Difference between revisions of "Os03g0752200"
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| − | The rice '''Os03g0752200''' was reported as ''' | + | The rice '''Os03g0752200''' was reported as '''OsSPO11-1''' in 2010 <ref name="ref1" /> by researchers from the China. |
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==Annotated Information== | ==Annotated Information== | ||
===Gene Symbol=== | ===Gene Symbol=== | ||
| − | *'''Os03g0752200 <=> | + | *'''Os03g0752200 <=>OsSPO11-1''' |
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===Function=== | ===Function=== | ||
| − | + | *Spo11 is a homolog of a subunit of archaebacterial topoisomerase, which catalyzes DNA double-strand breaks and initiates homologous chromosome recombination. | |
| − | + | *OsSPO11-1 is essential for both homologous chromosomes pairing and crossover formation during meiosis in rice. | |
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| − | + | ===Mutation=== | |
| − | + | *The researchers conducted RT-PCR to confirm that the mutant phenotype of the transgenic plants was caused by the defect of OsSPO11-1 gene. Total RNA was extracted from the prepollinated flowers of both wild type and the completely sterile transgenic plants. RT-PCR analysis showed that OsSPO11-1 transcripts in the transgenic plants were downregulated, suggesting that the sterility was caused by the knockdown of OsSPO11-1 gene in these transgenic plants. | |
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==Labs working on this gene== | ==Labs working on this gene== | ||
Latest revision as of 11:16, 4 November 2016
The rice Os03g0752200 was reported as OsSPO11-1 in 2010 [1] by researchers from the China.
Contents
Annotated Information
Gene Symbol
- Os03g0752200 <=>OsSPO11-1
Function
- Spo11 is a homolog of a subunit of archaebacterial topoisomerase, which catalyzes DNA double-strand breaks and initiates homologous chromosome recombination.
- OsSPO11-1 is essential for both homologous chromosomes pairing and crossover formation during meiosis in rice.
Mutation
- The researchers conducted RT-PCR to confirm that the mutant phenotype of the transgenic plants was caused by the defect of OsSPO11-1 gene. Total RNA was extracted from the prepollinated flowers of both wild type and the completely sterile transgenic plants. RT-PCR analysis showed that OsSPO11-1 transcripts in the transgenic plants were downregulated, suggesting that the sterility was caused by the knockdown of OsSPO11-1 gene in these transgenic plants.
Labs working on this gene
- Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University.
- State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,
References
- ↑ OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice
