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| − | {{JaponicaGene|
| + | Please input one-sentence summary here. |
| − | GeneName = Os12g0277500|
| |
| − | Description = Similar to RuBisCO subunit binding-protein alpha subunit, chloroplast precursor (60 kDa chaperonin alpha subunit) (CPN-60 alpha) (Fragment)|
| |
| − | Version = NM_001073099.1 GI:115488159 GeneID:4351976|
| |
| − | Length = 4300 bp|
| |
| − | Definition = Oryza sativa Japonica Group Os12g0277500, complete gene.|
| |
| − | Source = Oryza sativa Japonica Group
| |
| | | | |
| − | ORGANISM Oryza sativa Japonica Group
| + | ==Annotated Information== |
| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
| + | |
| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
| + | ===Function=== |
| − | clade; Ehrhartoideae; Oryzeae; Oryza.
| + | * '''''OsCpn60α1''''' encodes Plastid Chaperonin 60α Subunit. |
| − | |
| + | * '''''OsCpn60α1''''' is essential for folding of rbcL. |
| − | Chromosome = [[:category:Japonica Chromosome 12|Chromosome 12]]|
| + | * '''''OsCpn60α1''''' is required for the folding of rbcL and that failure of that process is seedling-lethal. |
| − | AP = Chromosome 12:10292665..10296964|
| + | |
| − | CDS = 10292751..10292807,10293045..10293198,10294104..10294225,10294330..10294537,10294686..10294831<br>,10294962..10295268,10295708..10296159,10296430..10296720|
| + | ===Phenotypic analysis=== |
| − | GCID = <gbrowseImage1>
| + | * Homozygous mutant seedlings were pale green (Figs. 3C and 3D). In progeny of the OsCpn60α1/oscpn60α1 heterozygote, that phenotype was segregated in an approximately 3:1 ratio (WT:oscpn60α1); all pale-green seedlings were oscpn60α1 homozygous plants. This indicated that the mutant phenotype was recessive and was caused by a defect in a single genomic locus. |
| − | name=NC_008405:10292665..10296964
| + | * When leaf chlorophyll contents were measured, the researchers found that both chlorophyll a and b were severely decreased in the mutant, to approximately 37% of that recorded from the WT siblings (Fig. 3E). |
| − | source=RiceChromosome12
| + | * That is, after the fourth leaf emerged from an oscpn60α1 plant, no further leaves were produced, resulting in a lethal character. TEM analyses showed that chloroplast development was impaired in the mutant (Fig.3F). |
| − | preset=GeneLocation
| + | |
| − | </gbrowseImage1>|
| + | |
| − | GSID = <gbrowseImage2>
| + | [[File:146-Os12g0277500.png|center|thumb|727px|'''Figure 3.''' ''Phenotypes of oscpn60α1 mutant.<ref name="ref1" />.'']] |
| − | name=NC_008405:10292665..10296964
| + | |
| − | source=RiceChromosome12
| + | ===Subcellular localization=== |
| − | preset=GeneLocation
| + | * Subcellular localization analysis showed that transiently expressed '''''OsCpn60α1''''':GFP fusion protein was localized to the chloroplast stroma. |
| − | </gbrowseImage2>| | + | |
| − | CDNA = <cdnaseq>atggcgtccgccaacgccatctccaccgcctccctcctccgctctttctcctcccaggggagggtgaggagggccaagaatgggcgggcgcagcggctagtggtgcgggcggacgccaaggacatcgccttcgaccagaaatcccgggcggcgctccaggccggcgtcgagaagctcgccaacgccgttggcgtcaccctcggcccacgagggagaaacgtagtgcttgatgagtacggcagtcccaaagtggtaaatgatggagtcaccattgcccgggctattgagctatatgatccgatggaaaatgctggtgcagcattgatccgtgaggttgctagcaagaccaatgattctgctggtgatgggactacaaccgcctctgtccttgctcgtgaaatcatcaagctgggccttttgagtgtaacttctggtgcaaaccctgtatcacttaagaaaggaatagacaaaacagtgcatggtcttattgaagagctggagaagaaggctagaccagtcaagggcagtggtgatatcaaagctgttgcctctatctctgctggcaacgatgaacttattggatctatgattgctgatgctattgacaaagtgggtcctgatggtgtcctttcaattgagtcttcatcatctttcgagactactgttgatgttgaagaaggaatggagattgaccgtggatacatttcccctcaatttgtgacaaacctcgagaaatcgatcgtagagtttgagaatgctaaggttcttattactgatcagaagatcaccagcataaaggagattcttccaatcttggagaagacaacacagttgagagctccactgtttattattgctgaggacattactggcgaagctttggcaacccttgttgttaataagctccgaggaattctcaatgttgcagcaatcaaagccccaagttttggtgagcgacgcaaggctgtccttcaggatattgccattgtgacaggcgctgaatttctagcaaaagacctgggtttgctggttgagaatgcaactgaagagcaacttgggacagcgagaaaagtcacaatacatcagactacaacaaccctcatagcggatgcagctagcaaagatgagattcaggcaagggttgcacagctaaagaaggaactctccgaaactgattcgatatatgatacagagaaattggctgagaggattgcaaagctttctggtggtgtggctgtcatcaaggttggggctgcaactgagactgagcttgaggaccgccaactacgtatcgaggatgcgaagaatgcaactttcgcagctattgaggagggcattgttcctggaggtggtacagcgtatgtgcatctttcaactaccgtccctgcgatcaaagaaacaatcgaagaccatgatgagcgccttggtgcagacattattcaaaaggcgttggtagctcccgcttcactgattgcccacaatgctggagtggaaggggaagtggtagttgagaagatcaaggacggcgagtgggaagtcgggtacaatgcgatgaacgacaagtatgagaacttgattgaggcgggtgttattgacccagctaaggtgacgagatgcgcgctccagaatgccgcctcggtggctggaatggtccttaccacacaggccattgttgtcgagaagccgaagcccaaggctcctgttgctgagccggcagagggaacccttaccgtctaa</cdnaseq>|
| + | You can also add sub-section(s) at will. |
| − | AA = <aaseq>MASANAISTASLLRSFSSQGRVRRAKNGRAQRLVVRADAKDIAF DQKSRAALQAGVEKLANAVGVTLGPRGRNVVLDEYGSPKVVNDGVTIARAIELYDPME NAGAALIREVASKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVH GLIEELEKKARPVKGSGDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSF ETTVDVEEGMEIDRGYISPQFVTNLEKSIVEFENAKVLITDQKITSIKEILPILEKTT QLRAPLFIIAEDITGEALATLVVNKLRGILNVAAIKAPSFGERRKAVLQDIAIVTGAE FLAKDLGLLVENATEEQLGTARKVTIHQTTTTLIADAASKDEIQARVAQLKKELSETD SIYDTEKLAERIAKLSGGVAVIKVGAATETELEDRQLRIEDAKNATFAAIEEGIVPGG GTAYVHLSTTVPAIKETIEDHDERLGADIIQKALVAPASLIAHNAGVEGEVVVEKIKD GEWEVGYNAMNDKYENLIEAGVIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKA PVAEPAEGTLTV</aaseq>|
| + | |
| − | DNA = <dnaseqindica>87..143#381..534#1440..1561#1666..1873#2022..2167#2298..2604#3044..3495#3766..4056#actcttccgcccccaaatcctcctgctcacctcaccttatcctcagctctcagcacctcttcctccctcccccaagcttccctctaatggcgtccgccaacgccatctccaccgcctccctcctccgctctttctcctcccaggtcagcccccccgcctctgtccttcgttgtttcttccacaattacggccttctctctcttttttatttttgtgggtttctgagctgttgcttcttgggtgaattgggggaagctgttgcttcttgaattctgctagccaggacaatttgagcttggttttctcctccagatttcgggtatactactcagttctgcacaatttagtgagctgaattttgtttgttttgccgcaagcaggggagggtgaggagggccaagaatgggcgggcgcagcggctagtggtgcgggcggacgccaaggacatcgccttcgaccagaaatcccgggcggcgctccaggccggcgtcgagaagctcgccaacgccgttggcgtcaccctcggcccacgaggtgatgcatcttgctcacttcacttcacttcccttatcttctttcccttttttttggggagagaaagttccttggctagaattagcaccctttaatccattgtggctacgcctattgcgagtaatgtttttgtagataatggaattcatcgacttgaaagattacagcggttgcaggacgaatgattgcgaattacagaattacggcttaattattgtgacttgtgttaatcagataatgtcagctaatggctgaaagtgctaatttgtgctacccattcataactgcaagttgcattgcgttatttggtgccataacaacataaggctatgaaaaagctttgtatattcagcagtatcttcaggcttcatatatagctgatgaagtgtcgtttagttcgctgatttcatcagcagcggcacctgcgggcagttccgtgatacagttttagccttttagcagtcttgttatgagttgtttaggtgagacataaaaccgcataaagttgtaaactttattgaatctgatttggcatttttcatcatttagtgatgacggccaaggtgcagatatggcttgactttagtcacacagtacaatatatgtagttgacttacatagctgtgtccatggtgatatttgatcattggttgattattgttgtcatatttagaggtatgcgcattgtgcacccatgatggagaggcccaatgtatctcacactcctcctttatctagtaaaatgatttgggtgtcacctcgtctggtttctctactctctatactttgcatacggagtacttcttttgtcagtttagttttttaatactagcatgttccatcaataaaacaactgtagtgttttctttcatgatgcaagctctgatcttagggatgttttacagggagaaacgtagtgcttgatgagtacggcagtcccaaagtggtaaatgatggagtcaccattgcccgggctattgagctatatgatccgatggaaaatgctggtgcagcattgatccgtgaggtgactttgtttttgttcagatgcattttttatcattatattttatttcagaagatgggcttcctaaagcatgttgtccacctttttcatggttgcacttgaaggttgctagcaagaccaatgattctgctggtgatgggactacaaccgcctctgtccttgctcgtgaaatcatcaagctgggccttttgagtgtaacttctggtgcaaaccctgtatcacttaagaaaggaatagacaaaacagtgcatggtcttattgaagagctggagaagaaggctagaccagtcaagggcagtggtgatatcaaaggtgatgctgttgtatcatgttctgccatgtattttgttctgattttcacatcttagtgtttactagataacaaggctggccgtattttaaacaagtgaaaattcggaggatgataggtttctaatttggagcttgttttgtgttacagctgttgcctctatctctgctggcaacgatgaacttattggatctatgattgctgatgctattgacaaagtgggtcctgatggtgtcctttcaattgagtcttcatcatctttcgagactactgttgatgttgaagaaggaatggaggtattttgaaattccaatatacacttttgttgcccttttatccagttgcaacttcagattcacgtgaagtattccaataaataaatagtgtgacaacaacttcctcaagcattcttaaattttggtgcagattgaccgtggatacatttcccctcaatttgtgacaaacctcgagaaatcgatcgtagagtttgagaatgctaaggttcttattactgatcagaagatcaccagcataaaggagattcttccaatcttggagaagacaacacagttgagagctccactgtttattattgctgaggacattactggcgaagctttggcaacccttgttgttaataagctccgaggaattctcaatgttgcagcaatcaaagccccaagttttggtgagcgacgcaaggctgtccttcaggatattgccattgtgacaggtttgtctcacatccaacaatgacattgtttttgttaaatttaacttcaataatgtataaaatgtagtcgtcgtgtgctgaggccacatttcctttgaatcatctaagccatgttagttacttgatatcttttgagagttaccaaaaccctaacaatgttttggtctcaaattatcagcttgcaagcaaaattgtggttataggtgcaaaagcatcttacattgtaatctacctttatgaatttgataagagctcacagcaagcaatggcaatcccttatctgaccgtcctgtaattgcctacttactcaaaacttgaatcttccaacacgttgcttgtttatatttctagttcatggggaatatacattttatactgcatataatacacttgcaccttttctgctgctgcttttttcttattctatatgtttctccaggcgctgaatttctagcaaaagacctgggtttgctggttgagaatgcaactgaagagcaacttgggacagcgagaaaagtcacaatacatcagactacaacaaccctcatagcggatgcagctagcaaagatgagattcaggcaagggttgcacagctaaagaaggaactctccgaaactgattcgatatatgatacagagaaattggctgagaggattgcaaagctttctggtggtgtggctgtcatcaaggttggggctgcaactgagactgagcttgaggaccgccaactacgtatcgaggatgcgaagaatgcaactttcgcagctattgaggagggcattgttcctggaggtggtacagcgtatgtgcatctttcaactaccgtccctgcgatcaaagaaacaatcgaagaccatgatgagcgccttggtgcagacattattcaaaaggtatcttgtctattgatttgtctgcattggctgttgcttatagaactaagaggaaatggaatgcaatttacaagagaagcatctcatgaacgaatctttgcattatgtttgtctgtggtcaaataaagctaggacttgcactatatagtcgatgaataggtgaatagtgaccgaataattttggatgcatatgcattgcatatagtactagttctacacttcagttaaatataagaacatagttgacagcatatcttgtaaattttgcaggcgttggtagctcccgcttcactgattgcccacaatgctggagtggaaggggaagtggtagttgagaagatcaaggacggcgagtgggaagtcgggtacaatgcgatgaacgacaagtatgagaacttgattgaggcgggtgttattgacccagctaaggtgacgagatgcgcgctccagaatgccgcctcggtggctggaatggtccttaccacacaggccattgttgtcgagaagccgaagcccaaggctcctgttgctgagccggcagagggaacccttaccgtctaaacagtggtgaatttcttcagggtggctttgtttagatggttcataccaactgaaatggtagaatttttgtctgggagtttttggttcagatcaaccgaaaggtctagtgtaggattggtttccatttttgagattgaaggtgcaacctgcctgatctgaactcatatgtccatcaaagaaataatttgcatcattatcgtcatcttcggtttaaactttataaagtaaatcatatggtgtttct</dnaseqindica>|
| + | ==Labs working on this gene== |
| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001073099.1 RefSeq:Os12g0277500]|
| + | * Crop Biotech Institute and Department of Genetic Engineering, Kyung Hee University, Yongin 446-701, Korea |
| − | }}
| + | |
| − | [[Category:Genes]] | + | ==References== |
| − | [[Category:Japonica mRNA]]
| + | <references> |
| − | [[Category:Oryza Sativa Japonica Group]] | + | * <ref name="ref1"> |
| − | [[Category:Japonica Genes]]
| + | Kim SR, Yang JI, An G. OsCpn60α1, encoding the plastid chaperonin 60α subunit, |
| − | [[Category:Japonica Chromosome 12]] | + | is essential for folding of rbcL. Mol Cells. 2013 May;35(5):402-9. doi: |
| − | [[Category:Chromosome 12]]
| + | 10.1007/s10059-013-2337-2. Epub 2013 Apr 24. PubMed PMID: 23620301; PubMed |
| | + | Central PMCID: PMC3887859. |
| | + | </ref> |
| | + | </references> |
| | + | |
| | + | ==Structured Information== |
| | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 12]] |
