | |
| − | + | ===Function=== | |
| − | + | * '''''OsGLYI-11.2''''' requires Ni2+ for its activity. Its biochemical, structural and functional characterization revealed it to be a monomeric enzyme, possessing a single Ni 2+ coordination site despite containing two GLY I domains. | |
| − | + | ||
| − | + | ===Phenotypic analysis=== | |
| − | + | * Heterologous expression of '''''OsGLYI-11.2''''' in Escherichia coli and model plant Nicotiana tabacum (tobacco) resulted in improved adaptation to various abiotic stresses caused by increased scavenging of MG, lower Na + /K + ratio and maintenance of reduced glutathione levels. | |
| − | + | * Seeds from WT and OsGLYI-11.2 transgenic plants (line 7.1) showed comparable germination under non-stress conditions (Figure 5a, panel 1); however, under osmotic, MG, oxidative and salinity stress, the germination and growth rate of WT reduced significantly when compared with the transgenic line (Figure 5a, panels 2–4). | |
| − | + | * Under stress conditions, the shoot and the root lengths of the WT were severely affected, whereas the transgenic plants showed healthier leaves and better root growth (Figure 5b,c). Furthermore, to score visual stress-induced injury, we calculated the stress tolerance index (STI%) based on their ability to grow under different stress conditions as compared with the non-stressed condition. | |
| − | + | * STI was measured in terms of total fresh weight of both shoots and roots. The negative effect on growth was much higher in WT seedlings as compared with the OsGLYI-11.2 transgenic seedlings under all the stresses in terms of STI (Figure 5d). | |
| − | + | ===Expression=== | |
| − | + | * The expression of '''''OsGLYI-11.2''''' was found to be highly substrate inducible, suggesting an important mode of regulation for its cellular levels. | |
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| − | + | You can also add sub-section(s) at will. | |
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| − | < | + | ==Labs working on this gene== |
| − | + | * Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India | |
| − | + | * Structural and Computational Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India | |
| − | + | * School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India | |
| − | + | * Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India | |
| − | + | ==References== | |
| − | [[Category:Genes]] | + | <references> |
| − | + | * <ref name="ref1"> | |
| − | [[Category:Oryza Sativa Japonica Group]] | + | Mustafiz A, Ghosh A, Tripathi AK, Kaur C, Ganguly AK, Bhavesh NS, Tripathi JK, |
| − | + | Pareek A, Sopory SK, Singla-Pareek SL. A unique Ni2+ -dependent and | |
| − | [[Category:Japonica | + | methylglyoxal-inducible rice glyoxalase I possesses a single active site and |
| − | + | functions in abiotic stress response. Plant J. 2014 Jun;78(6):951-63. doi: | |
| + | 10.1111/tpj.12521. Epub 2014 May 23. PubMed PMID: 24661284. | ||
| + | </ref> | ||
| + | </references> | ||
| + | |||
| + | ==Structured Information== | ||
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 8]] | ||
Latest revision as of 04:33, 17 October 2016
The rice Os08g0191700 was reported as OsGLYI-11.2 in 2014 [1] by researchers from India.
Contents
Annotated Information
Figure 5. Germination and growth assay of OsGLYI-11.2 transgenic tobacco under various abiotic stress conditions.[1].
Gene Symbol
- Os08g0191700 <=> OsGLYI-11.2,OsGLYI11,GLYI11
Function
- OsGLYI-11.2 requires Ni2+ for its activity. Its biochemical, structural and functional characterization revealed it to be a monomeric enzyme, possessing a single Ni 2+ coordination site despite containing two GLY I domains.
Phenotypic analysis
- Heterologous expression of OsGLYI-11.2 in Escherichia coli and model plant Nicotiana tabacum (tobacco) resulted in improved adaptation to various abiotic stresses caused by increased scavenging of MG, lower Na + /K + ratio and maintenance of reduced glutathione levels.
- Seeds from WT and OsGLYI-11.2 transgenic plants (line 7.1) showed comparable germination under non-stress conditions (Figure 5a, panel 1); however, under osmotic, MG, oxidative and salinity stress, the germination and growth rate of WT reduced significantly when compared with the transgenic line (Figure 5a, panels 2–4).
- Under stress conditions, the shoot and the root lengths of the WT were severely affected, whereas the transgenic plants showed healthier leaves and better root growth (Figure 5b,c). Furthermore, to score visual stress-induced injury, we calculated the stress tolerance index (STI%) based on their ability to grow under different stress conditions as compared with the non-stressed condition.
- STI was measured in terms of total fresh weight of both shoots and roots. The negative effect on growth was much higher in WT seedlings as compared with the OsGLYI-11.2 transgenic seedlings under all the stresses in terms of STI (Figure 5d).
Expression
- The expression of OsGLYI-11.2 was found to be highly substrate inducible, suggesting an important mode of regulation for its cellular levels.
You can also add sub-section(s) at will.
Labs working on this gene
- Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
- Structural and Computational Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
- School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India
- Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India
References
- ↑ 1.0 1.1 Mustafiz A, Ghosh A, Tripathi AK, Kaur C, Ganguly AK, Bhavesh NS, Tripathi JK, Pareek A, Sopory SK, Singla-Pareek SL. A unique Ni2+ -dependent and methylglyoxal-inducible rice glyoxalase I possesses a single active site and functions in abiotic stress response. Plant J. 2014 Jun;78(6):951-63. doi: 10.1111/tpj.12521. Epub 2014 May 23. PubMed PMID: 24661284.
