Difference between revisions of "Os08g0430500"
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| − | The rice gene | + | The rice gene Os08g0430500 was reported as '''''GF14c''''' in 2009<ref name="ref1" /> by researchers from Japan. |
==Annotated Information== | ==Annotated Information== | ||
===Gene Symbol=== | ===Gene Symbol=== | ||
| − | *''''' <=> | + | *'''''Os08g0430500 <=> GF14c, OsGF14c |
===Function=== | ===Function=== | ||
| − | [[File:.png|right|thumb| | + | * '''''GF14c''''' encodes an Hd3a-interacting protein. |
| + | * GF14c acts as a negative regulator of flowering by interacting with Hd3a. | ||
| + | ===Mutation=== | ||
| + | [[File:Os08g0430500-2.png|right|thumb|200px|'''Fig. 5 Characterization of gf14c T-DNA insertion plants.''' '' <ref name="ref1" />.'']] | ||
| + | * To study further the function of GF14c in fl owering, we also used the gf14c/GF14c heterozygous T-DNA insertion mutants in japonica rice cv ‘Dongjin’ generated by Postech, Korea. The gf14c/ GF14c plants fl owered earlier than those of the wild type by about 14 d (12–17-d depending on the plants) | ||
| − | + | * GF14c-overexpression plants were delayed by 5–20 d in fl owering relative to wild-type plants | |
| − | |||
===Expression === | ===Expression === | ||
| − | |||
| − | |||
===Evolution=== | ===Evolution=== | ||
| + | [[File:Os08g0430500-1.png|right|thumb|250px|'''Fig. 2 Tissue-specific GF14s family members in rice.''' '' <ref name="ref1" />.'']] | ||
| + | * GF14s belong to the 14-3-3 protein group and have eight isoforms, designated as GF14a–h in rice <ref name="ref2" /><ref name="ref3" />, that share 85–95% amino acid identity ( Fig. 2A ). To determine whether the GF14 genes are expressed in rice organs, we performed semi-quantitative reverse transcription–PCR (RT–PCR) using specifi c primers ( Fig. 2B ). | ||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0101 Japan | |
==References== | ==References== | ||
<references> | <references> | ||
* <ref name="ref1"> | * <ref name="ref1"> | ||
| − | + | Purwestri YA, Ogaki Y, Tamaki S, Tsuji H, Shimamoto K. The 14-3-3 protein | |
| + | GF14c acts as a negative regulator of flowering in rice by interacting with the | ||
| + | florigen Hd3a. Plant Cell Physiol. 2009 Mar;50(3):429-38. doi: | ||
| + | 10.1093/pcp/pcp012. PubMed PMID: 19179350. | ||
| + | </ref> | ||
| + | * <ref name="ref2"> | ||
| + | Chen , F. , Li , Q. , Sun , L. and He , Z. ( 2006a ) The rice 14-3-3 gene family | ||
| + | and its involvement in responses to biotic and abiotic stress. | ||
| + | DNA Res. 13 : 53 – 63 . | ||
| + | </ref> | ||
| + | * <ref name="ref3"> | ||
| + | Yao , Y. , Du , Y. , Jiang , L. and Liu , J. ( 2007 ) Molecular analysis and | ||
| + | expression pattern of the 14-3-3 gene family. J. Biochem. Mol. Biol. | ||
| + | 40 : 349 – 357. | ||
</ref> | </ref> | ||
</references> | </references> | ||
Latest revision as of 12:26, 11 December 2016
The rice gene Os08g0430500 was reported as GF14c in 2009[1] by researchers from Japan.
Contents
Annotated Information
Gene Symbol
- Os08g0430500 <=> GF14c, OsGF14c
Function
- GF14c encodes an Hd3a-interacting protein.
- GF14c acts as a negative regulator of flowering by interacting with Hd3a.
Mutation
Fig. 5 Characterization of gf14c T-DNA insertion plants. [1].
- To study further the function of GF14c in fl owering, we also used the gf14c/GF14c heterozygous T-DNA insertion mutants in japonica rice cv ‘Dongjin’ generated by Postech, Korea. The gf14c/ GF14c plants fl owered earlier than those of the wild type by about 14 d (12–17-d depending on the plants)
- GF14c-overexpression plants were delayed by 5–20 d in fl owering relative to wild-type plants
Expression
Evolution
Fig. 2 Tissue-specific GF14s family members in rice. [1].
- GF14s belong to the 14-3-3 protein group and have eight isoforms, designated as GF14a–h in rice [2][3], that share 85–95% amino acid identity ( Fig. 2A ). To determine whether the GF14 genes are expressed in rice organs, we performed semi-quantitative reverse transcription–PCR (RT–PCR) using specifi c primers ( Fig. 2B ).
You can also add sub-section(s) at will.
Labs working on this gene
- Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0101 Japan
References
- ↑ 1.0 1.1 1.2 Purwestri YA, Ogaki Y, Tamaki S, Tsuji H, Shimamoto K. The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a. Plant Cell Physiol. 2009 Mar;50(3):429-38. doi: 10.1093/pcp/pcp012. PubMed PMID: 19179350.
- ↑ Chen , F. , Li , Q. , Sun , L. and He , Z. ( 2006a ) The rice 14-3-3 gene family and its involvement in responses to biotic and abiotic stress. DNA Res. 13 : 53 – 63 .
- ↑ Yao , Y. , Du , Y. , Jiang , L. and Liu , J. ( 2007 ) Molecular analysis and expression pattern of the 14-3-3 gene family. J. Biochem. Mol. Biol. 40 : 349 – 357.
