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| − | Please input one-sentence summary here.
| + | The rice gene Os09g0469400 was reported as '''''isa3''''' in 2011<ref name="ref1" /> by researchers from Japan. |
| | | | |
| | ==Annotated Information== | | ==Annotated Information== |
| | + | ===Gene Symbol=== |
| | + | *'''''Os09g0469400 <=> OsISA3,ISA3 |
| | + | |
| | ===Function=== | | ===Function=== |
| − | Please input function information here.
| + | * '''''ISA3''''' facilitates starch metabolism and affects morphological characteristics of plastids in rice. |
| | | | |
| − | ===Expression=== | + | ===Mutation=== |
| − | Please input expression information here.
| + | * A transposon-inserted rice mutant of isoamylase3 (isa3) contained an increased amount of starch in the leaf blade at the end of the night, indicating that ISA3 plays a role in the degradation of transitory starch during the night. |
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| | + | * Furthermore, chloroplasts in the leaf blade of isa3 seedlings were large and pleomorphic. |
| | + | ===Overexpression=== |
| | + | * Overexpression of '''''ISA3''''' in the sugary1 mutant, which is deficient in ISA1 activity, did not convert water-soluble phytoglycogen to starch granules, indicating that ISA1 and ISA3 are not functionally redundant. |
| | + | ===Expression === |
| | + | * ISA3–green fluorescent protein (GFP) fusion protein expressed under the control of the rice ISA3 promoter was targeted to the amyloplast stroma in the endosperm. |
| | + | [[File:Os09g0469400-1.png|center|thumb|600px|'''Fig. 3 ISA3–GFP is localized in the amyloplast stroma in the rice endosperm.''' '' <ref name="ref1" />.'']] |
| | | | |
| | ===Evolution=== | | ===Evolution=== |
| − | Please input evolution information here.
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| | You can also add sub-section(s) at will. | | You can also add sub-section(s) at will. |
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| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | Please input related labs here.
| + | *Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602 Japan |
| | + | *Rice Quality Research Team, National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, 305-8518, Japan |
| | + | *Present address: Food Resource Division, National Food Research Institute, 2-1-12 Kannondai, Tsukuba, 305-8642 Japan |
| | + | *Present address: National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira, Sapporo, 062-8555 Japan |
| | + | ==References== |
| | + | <references> |
| | + | * <ref name="ref1"> |
| | + | Yun MS, Umemoto T, Kawagoe Y. Rice debranching enzyme isoamylase3 facilitates |
| | + | starch metabolism and affects plastid morphogenesis. Plant Cell Physiol. 2011 |
| | + | Jun;52(6):1068-82. doi: 10.1093/pcp/pcr058. PubMed PMID: 21551159; PubMed Central |
| | + | PMCID: PMC3110883. |
| | + | </ref> |
| | + | </references> |
| | | | |
| − | ==References==
| |
| − | Please input cited references here.
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| | | | |
| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 9]] |
| − | GeneName = Os09g0469400|
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| − | Description = Similar to Isoamylase-type starch debranching enzyme ISO3|
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| − | Version = NM_001069968.1 GI:115479678 GeneID:4347328|
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| − | Length = 11317 bp|
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| − | Definition = Oryza sativa Japonica Group Os09g0469400, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| − | | |
| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|
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| − | AP = Chromosome 9:18511063..18522379|
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| − | CDS = 18511482..18511595,18512009..18512120,18512228..18512289,18512533..18512631,18512732..18512836<br>,18512922..18513001,18514807..18514879,18514973..18515062,18515577..18515639<br>,18516466..18516517,18516866..18516966,18517501..18517600,18518045..18518127<br>|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008402:18511063..18522379
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| − | source=RiceChromosome09
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008402:18511063..18522379
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| − | source=RiceChromosome09
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>atgttttggttcaacctagggaatacactgaactgcaaccatcctgttgtcaaggagctcattcttgacagcttgagacactgggttgaggagtatcacatagatggatttcgatttgaccttgcaagtgttctttgtcgtggaccagatggttgtcctcttgatgcacctccactcatcaaggaaattgccaaagatgctgtattatctagatgtaagatcattgctgaaccttgggattgcggcggcctttatctcgtagggcgtttccctaactgggacaggtgggctgaatggaacggcaaatacagagatgatcttcgaagatttattaagggtgaccctggtatgaagggggtgtttgcgactcgtgtgtctggatctgctgatctctatcaggtgaacgagcggaagccttaccatggtgtaaattttgtgattgcacatgatggatttactttatgtgaccttgtttcttacaacttaaagcacaatgatgctaatggagaaggtggctgtgatggatgcaatgacaactttagctggaactgtggtgttgaaggagaaacaaatgatctgaatgtgttaagtctgcgttcaagacaaatgaaaaacttccatgtagctttaatgatttctcagggcaccccaatgatgttgatgggcgatgaatatggtcatacacgttatgggaacaacaatagctatggacatgatacttgcattaataatttccaatgggaacagttggaacaaagaagagatggccatttcaggtttttctcagagatgataaagtttcgtcacagcaacccaatattaagacgagacaggtttcttaataaaaacgatgtcacctggcacgaggattgttgggagaaccaggaaagcaaatttttggcattcacagtacatgatcacaactctggtggagatatctatttggcattcaatgcacatgactattttgtggacgctgtaattcccccaccaccacaccataaatgttggaaccgtgtggtggataccaacctggaatcaccaaatgatattgtaccagaaggggtgccatttacaggaccaaaatacagaattgctccatactcttccattctgctcaaggcaaagccttag</cdnaseq>|
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| − | AA = <aaseq>MFWFNLGNTLNCNHPVVKELILDSLRHWVEEYHIDGFRFDLASV LCRGPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGK YRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTLCDLVSY NLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQGTPM MLMGDEYGHTRYGNNNSYGHDTCINNFQWEQLEQRRDGHFRFFSEMIKFRHSNPILRR DRFLNKNDVTWHEDCWENQESKFLAFTVHDHNSGGDIYLAFNAHDYFVDAVIPPPPHH KCWNRVVDTNLESPNDIVPEGVPFTGPKYRIAPYSSILLKAKP</aaseq>|
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| − | DNA = <dnaseqindica>10785..10898#10260..10371#10091..10152#9749..9847#9544..9648#9379..9458#7501..7573#7318..7407#6741..6803#5863..5914#5414..5514#4780..4879#4253..4335#gctccgctccaccgcgtgtcccactcccaccacccccgctctcccgtccgtccgcgcgcttccccccatgcgagatcaccgtctcctccgcctcctcctcgttggctcgtcctcttcccccatccgcctctctctctctctctctttctctctctctccagtctcctccactctcctctcttcgagagccgctcgcgacgcgagcctcttggagcgcgcgccctataactaggcacccgtgtccccgtcccggatctcccccccacccgtcgctccgccccgtccccattttggatggattccattggtataaatcgcgccccgctgggttcctcctcctccgccgccgccgtgacagctcgtcgaggcatcgcgctccgcccggctcgccgctccgtcgcgagtacgaaccggtgagcgtgtgtttctccctcgcctccgcatttttccctcggtttttgaggcggcggcattctgattctgcgcatggtgatcctgagtgcagtgtgggtgtggcgacgatcggcttcggggacgcgtctgggctcagagcgtgttctgagaaggcaggtgtctctatctctgttgcgtcgatgcctcgtttccatgttgatttgggtgtaatgatccgagcgtaattggtggctggtgtaggttaggcgatttgattctgtacgaagcaccacggcgagagctcagaacgggaacgctgggagaagcatgaccgaggtatctcaccaagcttgcagttgctggcctttatattatgatttgatccatatgtacgtttttagggatcgcaatgcttgacacaatcatggtatgtttgtatgttcccagctccatgcaaacatgttctgtatctataaactggatcatgccacattttgccatctcttttaattttgcttctgattctaggatgtgccattttgtcttgtttttctaactttgtgttcaccttctcctttccttatttttaattatgtaaccacaggaaagaggatgtacaatgtctgatactgaaatgccatttaaatattcctctggtaaagccttcccattaggagtgtctcaagttgaaggcgggctgaacttcgcacttttctctcagcatgcttcttctgtcattctctgcttaaagcttcctgggaggtatagctttaccttttcctaaaactgttgtgaccttactggtttttctttttctgattatatgtgtacctgtgggttctggtctcttaaagtctcaactatggcaactggcagttttgttgcatgtcatctatctccttgctgtcatctttttatgaacttgctcaacatactttaggtctcatactcttacgagatatggcaaacttgtttttcatgaccttactacacgatattaagcagttgttatgcctttgacatagagtctatgaaattttgaattcatttttcaaatttgacgctcgaaaggaaaactacttttctgtttttcagactaccatatcgtgagttgatttggagtgtaagcctggttgaaaacttgaaataatctcttacagattaacctgtaattttagcttgtcctttccacggtaacatgttacattctctatctaatattctcactctaaactgttcctcaggggaactgaagatgagaaaggtgcagatgttgtcgagtttgttttagaccagcagaagaacaaaactggggatatatggcatgtgatagtggaggtactgtcatagaatttttgtgggtacattttaaagtaagagtatcattctatatacatcgacaaatcactgtggataactttcttgcaacaagttgaattgagtattcatctgatacatattttgattatagttacaatgtttcatattcttcaaagtgaaacttcattttacaatgtgttcatggtatggagcccttccaccgatttgagtcctaaaggactgatttgggtgctcatctcatttatttattgttagaactcatgaccattcttttaaaaatgctcaaatttgagaggaacagtactgaaataatattcctgatttggccaaaacgttggctttgtgatgctaacttgtttatctccactaaaaggaagaagtgctgaatggtaatcttggctagttcattgcctcacttctaggatgctttcacctctgtttgtttgagtgcctgactcaatatttttgtccatctattatatagtagtcatggaaggaataatcttcacctgtttttcttgtatgtaatttgctatgcatatatgctgcatttttttttgttatttctatgcttcattaaaactgtacaaccttaaagaaatcttctctatgacttccagggcttgcctgcttctggtgttctttatgggtatcgtgttggtggccctcaaggatgggaccaaggccatagatttgatagcagcactgttcttctggacccttatgcaaaattagtttctggtcgaaagtactttggggttgctgaagagaagtcaagccagcattttggaacatatgattttgatagctctccttttgattggggtgatgactacaggcttccgaatttgcctgaggtactattatcatttggtaggattgacaagatgttccaaacatgaagttctcaattttttgtttctttaatctaggcagatctagtcatatatgaaatgaacgtccgtgctttcaccgcggatgagtcaagtggccttgattcgacttctcgtggaagctatcttggtctcattgataaagtaagtaccattttttgtttgtttatgaacacaactatttgtttgtatttagaagatttccctattactttcttaaatttcttatttttatcatcgagtttagttaccctgtccactttaagaggctgatttccttcgcatttctgtgactattgcttgtgacttacacatgaaatagttttggtttcatagatccctcatttgctggaacttggcgttaatgcagtggaactacttcctgtttttgagtatgatgagctggaattcaagaggtaccccaacccaagggaccacatggtaaacttatacactttatccatgcttaatatccatcgtagtcaccatcagagtgactttctctcttctcaattgaaaggtcaatacatggggctattctacgataaacttttttgcacccatgagccgttatgctagtgccggtggtggaccagtggctgcttccaaagagctcaagcagatggtcaaagaattgcataaagctggcatagaggtatccaaactcaatatataccagcagattttactgatcattgttaatttcatgcaccgccaggataaaatacatataatggcatcaccattttgtgcaggttattctggatgttgtttacaaccatacaaatgaggcggatgatgctcacccgtatatgacttctttccgtggcattgataacaaggtgaactttctaattgttttgttgccaaaaagagcatctatatgcttccacctgttctttcgaccagactgcacttaggggttaaaaaactaggttagctaattccaatgcagctaggttagcgtagcccgactttttttgttgttcttggcaagttggcaacaccagtcatgccgaacaactgttttggcttgatgacgtggtttacctagttagtttaatcctgtttaggacttaagatgctcaaagtgctttcaccaggttaaattatcacgggcaccacctaaccaccaggttttgttgggttaaatttgcttatcaggtttttgacgaatttatataaaaagatgtcctgttcttgcaggtctattacatgttagacctgaacaaaaatgctgaactactgaacttctcaggctgcggtaaaaccatggttcctgtactcgtatactaatttatttgggactttactctatatcaataactgtttttactgcaatctaatactaattctgcttttgcttaagaccttaaaggatgtaatatatctcagttttacctatggacatccaaataccaaattattaattatattttctgttaagcctttatttatacagctttacagctggttacttttaagaaaaatgctatgatttatttctgaaattactgcttgtgtatcttgctgatctaacatgtttaatgactagatacttacatatcacatgatttgttcaaaattttgaaagtgtaattgtttcacttttaagtcttaacggtatgttttggttcaacctagggaatacactgaactgcaaccatcctgttgtcaaggagctcattcttgacagcttgagacactggtatggacaacttgcagctaagatttttctcataaatccaattgtgtgtagattcatccatatttctttgtttcatcttgactatgtcggcattattcataatcaagtgtcaaaaaatatctatatcgatttgagctttcatgttggtatgatcagaccaagatgatgtatcgacagcctaaaattattcaaaatttcctctaggctatagcacatgcttgtagctatttggcttgtagcaagacagttgatatatgagagatagttcatggctatgtcttattgtgaaccttggaaaacaagatagccagaaactgcaacaaaaacagttatttttatacaaataactgaattatagcatgcatttttattttctctttgacaatggttttgtcatatttcaaatgtcatttctaatctccatgcttgttttaatgcatcaagggttgaggagtatcacatagatggatttcgatttgaccttgcaagtgttctttgtcgt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tcttaataaagtaagtaatacctagaagtgctatcattgcaattaactggtttattgaagacaatcatttcatcatcttctcaaaaaatagctgctggtgtctgtcgaaattccaagtatattttctcagtcaagtctgattagaatagtataatacagcaaatatacactttgcagcttttgcagttgcatctcttagtttttttttcgtctcaaatgttcttcattgtattgtgatcttccttgttggtagaacgatgtcacctggcacgaggattgttgggagaaccaggaaagcaaatttttggcattcacgtaagtttgttgatcactttcgtttatgcaccttattatggtatttgtttatgatgctccaaaatccaaattcttggcttctgactttcattgccattttactgtagagtacatgatcacaactctggtggagatatctatttggcattcaatgcacatgactattttgtggacgctgtaattcccccaccaccacaccataaatgttggaaccgtgtggtatggatacttttcagcattgctaaatttcttacgtatagtaaagtaaattcctacataatcctagcatggtgtggttgtaatttctaaatatgtagaagtgctgccagtactcattctgaattttactacattagacgctcggacaaataccaactcttaaaagaagatatacaagtttccacaaaatttctcaggaatagacataggtagtactgatacagttctacaaccaaaagctgtatacggttgttacttgtgctagcaatcctgctaaaactggtttggagtttattgctcattagctacttttaatttttacaatgattggtctggctggatttgatcactcttttctcacacctttccgctcctattgtcattctaaatctgctctaataatctttttgtaggtggataccaacctggaatcaccaaatgatattgtaccagaaggggtgccatttacaggaccaaaatacagaattgctccatactcttccattctgctcaaggcaaagccttagaaaagttagtagttgagagcataaaaataatgccaccaatgctggcttggttggttaataacaggttgatcacattagcagtctgcacttcaaaaaggcaacaggtatagagaatgtctaataacaactgccgaatcatagttggctttggcagatgaggccacttttggcattcaggaatttcttccagttattactgtaggaatgaatgggctagggcttagggccctctgggggcagcaaagctcgaggtgtcgtctcggtttcatgtgagcgtgacctggctatggtcaccctatcatttatcaaggtcctgtttcgtaataatgtgtcatcaaacactcaactcccatgagaatgtatgccagcatctattttgctggaaattcatgaaaaaaaggaacaatgctattagtccc</dnaseqindica>|
| |
| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069968.1 RefSeq:Os09g0469400]|
| |
| − | }}
| |
| − | [[Category:Genes]] | |
| − | [[Category:Japonica mRNA]]
| |
| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]] | |
| − | [[Category:Japonica Chromosome 9]]
| |
| − | [[Category:Chromosome 9]]
| |
