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| − | {{JaponicaGene|
| + | The rice '''''Os07g0203700''''' was reported as '''''SPL5''''' in 2012 <ref name="ref1" /> by researchers from China. |
| − | GeneName = Os07g0203700|
| |
| − | Description = Cytochrome cd1-nitrite reductase-like, C-terminal haem d1 domain containing protein|
| |
| − | Version = NM_001065675.1 GI:115471082 GeneID:4342674|
| |
| − | Length = 7981 bp|
| |
| − | Definition = Oryza sativa Japonica Group Os07g0203700, complete gene.|
| |
| − | Source = Oryza sativa Japonica Group
| |
| | | | |
| − | ORGANISM Oryza sativa Japonica Group
| + | ==Annotated Information== |
| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
| + | ===Gene Symbol=== |
| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
| + | *'''''Os07g0203700''''' '''<=>''' '''''SPL5,OsSPL5''''' |
| − | clade; Ehrhartoideae; Oryzeae; Oryza.
| + | ===Function=== |
| − | |
| + | * '''''SPL5''''' encodes a putative splicing factor 3b subunit 3 (SF3b3) |
| − | Chromosome = [[:category:Japonica Chromosome 7|Chromosome 7]]|
| + | * '''''SPL5''''' is a cell death and defense-related gene in rice |
| − | AP = Chromosome 7:5599866..5607846|
| + | * '''''SPL5''''' might be involved in splicing reactions of pre-mature RNAs participating in the regulation of cell death and resistance responses. |
| − | CDS = 5600201..5600374,5600455..5600563,5601075..5601196,5602859..5602935,5603349..5603487<br>,5603586..5603741,5604306..5604618,5605084..5605394,5607245..5607844<br>|
| + | * '''''SPL5''''' negatively regulates cell death and resistance responses via modulating RNA splicing in plants. |
| − | GCID = <gbrowseImage1>
| + | ===Phenotypic analysis=== |
| − | name=NC_008400:5599866..5607846
| + | * Rice lesion mimic mutant spotted leaf 5 (spl5), induced by a radiation, spontaneously occurs cell death mainly in leaves. Under natural field conditions (25–35°C), the spl5 mutant displayed relatively small reddish brown necrotic lesions on leaves as early as 15–20 days after sowing, and then lesions continuously developed and finally scattered over the whole leaf surface (Fig. 1b, d). |
| − | source=RiceChromosome07
| + | * Even under aseptic conditions, these lesions were still formed on the mutant leaves (Fig. 1e, f). Leaves from the mutant and wild-type Zhefu 802 were respectively stained with trypan blue, a histochemical indicator of irreversible membrane damage or cell death (Dietrich et al. 1994). |
| − | preset=GeneLocation
| + | * The trypan-blue staining revealed that numerous microscopic lesions consists of clusters of a few dead cells throughout the spl5 leaves, and on the contrary, no necrotic lesion was observed on the wild- type leaves (Fig. 1g, h). |
| − | </gbrowseImage1>|
| + | * Further histochemical assays showed that HR-marker compounds, reactive oxygen species (ROS) such as H 2 O 2 and callose were greatly accumulated in the mutant leaves, whereas their accumulation were not observed in the wild-type leaves (Fig. 1i–l). |
| − | GSID = <gbrowseImage2>
| + | |
| − | name=NC_008400:5599866..5607846
| + | |
| − | source=RiceChromosome07
| + | [[File:173-Os07g0203700.png|center|thumb|727px|'''Figure 1.''' ''HR-like lesion mimic phenotypes of the spl5 mutant.<ref name="ref1" />.'']] |
| − | preset=GeneLocation
| + | You can also add sub-section(s) at will. |
| − | </gbrowseImage2>| | + | |
| − | CDNA = <cdnaseq>agacttgataattcatcttctagttgtgcaacgagtggtgattttcggaaagattttgcagctaatattcgcaagtttgctgtaattggaactcacgaaccttctgtgcatatcatttcgttggaacctggagaagcatttcagcaacttgctgttgggcatatatcagtaaacaacgcgctcggcactcctatcagtggttgtatacctgaaaatgttaggtttgtagcagctgcaaggttctacattcttgcaggactaaggaatggaatgctactgaggtttgagtcacaaacaagcaaagggcattgtttccctggttccttttacaaggagtcctctaccccttgtgatgatacatcccttatgttgattgctgtacggcgtattggaatcactcctgtagttttggtaccgttgcatgacagagccaatgctgacattattgttctcggtgataggccttggctattgcaatctgctagacacagcttggcatattcatccatctcatttctgcctgcttcccatgtgacaccagtgtcttctactgattgccccagtggtctgctgtttgtttcggagaactgtttgcatttggttgaaatggttcatggcaaaagactaaatgcacagaagttttcgattggagggactccaaggaaagtgttataccacagtgatagtagaacactgttggtgctgagaactgggttaaccagtgtatcctgttcttcagatattgttcaaatagatccaagtaatggggcattgctttccagattcaagtgcgaacctggtgaaacagcaaaatgcatgcaaattgcaaagatagggaatgatcaagtattaatcgttggaactagtaagtccaatggccgaccaatgatgccaaacggtgaagcagaaagtatcaagggtcgtctaattcttttaagcttggagactattgaaagtcctcgtgagagcggttcgtttactgcagcttctaatttgaattcatctcatgctggttctccattcccggaatttgttggatatgcagcagaggagctgtctagtaacagcatgtgcagcagtcctgatgaagtttgctgcaatcaaattcaacctgaactcatggcaggccatttgagatcactagtccaacatacattcaatggcgctgttcttgctgtacatccatatctagatagatatgtgttggcagcagctggaaatgtgctttttgtatttggatttttaaatgaaagtccccatcggataaaaaagtatactacgagtaggacacgatttacaataacctgtttgaagacgtatgcatcacggattgcagtgggtgattgccgcgatggtgttctcttctattcttatcatgagaatcttaggaagttggaactgatctattctgatcctgcccaacggctagtgggtgatgttgcccttttgagctgtgaaactgctgttgtatcagatcgacgagggagcatatctgtactgtcttgcccaagattagaagtatccgaaagtccagagaagaacctagcggtgcattgttcattttacatgggtgaaacagctatgagcatccagaaggttgcatttaagcattggcttccaattgatgatttgacagaaccagtgttggagtcggtatacaactgtgtcgtcgcaagcactctgctggggagtatctttgttatgattccactcacaagtgaggagcatcaaatgttgcaagatgtccaagaaagactttcggttcacccattgactgctccactcttaggaaatgatcatgcagagtttcgtcgccgtggtatcccgtcgggagtaccgcccattctggatggcgacatgcttgtgcagttcctggagctcaccagcgaacaacagcacgatgttctcaatattgtttctcctggaaagaagaggcaacacgatatctcggttttccaggtcatgcgagcattagagagggtgcattacgcactcaactga</cdnaseq>|
| + | ==Labs working on this gene== |
| − | AA = <aaseq>RLDNSSSSCATSGDFRKDFAANIRKFAVIGTHEPSVHIISLEPG EAFQQLAVGHISVNNALGTPISGCIPENVRFVAAARFYILAGLRNGMLLRFESQTSKG HCFPGSFYKESSTPCDDTSLMLIAVRRIGITPVVLVPLHDRANADIIVLGDRPWLLQS ARHSLAYSSISFLPASHVTPVSSTDCPSGLLFVSENCLHLVEMVHGKRLNAQKFSIGG TPRKVLYHSDSRTLLVLRTGLTSVSCSSDIVQIDPSNGALLSRFKCEPGETAKCMQIA KIGNDQVLIVGTSKSNGRPMMPNGEAESIKGRLILLSLETIESPRESGSFTAASNLNS SHAGSPFPEFVGYAAEELSSNSMCSSPDEVCCNQIQPELMAGHLRSLVQHTFNGAVLA VHPYLDRYVLAAAGNVLFVFGFLNESPHRIKKYTTSRTRFTITCLKTYASRIAVGDCR DGVLFYSYHENLRKLELIYSDPAQRLVGDVALLSCETAVVSDRRGSISVLSCPRLEVS ESPEKNLAVHCSFYMGETAMSIQKVAFKHWLPIDDLTEPVLESVYNCVVASTLLGSIF VMIPLTSEEHQMLQDVQERLSVHPLTAPLLGNDHAEFRRRGIPSGVPPILDGDMLVQF LELTSEQQHDVLNIVSPGKKRQHDISVFQVMRALERVHYALN</aaseq>|
| + | * College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China |
| − | DNA = <dnaseqindica>7473..7646#7284..7392#6651..6772#4912..4988#4360..4498#4106..4261#3229..3541#2453..2763#3..602#ggagacttgataattcatcttctagttgtgcaacgagtggtgattttcggaaagattttgcagctaatattcgcaagtttgctgtaattggaactcacgaaccttctgtgcatatcatttcgttggaacctggagaagcatttcagcaacttgctgttgggcatatatcagtaaacaacgcgctcggcactcctatcagtggttgtatacctgaaaatgttaggtttgtagcagctgcaaggttctacattcttgcaggactaaggaatggaatgctactgaggtttgagtcacaaacaagcaaagggcattgtttccctggttccttttacaaggagtcctctaccccttgtgatgatacatcccttatgttgattgctgtacggcgtattggaatcactcctgtagttttggtaccgttgcatgacagagccaatgctgacattattgttctcggtgataggccttggctattgcaatctgctagacacagcttggcatattcatccatctcatttctgcctgcttcccatgtgacaccagtgtcttctactgattgccccagtggtctgctgtttgtttcggagaactgtttgcatttggtaagtttctaggactcgtgaaagtgaaatccacaagcaaaacatgcacatattcttttttagggatcaagaataattacattattgcttgcatttcatgctctgaattattaggactcataaaccatgagattgctaaaatctgggatcgaatcccattataatgagttctccaaatagatagtcaggaacatagaaatcatggctgttgattgttatcttataattttctttatgcactcggtgaaaccgtattctctcttctggcatcaaaatttcacacttctcttttttgctgctatttctttagtatccgtttctcttttgccactatcgttggtcaacatccgttgaccatcacatttgccaataaaaaactgttgtacccttgcgaaactaacaaaatataagaaattcaaactatctaggaaaatacaatttacctttttagtttttaaaggttgtacaaataattatggtaggaaaattttcagttagtttaaaaggttcccacgtgtatcttaaaaatggaatttttaaaggaaatatttttgcatatccacatacaaatcaaacagcatagctaccaatattcatgtaaacaaccatgcacataattccttagtatagattattctgtgaaacatttgagaaccaaatcattcccatgctacatttttaaagattaatattagtgataatataattcccaattttatgtttttctaaaaaagtgtattaagtggccataatttagttttcttgataactttatggtataggttaaaatgtcaagtgctgcattattttggaacatgtacaggggtataatggccttttaggggctaacttaacagtcaatggatgttgactagacggtaatgatgaaacaaaaatttgctctgacagaatagtggcaaaagagagaagtgcagattttcgatggcggaagagagaacacagttgcaatgagtgacaaaaagaaaaaaaatcccttgttttattatacatagaactaatttgattcgtgacatcgaaatggtactcacagattttccatccatggtttctctcatatcattgtatttgagattttctttttgctagcatataatttttgaaaactactccctctgtcccataataagttcatttttggtttcttctattttccccaaaacaagttcatttttgaagaatcattgcagagtttgtggaagtaaggaatgattatttgggagaagataaagtgaggaatagttgcattgcgatatgataaagtagggatattctagttgtatatgtgtgggatgggtgaaaaatgaacttatttagggacggaaggagtaactaggtaatgtcatgtaccttgtagttttctctaatggggcattttaccagaggggtggcaatgtagcagagagtgtgtcaccatgtcaagtaaaaaaccctcccattctgtattctattttccccatggccaaagggactttagtccttttcttgttcgtctgaaacatgaaatctcttaatcgtagtcatcctgttttagcctttccctgttaagtttatgtgcttatatttcaagtagatttcttttgttccagtcccttctgttgatctgccataagtatggtgcaattactaggataatttattctaaaataccaagatgataataaaatcatgaccttgtgggtattgttgaaacttgaaagcaatgcttatattattctctcactattggtatgcatgtatgtgttctcacatagtcacatgtttatctcctaaaagtggcttgcatgtgcatttcattgtactgtcagtttaaatcaagtttatcttttcaggttgaaatggttcatggcaaaagactaaatgcacagaagttttcgattggagggactccaaggaaagtgttataccacagtgatagtagaacactgttggtgctgagaactgggttaaccagtgtatcctgttcttcagatattgttcaaatagatccaagtaatggggcattgctttccagattcaagtgcgaacctggtgaaacagcaaaatgcatgcaaattgcaaagatagggaatgatcaagtattaatcgttggaactagtaagtccaatggccgaccaatgatgccaaacggtgaagcagaaaggtaaggacgacaactcatgttttgtttctgctgaaccttgttttgtcaagtttgaagtgtccatggtcccttagaatttttgcactactaatgtttaacttgcaatcctagtgttttttttttgcttggtttgagtataattattaatattaatgatctgttgttttggtctccacttttttgttttctgtctgcccgttgtctggtattctgcaaaagtgacatgggtatgaatttgcaaagcttgcaaccatgaggcacattttttggtttctcactgattgtgacggtgttgccattgcgctattgatcattcaatgcatatttgcagttggatcatcaatatttttttcttgttttttctgcacttaatttagtttttccatttccatatctttgaattgtgtgtatagttaaaagctgcctttatattgttattataactattctctttggatgaaacagtatcaagggtcgtctaattcttttaagcttggagactattgaaagtcctcgtgagagcggttcgtttactgcagcttctaatttgaattcatctcatgctggttctccattcccggaatttgttggatatgcagcagaggagctgtctagtaacagcatgtgcagcagtcctgatgaagtttgctgcaatcaaattcaacctgaactcatggcaggccatttgagatcactagtccaacatacattcaatggcgctgttcttgctgtacatccatatctagatagatatgtgttggcagcagctggaaatgtggtatgattagaagtcaatttttatcatatatgatagtatatctattaaagagtagcttagctcggtaatggatctagataaataaatttgcaaagaagcttgaatgcttgattggcaagttccggactcttgacatcagaatcagttaatcaacatttatcctggcaaataacactatttatgtggatcgtatggtgttggcttctttcacttctatagattgctagaacaacagaacgaaggtgcattagcactttagcatatgagccacattaatagcagaggatttttttttgtaataactactggagagatagactagtgacgattggagttgaggagatggagattggaagggttatttgccattataaaaaaaatcctgaaagaatatcccaacaagagtgttattattttccaaaacaaacagttatcatgacgtcactaaattttctatcattctgttactatctggtgctaatatgttgatgctgacagatccagtttgaataccacaatatttgaaaatcatgatctgagattttctctgccttatcttgacagctttttgtatttggatttttaaatgaaagtccccatcggataaaaaagtatactacgagtaggacacgatttacaataacctgtttgaagacgtatgcatcacggattgcagtgggtgattgccgcgatggtgttctcttctattcttatcatgaggtacaagttgattcaattgaaacaatacatttttatgatgtttatttttatggagcttctttgtcgttacctctaatcctcgtattgatttatgccagaatcttaggaagttggaactgatctattctgatcctgcccaacggctagtgggtgatgttgcccttttgagctgtgaaactgctgttgtatcagatcgacgagggagcatatctgtactgtcttgcccaagattagaaggtatgccttggtatagatgtctatgttatccctttctttttagtttgtacctgttcttttctctggattctttttaatggtagtgcctcctctatactcttctgcatcttggtcatactttgtaattttgtagccttcatgtttagtttctcaatctaattcctttattgacatactaaatatgcagtataaacatcacaatatagctggcataaaatttgccagtgtagtaaggccacatagttgaatattgtgcctagaagctttatgaatttcgtaatagaattcaaaattttgcattacgtttgtgtacttctcatcatatatgcattactttgtaatatctacctaaattaatttaaattttacattacgtcaagatttattttgactttttttaacatatcttgcagtatccgaaagtccagagaagaacctagcggtgcattgttcattttacatgggtgaaacagctatgagcatccagaaggtaagcttctgttggcatcttagggctaaccagtgtgaattccattaacgaaaacataccaaagagagggttttgaacatgggtattgggtagctgctttggatttaaaggatgaatcatgtgtttgtaacttgatgaactaatgggctgtttggtttgaggaatcaaatcatcctaggatgagttttttccagcatgacccatcatgagtaggggtgaaacagtgcggaaacggacggaaacaacctttatcgttttcgttttcatattttttttggaatcggaaaccccgaaaacgaatattttcgaaactgaaaacagagcgaaaacgaaccggtgcggatacggtaatgaaaatttatcggaatacaaaaacccctcaaactgagcttcaaacttttgtcaagtctaattctcaagtctcaacagtcaacaattcgtcataaaacacaattatatcaagtctaattgttaagtctcaacacaagtacacaataacacttaacacggagtgacggaggtggaggttgaggaataacacctgctggctgctgccactgggctgctggctgctgcgaggcgaggcgttgtcggcggtgaggaagagtcgccagtggggcttgaggtgtcgtagacttgtcggtgtggctatggcggcgtcgtcagtggtggtggcggcggcgaaacggcgagacggcggcgcgaaacggcgggacggcgccgcgagatggtggcggtacgagacggcggtatgggacaacgaagagcgactgcgagagaggaagggtgggcacggtcgcgaggacggcaggggcgaggtcacgaggagccgaggacggcagaccagcggtggcgcgggcggtgggcgctggggagcacgatgccgtgatctggattctggatgcgctgctccgccgccggctcgcggtcgcgcgatgccgtgctcccgtgcagcgatcagtgtgtctctcctctctagggttagccgctatttgggtagatgctgcgatttggattgggctgggaacttggggacgacttatagactattgattctatgggcctttttaacttcagaaattccggcaaattttttggaatttttccgaccgattccgagttccgacggaaactgctattatcatattcgatttcgtttccgagaaaatatttccgttttcgattccgtttctgagaaattctgaaaaaattctgaccagctgattccatttccgaaaacaggcccggaatccggaaagtttccgtaccgttttcacccctaatcttgaggtcatcccatgattttgatgggatgatccaatgatcccattcatctttttttttccaactaagcatgtcgctttgaggaatgaggtagaaatttagtagtccattctgctcttgtttggttcggaagttggacctgctggtgcattgagtatccaattacttagatgaaacagcccataattgatctaggagatttgccttgttatctctcatgatcccgttattttccctgaacatttggttttcattcatcatgacccgacccttttctttctatatgagccccatggagaagtgtgaccgaactagtttcatgttttatattgttgctttgctttcctatggttaattcggtcttgtaatttgctgtaggttgcatttaagcattggcttccaattgatgatttgacagaaccagtgttggagtcggtatacaactgtgtcgtcgcaagcactctgctggggagtatctttgttatgattccactcacaaggtgtagttttgaagattccaactctagaatctaaattcatgctattagctatttatctgtgacagctcagtcagggccactgctagcctgtcggtaacatctaaaccaatttgggagattgaatttgctttaagttaatcagggttgtgaactatgatagttttggctaagcttcacaattatatttggtacatgatttttgaaatgttattcacataaataataaaatagaacactttatcgtttagaattatttgttgctatgctgttcctgaggactagatgccatacacattgataatgtaaacaaagtagtcaatgctcgcaccattactaattaaatccaaattgattgtgtttaacctgataatttgtttgcgtactagtgtcgacaactaattggtaaactaagccctttcccattctatatccttttgtaacaaggggccagagttattttatctctgcacactagtgttatgattctgaagctaatgaattggtaatgcagtgaggagcatcaaatgttgcaagatgtccaagaaagactttcggttcacccattgactgctccactcttaggaaatgatcatgcagagtttcgtcgccgtggtatcccggtaagataattaaactgccatactgaaagctagttttttttgcaagtgttgaataaccttatacactactgctatgacagtcgggagtaccgcccattctggatggcgacatgcttgtgcagttcctggagctcaccagcgaacaacagcacgatgttctcaatattgtttctcctggaaagaagaggcaacacgatatctcggttttccaggtcatgcgagcattagagagggtgcattacgcactcaactgaggcgttcaacaatccattcaacccggtcactgttcttaccgacatggctgtctccaacctgctgatatttgggtggcatcatcgctgaatttggatccgagccgagcaccgagttgttgcagctgaagctgctactggagcacatacccagaacataccaaatctcagctgtagtgaggtgctgtgttttgctgtgggatgataggcccatctgatgtcgcaatagtgtgtcattgctctagtgcaatctttgtaaataacgaataatattttggcagaaggatgtggatacaaatccttttgcccagtgaaaggaacaaccctcgtgaacgcat</dnaseqindica>|
| + | * Institute of Genetic and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China |
| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065675.1 RefSeq:Os07g0203700]|
| + | * China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, China |
| − | }}
| + | |
| − | [[Category:Genes]] | + | ==References== |
| − | [[Category:Japonica mRNA]]
| + | <references> |
| − | [[Category:Oryza Sativa Japonica Group]] | + | * <ref name="ref1"> |
| − | [[Category:Japonica Genes]]
| + | Chen, Xifeng, et al. "SPL5, a cell death and defense-related gene, encodes a putative splicing factor 3b subunit 3 (SF3b3) in rice." Molecular breeding 30.2 (2012): 939-949. |
| − | [[Category:Japonica Chromosome 7]] | + | </ref> |
| − | [[Category:Chromosome 7]]
| + | |
| | + | </references> |
| | + | |
| | + | ==Structured Information== |
| | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 7]] |
