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| − | Please input one-sentence summary here.
| + | The rice '''''Os01g0633100''''' was reported as '''''OsAGPL2''''' in 2014 <ref name="ref1" /> by researchers from the USA and Japan. |
| | | | |
| | ==Annotated Information== | | ==Annotated Information== |
| | + | [[File:106-Os01g0633100.png|right|thumb|327px|'''Figure 1.''' ''Morphology of wild-type, shr1, shr1a and shr2 de-hulled seeds. A cross-section of each seed is also shown..<ref name="ref1" />.'']] |
| | + | ===Gene Symbol=== |
| | + | *'''''Os01g0633100''''' '''''<=>''''' '''''OsAGPase''''','''''AGPase''''' |
| | ===Function=== | | ===Function=== |
| − | Please input function information here.
| + | * L2 subunit of cytosolic isoform of ADPglucose pyrophosphorylase (AGPase) encoded by '''''OsAGPL2''''' is essential for both catalysis and allosteric regulatory properties of the heterotetramer enzyme. |
| | + | * Missense and nonsense mutations in the '''''OsAGPL2''''' gene result in similar decreases in seed weight |
| | | | |
| − | ===Expression=== | + | ===Phenotypic analysis=== |
| − | Please input expression information here.
| + | * The researchers isolated several allelic missense and nonsense '''''OsAGPL2''''' mutants by N-methyl-N-nitrosourea (MNU) treatment of fertilized egg cells and by TILLING (Targeting Induced Local Lesions in Genomes). |
| − | | + | * Interestingly, seeds from three of the missense mutants (two containing T139I and A171V) were severely shriveled and had seed weight and starch content comparable with the shriveled seeds from '''''OsAGPL2''''' null mutants. |
| − | ===Evolution===
| + | * Results from kinetic analysis of the purified recombinant enzymes revealed that the catalytic and allosteric regulatory properties of these mutant enzymes were significantly impaired. |
| − | Please input evolution information here.
| + | * The missense heterotetramer enzymes and the S2b homotetramer had lower specific (catalytic) activities and affinities for the activator 3-phosphoglycerate (3-PGA). The missense heterotetramer enzymes showed more sensitivity to inhibition by the inhibitor inorganic phosphate (Pi) than the wild-type AGPase, while the S2b homotetramer was profoundly tolerant to P i inhibition. |
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| | You can also add sub-section(s) at will. | | You can also add sub-section(s) at will. |
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| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | Please input related labs here.
| + | * Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, USA |
| | + | * Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581 Japan |
| | + | * Department of Genetic Resources Technology, Kyushu University, Fukuoka, 812-8581 Japan |
| | + | * Kazusa DNA Research Institute, Department of Plant Genome Research, Kisarazu, Japan |
| | + | * Faculty of Bioresource Sciences, Akita Prefectural University, Akita City, 010-0195 Japan |
| | | | |
| | ==References== | | ==References== |
| − | Please input cited references here.
| + | <references> |
| | + | * <ref name="ref1"> |
| | + | Tuncel A, Kawaguchi J, Ihara Y, Matsusaka H, Nishi A, Nakamura T, Kuhara S, |
| | + | Hirakawa H, Nakamura Y, Cakir B, Nagamine A, Okita TW, Hwang SK, Satoh H. The |
| | + | rice endosperm ADP-glucose pyrophosphorylase large subunit is essential for |
| | + | optimal catalysis and allosteric regulation of the heterotetrameric enzyme. Plant |
| | + | Cell Physiol. 2014 Jun;55(6):1169-83. doi: 10.1093/pcp/pcu057. Epub 2014 Apr 18. |
| | + | PubMed PMID: 24747952. |
| | + | </ref> |
| | + | </references> |
| | | | |
| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]] |
| − | GeneName = Os01g0633100|
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| − | Description = Similar to ADP-glucose pyrophosphorylase subunit SH2|
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| − | Version = NM_001050189.1 GI:115438748 GeneID:4326594|
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| − | Length = 8079 bp|
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| − | Definition = Oryza sativa Japonica Group Os01g0633100, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| − | | |
| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
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| − | AP = Chromosome 1:27010651..27018729|
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| − | CDS = 27014443..27014649,27015042..27015164,27015770..27015940,27016038..27016127,27016232..27016318<br>,27016399..27016454,27016547..27016640,27016767..27016879,27016946..27017018<br>,27017338..27017418,27017600..27017686,27017766..27017870,27018014..27018120<br>,27018216..27018276,27018369..27018470|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008394:27010651..27018729
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| − | source=RiceChromosome01
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008394:27010651..27018729
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| − | source=RiceChromosome01
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>atgcaattcatgatgccattggatacgaatgcttgtgcacaaccaatgagaagggctggtgagggtgctgggactgagaggttgatggaaagattgaatattgggggcatgactcaggagaaagcactgaggaagaggtgctttggtgatggagttactggaactgcacgatgtgtgttcacatcagatgctgatagagacactcctcatcttcggacacaatcctcccgaaagaattatgctgatgcaagccacgtttctgctgtcattttgggtggaggcactggagttcaactctttcctctgacaagcacaagggctacccccgctgttcctgttggaggatgttacaggcttattgacatccctatgagcaattgcttcaatagcggaataaataaaatatttgtgatgactcagttcaattctgcttctcttaatcgccatatccatcatacataccttggtggggggatcaactttactgatgggtctgtgcaggtattggctgctacacaaatgcctgacgaaccggctggatggttccagggtacagcagatgctatcagaaaatttatgtggatacttgaggatcattacaatcaaaacaacattgagcacgttgtaatcttgtgtggagatcagctttacaggatgaattacatggagcttgtgcagaaacatgtcgatgacaatgctgacattaccatatcatgtgctcccatcgacgggagccgagcttctgactatggactggtgaagtttgatgattcaggccgtgtgatccaatttcttgagaaaccagagggtgctgatttggaatcgatgaaagttgataccagcttcctcagctatgcaatagatgacaaacaaaagtatccctacattgcatcaatgggaatttatgtcttgaaaaaggatgttcttttagacattctgaagtcaaaatatgcacacctgcaagactttggatcagaaattctcccgagagctgttttagagcataatgtgaaggcatgtgtcttcacagaatattgggaggatattggaacaatcaaatcattttttgatgctaacttggccctcaccgagcagcctccaaagttcgaattttatgatccaaaaactcctttcttcacctcacctcgatacttacctccagcaagattggagaagtgcaagatcaaagatgcaataatctccgatggctgttcctttagtgaatgcaccattgagcattctgtcattggaatctcttcacgcgttagcatcgggtgtgaactcaaggataccatgatgatgggtgcagatcaatatgaaactgaagaagaaacttcaaagctattgtttgaaggcaaggtcccaattggtataggcgagaacacaaagataaggaactgtatcatcgacatgaatgctagaattggcaggaacgtgatcattgcaaacactcagggggtccaagagtctgatcaccctgaagagggatactacataaggtctggaattgtggtgatcctgaagaatgcaaccatcaaggacgggaccgtcatataa</cdnaseq>|
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| − | AA = <aaseq>MQFMMPLDTNACAQPMRRAGEGAGTERLMERLNIGGMTQEKALR KRCFGDGVTGTARCVFTSDADRDTPHLRTQSSRKNYADASHVSAVILGGGTGVQLFPL TSTRATPAVPVGGCYRLIDIPMSNCFNSGINKIFVMTQFNSASLNRHIHHTYLGGGIN FTDGSVQVLAATQMPDEPAGWFQGTADAIRKFMWILEDHYNQNNIEHVVILCGDQLYR MNYMELVQKHVDDNADITISCAPIDGSRASDYGLVKFDDSGRVIQFLEKPEGADLESM KVDTSFLSYAIDDKQKYPYIASMGIYVLKKDVLLDILKSKYAHLQDFGSEILPRAVLE HNVKACVFTEYWEDIGTIKSFFDANLALTEQPPKFEFYDPKTPFFTSPRYLPPARLEK CKIKDAIISDGCSFSECTIEHSVIGISSRVSIGCELKDTMMMGADQYETEEETSKLLF EGKVPIGIGENTKIRNCIIDMNARIGRNVIIANTQGVQESDHPEEGYYIRSGIVVILK NATIKDGTVI</aaseq>|
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| − | DNA = <dnaseqindica>3793..3999#4392..4514#5120..5290#5388..5477#5582..5668#5749..5804#5897..5990#6117..6229#6296..6368#6688..6768#6950..7036#7116..7220#7364..7470#7566..7626#7719..7820#aattcctgacggcttcacccacgcttcccccggaaggccggaaatgctccgccgggacagcagagacggacatcgccggtgcggtggctccggcgccggcgccggcgggagcgctccccaccgtgaaaaccctagcgctcctgcgtccagtccccagcggtccgcgagaagccatcgacgcgacgcggagcctgatggtgatggacccgtcgtcgcttcctcggtcgccccgatttcctcccccgcggcaccgctgccgggttccggtccccggtggcgacgctcccggattggcttatacatccggaggccaatcctgcacgggatgccctgtaccaccatctcgtcctccgcttcaccgtctcggcgacacgctcggtgccactaactccctgtttccctaagtactcaattactcatatcattctcatcattaatgtcccttctgcaacaaaatggtaagctatgttttggacacattcccattcatttccctagtacagcggtggtggttcttttgagtttgcatatgatggaggggataggtttgcgttgcgttcatccttatgtatccaatattaagataagtgtagcatcggtagcacaatgtttccagaattcttttatatgcaaagagttcgtgtcaaacaagtaggcatgtttcaactggattgaagcatacaaccatatatatatatatatatatatatataacaaagttcaaaaggattgcattgattgaacaaagagataaccaattatttcctttttttttcttttgtatgttgtatggtgtttgctgaaaaggatcaaaacctgaacagtataactcaaggtttaatggaggagcaagaaatgtactaatatgacaccttataatagtttgtttggacaaaaggagaaggggattatgctcaatcccttcggcttggcagagaaacaaatcctgacagaataaaaagaacaagtttctatattttagattacgatagtttgacctgtacttttttccttcaagtgaagtgctatctcaaagttgtaaaaaaatagtttcttcaatttttcagttatatttattttgaaaatgggaagttacaatatctttttgatttaaatggtcttttccttcagaactggaccctcttacagaattgactagcagttaatttacctgtgcaaatgcttggatcaatgactagttactttcaaaagtcataagaacggtctctctttcagtatttgacggtgtgttgcttgttgaacatatagccatagccatgtattggtgtacaatgcaaattcatgttttataggcaagaaaactggactgccttggagagatgagttcaaaggtacaacattctaagttcttctgtttatgagaataatgtttacaatatgttaagattcagaattgccttgtgaaactcctaaatatatttttactgaacatggaactacaagtgttatattgcacagtaaaatacattaaacaaatttaataatatacttttgtactaataaatatttttcatattatatcaagactttactcgagttttctcggtcccatcattggttctctgaagcatggttcatctctattttttttagcatcataagaaatcggataagattgagctcatatttcacaaatgcgaatcaattttactgtaaaatagaactcaaatgaactgagactggagagcaagatagaagtggcaacatatggaaattgaaaagtcattttggtatttatctgaaagctttattgagctttgtgaatggaccttgcgtgagctctgaaaccagcttaccaccgttagttccagctcggttttctttttttccccttaaaggtttcatgcgtatcgtgacagatgttacataatgacaaattccccagctggagcacctttatccctgctgtttgcatgaaattagcttgtcttgtagttccctccagcaaaaagaagtctgaaacaaaacaacatttcgaaaaaaaggcatccatgagttagcatttctacagttgtctatagaggggaaggctgcacgacaaagtttccaggcttggaaacaacctcttatgtaaaatttttcgtatgtatcagatgatttgtttgcgttacggcatctccacctaacatcaccttcatcatgcgcctatggtctttctcttgcctgttttatacgtaaaattggaaacgacagaaacttttgccatctttattaaaggaaggcaaatatgcaaatataggcatcaagatcacagttagtggattatcatctttgtaggttaacatgtcctaccccaggggagcttatactcaagtactccatgcattttcatgaaatgagaaaaaacgatttttaagagaaatgtactttcttgtatttatgccaaatggcaaggactgaaagggaaaaactaagaaagggaacgttacagtaaggctctgtggggactggggacttcagagaaacgtgaaccctgcttccttcctctgcatgaacataacaccagaggtttccagcctttcacacagttgttgatggcttcacacaattcatctctacctcctgactctttataaggacccccagcatcaccacaattgcacaagtacaggcattagatccacaagaacacttgggcaggcaagcacctctttgatctttaagccgttgttatgttctatttctgagcatatggtttctagttatattctttttcttcattcgtttcatatctttgaagtgttgatgcaaatgcggtgaacaactatcaactgtgtactctccaagtgaatgcgaataatcatttcctgtgagaattgtgggctagataaacgaatgaaatgctgttttatctatgtcatgtgtggaaatttagttaattttccggtctttttatgcattgagatgggtatgctgtttttttagttgggtcccatcatcttgagaattctttcaaatttccttttctttatcctatataaaggatagagaaggcgtatgcctaggtgcaccaaccctgaaagttttattctaattgcgggaatggtttgtaatttttgcttgttcaggttctttttcgtggcctttcttttttttccccttattttgcttagtctttcacagtccaatttttgggaagtagtatatcttagtttggtcctaaggcaccatgttgtactgcaggaaaaaaaagagtaattgtattctgttttttccttgattactatatccctgttttaattaattttgtgcctttgttgtttgatgttggaacttcaatgcccataattagtcatttgacttgttttgggttttgacgctatcttgagtgccataggaaactggtagaatttagtaataattttatatagactgaatgttgagcccaccacaaatggtttccttctgtacaagtatttaataactcaagcacaggaaacatcagatctctaatctaaaggttaacaatgggctcaagcaggagcagtagttcagctctatctgtatatttagaagggctggatctacctgtccaccagcttttaattttaccctggcagctggataacttcttgtctgttaatttcatttagtgctgtgttattttcttcttgttgttcaggatggatgcttttgaatttctggaatttcgtattttgttctatctctttatgaaatgacgttatggcacactttttctgcatattcttgatgaaaataattacctagtcatttttttagttgcaggtttgtctgggactttgagtacccatgcaattcatgatgccattggatacgaatgcttgtgcacaaccaatgagaagggctggtgagggtgctgggactgagaggttgatggaaagattgaatattgggggcatgactcaggagaaagcactgaggaagaggtgctttggtgatggagttactggaactgcacgatgtgtgttcacatcagatgctgatagagacactcctgtaagcatttaactctcttgtaaatttgtcgttttctttttattgaatttgttatttcacaattgtgttccacaggcacaatgcacttattcaagattatagagaattgtagctttcatcactgcattcgacctatggacatcttgatttactaataatacagtaagaacttatgggacaaattttataagatggttaaccagccagaagattggataattgatgtggtgtagttccatgcaatatacaattaattatttcgtccttcagttgactttccctcttagtttgtaggcgtattcatgaattgttgttccatatgtgtataaatgcacaggaagtgcgtatataccatccacttcaagtaatctaactgagcttaaaatttacagcatcttcggacacaatcctcccgaaagaattatgctgatgcaagccacgtttctgctgtcattttgggtggaggcactggagttcaactctttcctctgacaagcacaagggctacccccgctgtaagaaagtgaaacactatttatatctaatgttgactactgtactgtgcagataatagatttttaattgtggataacatacttagaacttaagaaaaagtttggaatctcttgtcagtttaaactttaaacaagtgctaattttaacttgctgttcttaatgtctgtcgcttaacttgattgagtgactgcaaatcaagttacaacaacatctggtatcatttggttatttcgacttgcttactatgtcatacggttactacattactatccatttggaactactctttctaggggcctagactaatgagacacactaatcaatatcagagtatcacatcaacacaactaagtattagatacaaggatgctaatcaacaagttatctacttttagaacttcatccgagaaaacatatgctagtagtatgttgtattttaccatgatgcctaaaagcatcaaatttaattctctcataaccttaagccatctaactttcactcatccaatcaggctatcaatgactaggagatagatgattcgaaatttcgttgtcaactgtgaatgaataatctaaattctatttttatttttctattacttaggttcctgttggaggatgttacaggcttattgacatccctatgagcaattgcttcaatagcggaataaataaaatatttgtgatgactcagttcaattctgcttctcttaatcgccatatccatcatacataccttggtggggggatcaactttactgatgggtctgtgcaggtaatttacctccatattcttgatatgccttagtggaaaccagttactagcagtagcaggtagcaacattaattgaactaacgttggcaatatataggtattggctgctacacaaatgcctgacgaaccggctggatggttccagggtacagcagatgctatcagaaaatttatgtggatacttgaggtaattaatttgtttcttccctgatattaatgggtaataattcttgcagaatgtaacttttatcatggatcataaaataccccttctattgcattcctgtccaggatcattacaatcaaaacaacattgagcacgttgtaatcttgtgtggagatcagctttacaggatgaattacatggagcttgtgcaggtgcattatgttgtcattctttttacttcatgtattcttgattttcgattgagcaatactttgacatgtaatacttgcagaaacatgtcgatgacaatgctgacattaccatatcatgtgctcccatcgacgggaggtaaattagttcttgtgcatcctgattctatgagcgtgccttatccaacataagatgcatgtgttctaattttcttcttttttattgggaagccgagcttctgactatggactggtgaagtttgatgattcaggccgtgtgatccaatttcttgagaaaccagagggtgctgatttggaatcgatggttagtaattgtcatctttactgttctcttgagaaatcttttctttagttcatctgcacggttggtctgtcagaaataaatatctatttctgcataatgctgcactaaatcaactgatcactgcagaaagttgataccagcttcctcagctatgcaatagatgacaaacaaaagtatccctacattgcatcaatgggaatttatgtcttgaaaaaggatgttcttttagacattctgaagtaagtgtcacccatttgtgatagttcggtccacttcctatgtcttctgataattcgtattcttaggtcaaaatatgcacacctgcaagactttggatcagaaattctcccgagagctgttttagagcataatgtgaaggtaagttccgaacaactgtaatccgggtttcctacataatctccatctattgcttttgcatattcaatggtttagttaatgctacctccacccacagtggacatcaacacagtctgaaaacacaagtttgaccagtagtttccattgtgatatacttatagagtatgataaatttacagtctgaatatccttagtgttttgtggttgctggagcgctgggagtaactagcatgacaacacccagagcaatatacagatcaatgagctttttacattttgcctactctatataacttgaactagcactatattgttttaggcatgtgtcttcacagaatattgggaggatattggaacaatcaaatcattttttgatgctaacttggccctcaccgagcaggtattgccacatatgtattctgtacaactgatgcactctttaacatattgcgagacaccttgtacagtaatacccaaagatgtcttacagagtctaatgatttacccattattcttcagttaacttactgctaacaccaatttgtatacaaaagttgtctgaacatgtttctccgttccagcctccaaagttcgaattttatgatccaaaaactcctttcttcacctcacctcgatacttacctccagcaagattggagaagtgcaaggtatgagtcatgtaaaccacagttgttgcttcagatcacagcaaatgcaatttaaattttggatcttttttttgtgcagatcaaagatgcaataatctccgatggctgttcctttagtgaatgcaccattgagcattctgtcattggaatctcttcacgcgttagcatcgggtgtgaactcaaggtactttatcaagacataaagctcatagtttggacattcaatgcatcaaaatttgatgtcttattaagattagattgattcataggtactgtttggaaactaccaattattctcatgtagttttgcaattctaaaattcacaggataccatgatgatgggtgcagatcaatatgaaactgaagaagaaacttcaaagctattgtttgaaggcaaggtcccaattggtataggcgagaacacaaagataaggtgagccacatctggactaatgcttacatattactgtccttacctggcaatatcgtgtttccaaatgtatgagaccttcgtttcatgaattttaggaactgtatcatcgacatgaatgctagaattggcaggaacgtgatcattgcaaacactcaggtgagcaagtatatgtatttaactactccaaagtcgatgaactcatcttttataaacacttggtaaccacacgagttcactgcgtatgatagggggtccaagagtctgatcaccctgaagagggatactacataaggtctggaattgtggtgatcctgaagaatgcaaccatcaaggacgggaccgtcatataaaagcatagataggccttggaatcgcacctccagtcatctgaaaactggcatccattgatgtgggattttgatgcatgagttggattcaagacggatagcgtagcctccatggcgaagtggtgtttttggtttagctatatgcctatatgcaataagctgcctgaggaagtcgcgcaaccttttgtgtaatttcaggtttatgtacttggtttgttttggaatgggaactctattattggtattctgaagtttgcacctc</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050189.1 RefSeq:Os01g0633100]|
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| − | }}
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| − | [[Category:Genes]] | |
| − | [[Category:Japonica mRNA]]
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| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]] | |
| − | [[Category:Japonica Chromosome 1]]
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| − | [[Category:Chromosome 1]]
| |
