Difference between revisions of "Os08g0430500"

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(Created page with "{{JaponicaGene| GeneName = Os08g0430500| Description = Similar to 14-3-3-like protein S94| Version = NM_001068391.1 GI:115476519 GeneID:4345634| Length = 3042 bp| Definit...")
 
(Mutation)
 
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{{JaponicaGene|
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The rice gene Os08g0430500 was reported as '''''GF14c''''' in 2009<ref name="ref1" /> by researchers from Japan.
GeneName = Os08g0430500|
 
Description = Similar to 14-3-3-like protein S94|
 
Version = NM_001068391.1 GI:115476519 GeneID:4345634|
 
Length = 3042 bp|
 
Definition = Oryza sativa Japonica Group Os08g0430500, complete gene.|
 
Source = Oryza sativa Japonica Group
 
  
  ORGANISM  Oryza sativa Japonica Group
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==Annotated Information==
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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===Gene Symbol===
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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*'''''Os08g0430500 <=> GF14c, OsGF14c
            clade; Ehrhartoideae; Oryzeae; Oryza.
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|
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===Function===
Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
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* '''''GF14c''''' encodes an Hd3a-interacting protein.
AP = Chromosome 8:20900592..20903633|
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* GF14c acts as a negative regulator of flowering by interacting with Hd3a.
CDS = 20900919..20900978,20901072..20901188,20901641..20901763,20901947..20902025,20902114..20902505<br>|
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===Mutation===
GCID = <gbrowseImage1>
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[[File:Os08g0430500-2.png|right|thumb|200px|'''Fig. 5 Characterization of gf14c T-DNA insertion plants.''' '' <ref name="ref1" />.'']]
name=NC_008401:20900592..20903633
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* To study further the function of GF14c in fl owering, we also used the gf14c/GF14c heterozygous T-DNA insertion mutants in japonica rice cv ‘Dongjin’ generated by Postech, Korea. The gf14c/ GF14c plants fl owered earlier than those of the wild type by about 14 d (12–17-d depending on the plants)
source=RiceChromosome08
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preset=GeneLocation
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* GF14c-overexpression plants were delayed by 5–20 d in fl owering relative to wild-type plants
</gbrowseImage1>|
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GSID = <gbrowseImage2>
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===Expression ===
name=NC_008401:20900592..20903633
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source=RiceChromosome08
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===Evolution===
preset=GeneLocation
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[[File:Os08g0430500-1.png|right|thumb|250px|'''Fig. 2 Tissue-specific GF14s family members in rice.''' '' <ref name="ref1" />.'']]
</gbrowseImage2>|
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* GF14s belong to the 14-3-3 protein group and have eight isoforms, designated as GF14a–h in rice <ref name="ref2" /><ref name="ref3" />, that share 85–95% amino acid identity ( Fig. 2A ). To determine whether the GF14 genes are expressed in rice organs, we performed semi-quantitative reverse transcription–PCR (RT–PCR) using specifi c primers ( Fig. 2B ).
CDNA = <cdnaseq>atgtctcgggaggagaatgtctacatggccaagctggccgagcaggctgaaaggtatgaggagatggttgagtacatggagaaggttgcaaagactgtagatgtggaagagctcactgttgaggagcgcaacctcttgtctgttgcttacaagaatgtgattggtgcccgccgtgcctcctggcgtattgtctcatccattgaacagaaggaggagggtcgtggcaatgaggaacatgttactctgatcaaggagtaccgtggcaagattgaagctgagctgagcaagatttgcgatggtatcctgaagttgcttgactcacaccttgtgccctcatctactgctgcagaatctaaggtgttttacctcaagatgaagggtgattaccacaggtaccttgcggaatttaagactggtgccgagagaaaggaagctgctgagagcacaatggtggcttacaaggctgctcaggatattgctctggcggatcttgctcccacccatcccataaggcttggactggcacttaacttctctgtgttctactacgagattctaaactctccagacaaggcttgcaaccttgctaagcaggcgtttgacgaagccatctccgagttggataccctcggggaggagtcttacaaggacagcactttgatcatgcagctcctgagggacaacttgaccctctggacctctgacctcacggaggacggtggtgatgaggtgaaagaagcctccaagggcgacgcctgcgagggccagtaa</cdnaseq>|
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AA = <aaseq>MSREENVYMAKLAEQAERYEEMVEYMEKVAKTVDVEELTVEERN                    LLSVAYKNVIGARRASWRIVSSIEQKEEGRGNEEHVTLIKEYRGKIEAELSKICDGIL                    KLLDSHLVPSSTAAESKVFYLKMKGDYHRYLAEFKTGAERKEAAESTMVAYKAAQDIA                    LADLAPTHPIRLGLALNFSVFYYEILNSPDKACNLAKQAFDEAISELDTLGEESYKDS                    TLIMQLLRDNLTLWTSDLTEDGGDEVKEASKGDACEGQ</aaseq>|
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You can also add sub-section(s) at will.
DNA = <dnaseqindica>2656..2715#2446..2562#1871..1993#1609..1687#1129..1520#gccgaacaaaaagcattcgcatccacgagaccactcgaacccgacccgcctcgccgccgccgccaccgaaggttagggttgccggaattttttctcttcctcttcctctcgcggttgcatttctaggcgtgctcgatctcacgggaaggcgtgcggcgatgtttttttttttggcgatttttttggggtggtgggttctggactgtgggatgtggttttgggctagatctggtgtgctcggtggtgatgggtgatttggatgggattggattcgtgagatctgcgatcccgtgcacgcgaggggagtagatccgctcggtttggcgagcttcgtctcgtttagaagtggggatttttgttttttgctttttttggattatcatctctgagttgagcaaacgtctgttggggatttggaaaagttagacagatttatttatttatttatttatttatttatttatttatttatttattgtggaagtatgttgatggtatcggttattagctgcgtttgatccacggattagatctaggtttaatctgttaatagtgtggggatctatatatatgtagcggtgaaaggtgcacgcctgctcggtattggggtgataatttttttatctggattgggaggtaggtggtcttggtttgtggaaaatcagtgtttgtctcgtgggtggattgatgcgacgtttagatctagcctagccatgtttatgatttattgatctgcaatgtttagatatatccctcattataaggctgtaattgagtttctgttagttctttctgtattgaacaatttttagtgattacaaatacaacttagttgagattatgaaatattttgaatttggacatgatattggcttattcatttgttacagcctttaaaaagagttagtattaaatatttgatggaatttgtaggattcagtagtattattcacaattgtttaattattcatttctcaacaaatgaacatccttgatatgaatgggaacatgatctgttttgtacaattataccttctggagagaccccatggctttcagattattaatgttggtgaatgcttatcagtacctttttatctggctacagtaatcccttaattggtcaaaatgtctcgggaggagaatgtctacatggccaagctggccgagcaggctgaaaggtatgaggagatggttgagtacatggagaaggttgcaaagactgtagatgtggaagagctcactgttgaggagcgcaacctcttgtctgttgcttacaagaatgtgattggtgcccgccgtgcctcctggcgtattgtctcatccattgaacagaaggaggagggtcgtggcaatgaggaacatgttactctgatcaaggagtaccgtggcaagattgaagctgagctgagcaagatttgcgatggtatcctgaagttgcttgactcacaccttgtgccctcatctactgctgcagaatctaaggtgttttacctcaagatgaagggtgattaccacaggttgggaatgatgcagcacttatcataaaaacaactgaatccttagtttatattgactattctaatttggttagtttataatttgcaggtaccttgcggaatttaagactggtgccgagagaaaggaagctgctgagagcacaatggtggcttacaaggctgctcaggtaggttgatttattttaattctgagataacatatatgctataccactgattcacttctcattttattactctagcaactaattagtacatgtctccatctggtaataaatgcatttcattgactgcttagtcgattcaggcaatgctttctgaaattgtctgtcccacgtttttctatgtaggatattgctctggcggatcttgctcccacccatcccataaggcttggactggcacttaacttctctgtgttctactacgagattctaaactctccagacaaggcttgcaaccttgctaagcaggttggtggtcatttgctcttctcttcaatataatcatgattgtgtcattagtatgaggtttctgtgctttaggatatgttgtatgatattaatttgaatttatactttgaagttaacttgctggtcattaaactctatagtttcctgctttaacttggatattcttcccctgcttattgttgattaaatttgtatttgtgcacgtggtagtatttgcctagtgatagctcatagtgtggtaattattttaatggggccaaagccaacaaaaaagtaagcatgataaatataggtttgtcaaatctgaacaaactattttgttttatgcattgttgaatcgctggtccttgatgtgtctgtgcttgttgtatgaaccctgacttttttgccatctggacgtttgtcgctatatcaactctaaataaatgtgactgatgaaccgtttgctacaggcgtttgacgaagccatctccgagttggataccctcggggaggagtcttacaaggacagcactttgatcatgcagctcctgagggacaacttgaccctctggacctctgacctcacggtacctctcaacacatcataagctcccttctgcaaaatccatcgacctaatgagcaacttgctaattttaccttgtgaaactatctgtggcaggaggacggtggtgatgaggtgaaagaagcctccaagggcgacgcctgcgagggccagtaaaatgggaagatcgatcgatcgatggctccgcatgttattggagaccatcgatttagatgcctcatgctgctgtcaccatgatggatggattcttctcctgttctactagaatgtttttcttcctgtccccccttcctctctcttctctggtttttactagggtggtagcggtcgaattagttcttcccattgctttgcatttggtgctagtggtccgtctgggctgattgttttcctctggatatgactctcgtgtgtgttgtctccagatagtgttttattgagcaatatttaaagttgtcgtccacctcctcgatgttgtttgtc</dnaseqindica>|
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==Labs working on this gene==
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001068391.1 RefSeq:Os08g0430500]|
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* Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0101 Japan
}}
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==References==
[[Category:Genes]]
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<references>
[[Category:Japonica mRNA]]
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* <ref name="ref1">
[[Category:Oryza Sativa Japonica Group]]
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Purwestri YA, Ogaki Y, Tamaki S, Tsuji H, Shimamoto K. The 14-3-3 protein
[[Category:Japonica Genes]]
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GF14c acts as a negative regulator of flowering in rice by interacting with the
[[Category:Japonica Chromosome 8]]
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florigen Hd3a. Plant Cell Physiol. 2009 Mar;50(3):429-38. doi:
[[Category:Chromosome 8]]
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10.1093/pcp/pcp012. PubMed PMID: 19179350.
 +
</ref>
 +
* <ref name="ref2">
 +
Chen , F. , Li , Q. , Sun , L. and He , Z. ( 2006a ) The rice 14-3-3 gene family
 +
and its involvement in responses to biotic and abiotic stress.
 +
DNA Res. 13 : 53 – 63 .
 +
</ref>
 +
* <ref name="ref3">
 +
Yao , Y. , Du , Y. , Jiang , L. and Liu , J. ( 2007 ) Molecular analysis and
 +
expression pattern of the 14-3-3 gene family. J. Biochem. Mol. Biol.
 +
40 : 349 – 357.
 +
</ref>
 +
</references>
 +
 
 +
 
 +
==Structured Information==
 +
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 8]]

Latest revision as of 12:26, 11 December 2016

The rice gene Os08g0430500 was reported as GF14c in 2009[1] by researchers from Japan.

Annotated Information

Gene Symbol

  • Os08g0430500 <=> GF14c, OsGF14c

Function

  • GF14c encodes an Hd3a-interacting protein.
  • GF14c acts as a negative regulator of flowering by interacting with Hd3a.

Mutation

Fig. 5 Characterization of gf14c T-DNA insertion plants. [1].
  • To study further the function of GF14c in fl owering, we also used the gf14c/GF14c heterozygous T-DNA insertion mutants in japonica rice cv ‘Dongjin’ generated by Postech, Korea. The gf14c/ GF14c plants fl owered earlier than those of the wild type by about 14 d (12–17-d depending on the plants)
  • GF14c-overexpression plants were delayed by 5–20 d in fl owering relative to wild-type plants

Expression

Evolution

Fig. 2 Tissue-specific GF14s family members in rice. [1].
  • GF14s belong to the 14-3-3 protein group and have eight isoforms, designated as GF14a–h in rice [2][3], that share 85–95% amino acid identity ( Fig. 2A ). To determine whether the GF14 genes are expressed in rice organs, we performed semi-quantitative reverse transcription–PCR (RT–PCR) using specifi c primers ( Fig. 2B ).

You can also add sub-section(s) at will.

Labs working on this gene

  • Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0101 Japan

References

  1. 1.0 1.1 1.2 Purwestri YA, Ogaki Y, Tamaki S, Tsuji H, Shimamoto K. The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a. Plant Cell Physiol. 2009 Mar;50(3):429-38. doi: 10.1093/pcp/pcp012. PubMed PMID: 19179350.
  2. Chen , F. , Li , Q. , Sun , L. and He , Z. ( 2006a ) The rice 14-3-3 gene family and its involvement in responses to biotic and abiotic stress. DNA Res. 13 : 53 – 63 .
  3. Yao , Y. , Du , Y. , Jiang , L. and Liu , J. ( 2007 ) Molecular analysis and expression pattern of the 14-3-3 gene family. J. Biochem. Mol. Biol. 40 : 349 – 357.


Structured Information