Difference between revisions of "Os09g0469400"
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| − | The rice gene | + | The rice gene Os09g0469400 was reported as '''''isa3''''' in 2011<ref name="ref1" /> by researchers from Japan. |
==Annotated Information== | ==Annotated Information== | ||
===Gene Symbol=== | ===Gene Symbol=== | ||
| − | *''''' <=> | + | *'''''Os09g0469400 <=> OsISA3,ISA3 |
===Function=== | ===Function=== | ||
| − | + | * '''''ISA3''''' facilitates starch metabolism and affects morphological characteristics of plastids in rice. | |
===Mutation=== | ===Mutation=== | ||
| − | + | * A transposon-inserted rice mutant of isoamylase3 (isa3) contained an increased amount of starch in the leaf blade at the end of the night, indicating that ISA3 plays a role in the degradation of transitory starch during the night. | |
| + | * Furthermore, chloroplasts in the leaf blade of isa3 seedlings were large and pleomorphic. | ||
| + | ===Overexpression=== | ||
| + | * Overexpression of '''''ISA3''''' in the sugary1 mutant, which is deficient in ISA1 activity, did not convert water-soluble phytoglycogen to starch granules, indicating that ISA1 and ISA3 are not functionally redundant. | ||
===Expression === | ===Expression === | ||
| − | [[File:.png| | + | * ISA3–green fluorescent protein (GFP) fusion protein expressed under the control of the rice ISA3 promoter was targeted to the amyloplast stroma in the endosperm. |
| − | + | [[File:Os09g0469400-1.png|center|thumb|600px|'''Fig. 3 ISA3–GFP is localized in the amyloplast stroma in the rice endosperm.''' '' <ref name="ref1" />.'']] | |
===Evolution=== | ===Evolution=== | ||
| Line 19: | Line 22: | ||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | *Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602 Japan | |
| + | *Rice Quality Research Team, National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, 305-8518, Japan | ||
| + | *Present address: Food Resource Division, National Food Research Institute, 2-1-12 Kannondai, Tsukuba, 305-8642 Japan | ||
| + | *Present address: National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira, Sapporo, 062-8555 Japan | ||
==References== | ==References== | ||
<references> | <references> | ||
* <ref name="ref1"> | * <ref name="ref1"> | ||
| − | + | Yun MS, Umemoto T, Kawagoe Y. Rice debranching enzyme isoamylase3 facilitates | |
| + | starch metabolism and affects plastid morphogenesis. Plant Cell Physiol. 2011 | ||
| + | Jun;52(6):1068-82. doi: 10.1093/pcp/pcr058. PubMed PMID: 21551159; PubMed Central | ||
| + | PMCID: PMC3110883. | ||
</ref> | </ref> | ||
</references> | </references> | ||
Latest revision as of 07:06, 20 November 2016
The rice gene Os09g0469400 was reported as isa3 in 2011[1] by researchers from Japan.
Contents
Annotated Information
Gene Symbol
- Os09g0469400 <=> OsISA3,ISA3
Function
- ISA3 facilitates starch metabolism and affects morphological characteristics of plastids in rice.
Mutation
- A transposon-inserted rice mutant of isoamylase3 (isa3) contained an increased amount of starch in the leaf blade at the end of the night, indicating that ISA3 plays a role in the degradation of transitory starch during the night.
- Furthermore, chloroplasts in the leaf blade of isa3 seedlings were large and pleomorphic.
Overexpression
- Overexpression of ISA3 in the sugary1 mutant, which is deficient in ISA1 activity, did not convert water-soluble phytoglycogen to starch granules, indicating that ISA1 and ISA3 are not functionally redundant.
Expression
- ISA3–green fluorescent protein (GFP) fusion protein expressed under the control of the rice ISA3 promoter was targeted to the amyloplast stroma in the endosperm.
Fig. 3 ISA3–GFP is localized in the amyloplast stroma in the rice endosperm. [1].
Evolution
You can also add sub-section(s) at will.
Labs working on this gene
- Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602 Japan
- Rice Quality Research Team, National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, 305-8518, Japan
- Present address: Food Resource Division, National Food Research Institute, 2-1-12 Kannondai, Tsukuba, 305-8642 Japan
- Present address: National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira, Sapporo, 062-8555 Japan
References
