Difference between revisions of "Os03g0752200"

From RiceWiki
Jump to: navigation, search
(Annotated Information)
 
(5 intermediate revisions by 2 users not shown)
Line 1: Line 1:
Please input one-sentence summary here.
+
The rice '''Os03g0752200''' was reported as '''OsSPO11-1''' in 2010 <ref name="ref1" /> by researchers from the China.  
 
+
<br>
 
==Annotated Information==
 
==Annotated Information==
 +
===Gene Symbol===
 +
*'''Os03g0752200 <=>OsSPO11-1'''
 +
<br>
 
===Function===
 
===Function===
Please input function information here.
+
*Spo11 is a homolog of a subunit of archaebacterial topoisomerase, which catalyzes DNA double-strand breaks and initiates homologous chromosome recombination.  
 
+
*OsSPO11-1 is essential for both homologous chromosomes pairing and crossover formation during meiosis in rice.
===Expression===
+
<br>
Please input expression information here.
+
===Mutation===
 
+
*The researchers conducted RT-PCR to confirm that the mutant phenotype of the transgenic plants was caused by the defect of OsSPO11-1 gene. Total RNA was extracted from the prepollinated flowers of both wild type and the completely sterile transgenic plants. RT-PCR analysis showed that OsSPO11-1 transcripts in the transgenic plants were downregulated, suggesting that the sterility was caused by the knockdown of OsSPO11-1 gene in these transgenic plants.
===Evolution===
+
<br>
Please input evolution information here.
 
 
 
You can also add sub-section(s) at will.
 
  
 
==Labs working on this gene==
 
==Labs working on this gene==
Please input related labs here.
+
*Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University.
 
+
*State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,
 +
<br>
 
==References==
 
==References==
Please input cited references here.
+
<references>
 +
<ref name="ref1">
 +
OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice
 +
</ref>
  
 +
</references>
 +
<br>
 
==Structured Information==
 
==Structured Information==
{{JaponicaGene|
+
    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]
GeneName = Os03g0752200|
 
Description = Similar to AtSPO11-1|
 
Version = NM_001186714.1 GI:297722558 GeneID:9267055|
 
Length = 3710 bp|
 
Definition = Oryza sativa Japonica Group Os03g0752200, complete gene.|
 
Source = Oryza sativa Japonica Group
 
 
 
  ORGANISM  Oryza sativa Japonica Group
 
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
 
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
 
            clade; Ehrhartoideae; Oryzeae; Oryza.
 
|
 
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
 
AP = Chromosome 3:31773181..31776890|
 
CDS = 31773181..31773280,31773375..31773486,31773564..31773640,31773727..31773828,31773923..31773996<br>,31774231..31774247,31774537..31774606,31774694..31774730,31775206..31775253<br>,31775346..31775404,31775494..31775523,31775601..31775708,31776100..31776220<br>,31776339..31776402,31776764..31776890|
 
GCID = <gbrowseImage1>
 
name=NC_008396:31773181..31776890
 
source=RiceChromosome03
 
preset=GeneLocation
 
</gbrowseImage1>|
 
GSID = <gbrowseImage2>
 
name=NC_008396:31773181..31776890
 
source=RiceChromosome03
 
preset=GeneLocation
 
</gbrowseImage2>|
 
CDNA = <cdnaseq>atggcggggagggagaagaggcggcgggtggcggcgctcgacggcgaagagcggcggcggcggcaggaggaagcggcgaccctcctccacaggatcagagggttggtgcgctgggtcgtcgccgaggtcgccgccggccgctccccgaccgtcgcgctccaccgctaccagaactactgctcctccgcctccgccgccgccgcgtccccatgcgcctgcagctacgacgtccccgtgggcacagatgtactctcgctcctccaccggggctcccacgcctcccggctcaatgtgctcctcagggttcttctcgtcgtgcagcaactcctgcagcagaacaagcactgctccaagagggacatctactacatgtacccctccatattccaagaacaagcggttgttgaccgtgcaatcaatgatatatgcgtactcttcaagtgcagccggcacaatctcaatgtggttcctgtggcaaaaggcttggtgatgggctggattagatttttagagggagaaaaggaagtctattgtgtaacgaatgtcaatgctgccttctctatcccagttagcatcgaagcaataaaagatgttgttagtgttgcggactacatacttattgtcgagaaggagacagtgtttcaacgtttggctaatgacaagttctgtgaaaggaatcgctgcattgtgattacaggaagaggctacccagatattccaacaagaagattcttgcgctaccttgttgaacagctgcatttgccagtttactgtttggtggatgcagacccttatggtttcgacattctggctacctacaaatttggttcactgcaattggcatacgatgcaaatttcctgcgtgtgcctgatattcggtggcttggggtcttcacatctgattttgaggattatcgccttccagactgctgcctacttcacttgtcgtctgaagacagaaggaaagctgaaggaattctctcaaggtgttacttgcacagggaagccccacaatggaggttggagttagaagccatgttgcaaaagggtgtcaaatttgagattgaggcgttatctgcatgttccatttcctttttatcagaagagtacattcccaagaagatcaaacaaggaagacatatataa</cdnaseq>|
 
AA = <aaseq>MAGREKRRRVAALDGEERRRRQEEAATLLHRIRGLVRWVVAEVA                    AGRSPTVALHRYQNYCSSASAAAASPCACSYDVPVGTDVLSLLHRGSHASRLNVLLRV                    LLVVQQLLQQNKHCSKRDIYYMYPSIFQEQAVVDRAINDICVLFKCSRHNLNVVPVAK                    GLVMGWIRFLEGEKEVYCVTNVNAAFSIPVSIEAIKDVVSVADYILIVEKETVFQRLA                    NDKFCERNRCIVITGRGYPDIPTRRFLRYLVEQLHLPVYCLVDADPYGFDILATYKFG                    SLQLAYDANFLRVPDIRWLGVFTSDFEDYRLPDCCLLHLSSEDRRKAEGILSRCYLHR                    EAPQWRLELEAMLQKGVKFEIEALSACSISFLSEEYIPKKIKQGRHI</aaseq>|
 
DNA = <dnaseqindica>1..100#195..306#384..460#547..648#743..816#1051..1067#1357..1426#1514..1550#2026..2073#2166..2224#2314..2343#2421..2528#2920..3040#3159..3222#3584..3710#atggcggggagggagaagaggcggcgggtggcggcgctcgacggcgaagagcggcggcggcggcaggaggaagcggcgaccctcctccacaggatcagaggtgcactccccctccctctcgtgatcaatcgcttaaaaaacttctgaatccttcatcaaccgaagtaaacacgcgtccctcgttttttgcgcagggttggtgcgctgggtcgtcgccgaggtcgccgccggccgctccccgaccgtcgcgctccaccgctaccagaactactgctcctccgcctccgccgccgccgcgtccccatggtcggtttccgcgtctcgccctaccctaccatctcatcggccgtttcaaattctcaccccgctgtatgcgcttgcagcgcctgcagctacgacgtccccgtgggcacagatgtactctcgctcctccaccggggctcccacgcctcccggctcagtacgcgtcgttgatttttgtctcaacctgcgtatctacgatcatgttaatgttaatcagtttgggatgttttaatcgatgtgcagatgtgctcctcagggttcttctcgtcgtgcagcaactcctgcagcagaacaagcactgctccaagagggacatctactacatgtacccctccatattccaaggtctgcataggttccatgcctccgcttctgcttttgtttcctgctgtgtgtgacaattggaattctgacacctgtttttgtctaaatactgcagaacaagcggttgttgaccgtgcaatcaatgatatatgcgtactcttcaagtgcagccggcacaatctcaatgtggtatgtaccgcgtaatctgtgttattttccttcagaatcttcagttttgcttctagaagttgcgtgtgcatcacataataagattccactttccagctgctacatcctttgtgcccacatttctgtcattaatcacctggagccttttttttctcaatgctgttttacatcttgaattcatgtgtatgtactgtcataactgactggaatgtgtgctaccttggctgcttgcaggttcctgtggcaaaagggtaggaactctgtcaactatcatagagttggagtgtctgaagatatgtttaactcattctactgtcctattgattgtccatgccaatacttaataagataacttctaaccccttatcttccaagcacttggtctgcttaaaacaaaatctggatgtaaatagtgagatgcttctcttttagcaagggcttatagagcactgtgaagctaccattacagtgctatgctcatatattcttcacctattcatttcccctgttaaaaactcatcaatgtctttattattccagcttggtgatgggctggattagatttttagagggagaaaaggaagtctattgtgtaacgaatgtcaatgctgtaagttctatgagtttcctttaatcactgtaatatgtttctttctttttattgatatggtttgatttttgccttcttcaaatgcaggccttctctatcccagttagcatcgaagcaataaaaggttgttcagaaatttaaagatctaaaattcagtatttatgttactgttaatctgcactgaaaataattcctttacaatttgctgacatggattgcgtgaggggttggaccataatctcaattattcagaattagccaccatcctttgttttgagagaatccactttgatatattctgaatcgaatagctaggattgatctcttagcaaggtgctgttcattagactaaagaataaagtcagtgcttagtatctttacccgtcttgatttaagatatgccagaaaaagactggacaacaagtgaacccacagtttattcttcattttccttacaaatatgcggttgattatacttatatcttttactcactttatgttacagatttcctctcttgttatctgtgaacttcagtgttatccctgatgcctgaattatatctcaaggaaattaagctatcttaggttttctcttgtagatgttgttagtgttgcggactacatacttattgtcgagaaggagacaggtatgaggcttaaagattcctcatgtgcatgtgatacttgtactcttccagttccagccttcctacagttcgcttctgtactctccttgcagtgtttcaacgtttggctaatgacaagttctgtgaaaggaatcgctgcattgtgattacagtacttactaactgctgaatctggtgttatacacaaaatatcttcttgcatttattcttatctagtttgatgttgttctgtctgctcagggaagaggctacccagatattccaacaagaaggcatatactctttgttatacttttctcttttatttttctgaattgatatttcaaatgtaaatactttatttttccagattcttgcgctaccttgttgaacagctgcatttgccagtttactgtttggtggatgcagacccttatggtttcgacattctggctacctacaaatttggttcactggtgggtcatgatttatctaattagctgtttgctttgtatttgctattatgcactatctgagcatacctgttaattaaaaatcacatataaccactttatgcaaatgactactacactttccttatcagtagctcccaatagctggattgacctttatgcattacatttttgtccagtatcaaggcacatttgaatcaaacagtatgccttcagtttgtttggcagctctaagcccttttagcatgtcttgttttcacgatattctgtcccattttgctggtagatttggaccttgcaagtttatttaattttccttgaaagcttggtgtatttatttgtgttaactaactaccttctgcaatgcgcttgtgtaattctcattctatttaagcaattggcatacgatgcaaatttcctgcgtgtgcctgatattcggtggcttggggtcttcacatctgattttgaggattatcgccttccagactgctgcctacttcacttgtcgtctgaaggtgactaaactgagggatataaatgactgacatttctacaactttttcttggaacttataagtaccttgtttgttcttattcaattgtcaacctcatgcttcatgtattctgttgcagacagaaggaaagctgaaggaattctctcaaggtgttacttgcacagggaagccccacaatggaggtaaggttgtacttttcgtacccgtattacaagcaattcatgattatgatgggagtttacaagttagagctcggacttagctagtggaacatttctttgcttttgttttaacagtagttaaagtcatttttttgaagactttcttatgaaaaacaggccagcttactaataaaagctgtactcctaacttagtgccttcccttcagtctcatttccttagtttgaaaaactccactacgcccagtaggtgcaagttttggggatgaattttagtgtaccacttgcctaggaagacttggcccattttatttccagtaactttagccctgaattttttacactgatatactttatttggcaggttggagttagaagccatgttgcaaaagggtgtcaaatttgagattgaggcgttatctgcatgttccatttcctttttatcagaagagtacattcccaagaagatcaaacaaggaagacatatataa</dnaseqindica>|
 
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001186714.1 RefSeq:Os03g0752200]|
 
}}
 
[[Category:Genes]]
 
[[Category:Japonica mRNA]]
 
[[Category:Oryza Sativa Japonica Group]]
 
[[Category:Japonica Genes]]
 
[[Category:Japonica Chromosome 3]]
 
[[Category:Chromosome 3]]
 

Latest revision as of 11:16, 4 November 2016

The rice Os03g0752200 was reported as OsSPO11-1 in 2010 [1] by researchers from the China.

Annotated Information

Gene Symbol

  • Os03g0752200 <=>OsSPO11-1


Function

  • Spo11 is a homolog of a subunit of archaebacterial topoisomerase, which catalyzes DNA double-strand breaks and initiates homologous chromosome recombination.
  • OsSPO11-1 is essential for both homologous chromosomes pairing and crossover formation during meiosis in rice.


Mutation

  • The researchers conducted RT-PCR to confirm that the mutant phenotype of the transgenic plants was caused by the defect of OsSPO11-1 gene. Total RNA was extracted from the prepollinated flowers of both wild type and the completely sterile transgenic plants. RT-PCR analysis showed that OsSPO11-1 transcripts in the transgenic plants were downregulated, suggesting that the sterility was caused by the knockdown of OsSPO11-1 gene in these transgenic plants.


Labs working on this gene

  • Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University.
  • State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,


References

  1. OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice


Structured Information