Difference between revisions of "Os12g0631100"

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(Created page with "{{JaponicaGene| GeneName = Os12g0631100| Description = Ras small GTPase, Ras type family protein| Version = NM_001073872.1 GI:115489705 GeneID:4352862| Length = 3156 bp| ...")
 
 
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{{JaponicaGene|
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The rice gene Os12g0631100 was reported as '''''OsRab5B2''''' in 2010<ref name="ref1" /> by researchers from China.
GeneName = Os12g0631100|
 
Description = Ras small GTPase, Ras type family protein|
 
Version = NM_001073872.1 GI:115489705 GeneID:4352862|
 
Length = 3156 bp|
 
Definition = Oryza sativa Japonica Group Os12g0631100, complete gene.|
 
Source = Oryza sativa Japonica Group
 
  
  ORGANISM  Oryza sativa Japonica Group
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==Annotated Information==
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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===Gene Symbol===
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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*'''''Os12g0631100 <=> glup4(esp8), esp8, glup4, OsRab5B2, Rab5a, OsRab5a, gpa1, GPA1/Rab5a
            clade; Ehrhartoideae; Oryzeae; Oryza.
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|
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===Function===
Chromosome = [[:category:Japonica Chromosome 12|Chromosome 12]]|
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* The gene corresponding to the gpa1 mutation was found to be '''''OsRab5a''''', which encodes a small GTPase.
AP = Chromosome 12:27248490..27251645|
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* '''''OsRab5a''''' plays an essential role in trafficking of storage protein to PBII, possibly as part of its function in organizing the endomembrane system in developing endosperm cells of rice.
CDS = 27248644..27248685,27248949..27249026,27249122..27249289,27250149..27250197,27250399..27250472<br>,27251007..27251100,27251239..27251345|
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===Mutation===
GCID = <gbrowseImage1>
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* '''''gpa1''''' mutant accumulates 57 kDa pro-glutelins in seeds and whose endosperm has a floury appearance.
name=NC_008405:27248490..27251645
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* Transmission electron microscopy analysis showed that the gpa1 endosperm cells have an enlarged ER lumen and a smaller protein body II (PBII), and accumulated three types of newly generated subcellular structures. Moreover, a proportion of glutelins in the gpa1 endosperm cells were not delivered to PBII, and instead were mis-targeted to two of the newly generated structures or secreted.
source=RiceChromosome12
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===Expression ===
preset=GeneLocation
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* '''''OsRab5a''''' is widely expressed in rice plants. Quantitative RT-PCR analysis showed that the highest expression of '''''OsRab5a''''' occurs in roots.
</gbrowseImage1>|
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GSID = <gbrowseImage2>
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===Evolution===
name=NC_008405:27248490..27251645
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source=RiceChromosome12
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You can also add sub-section(s) at will.
preset=GeneLocation
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==Labs working on this gene==
</gbrowseImage2>|
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*State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China,
CDNA = <cdnaseq>atggcggccaaccccggcaacaagatccgcaacgccaagctggttcttcttggagatgtgggcacgggcaagtcgagcctcgttctccggtttgtgaagggccagtttgttgagttccaggagtccaccatcggcgcggccttcttctcgcaaaccttggcggttaacgatgagacggtgaagttcgaaatctgggatactgcagggcaggagaggtatcatagcttggctccgatgtactatcggggtgcggctgccgcgatagttgtctacgacatcacaaatgcggcctctttcacacgtgcaaaaaaatgggttcaagaacttcaagcgcaaggaaacccaaacacgataatggctcttgctggtaacaaggctgatatggtagaggcgaggcaggtgccagcagaagaggcaaagacctacgcacaagagaatggccttttcttcatggaaacatctgcgaaaacggcgatcaatgtgaacgatgtattccacgagatcgcaaagagattgcttcaaggacagcaggctcaagacacaccggctggaatggttctcaaccagagaccagctgagaggatggtgagcagttcttcatgctgctcataa</cdnaseq>|
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*National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China,
AA = <aaseq>MAANPGNKIRNAKLVLLGDVGTGKSSLVLRFVKGQFVEFQESTI                    GAAFFSQTLAVNDETVKFEIWDTAGQERYHSLAPMYYRGAAAAIVVYDITNAASFTRA                    KKWVQELQAQGNPNTIMALAGNKADMVEARQVPAEEAKTYAQENGLFFMETSAKTAIN                    VNDVFHEIAKRLLQGQQAQDTPAGMVLNQRPAERMVSSSSCCS</aaseq>|
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*College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
DNA = <dnaseqindica>155..196#460..537#633..800#1660..1708#1910..1983#2518..2611#2750..2856#aaaaagaattttcaggttttcttctcgctttcaccggagaaggagaaggagaggaaggtgttgatcgaatcctcctccaatcgcgcgcgattcgatccccgttgctggccgctcgctcgctcgatccctcatccgatctgaatcccccgatctgatggcggccaaccccggcaacaagatccgcaacgccaagctggtgaggaatcaaacgtctccctcaatttggtttcttgatttgtttttgtttccccctggatttagggctgatgatatttccccctaagatccctccttgttggtcgcgattagattgatgaactgattcccccttattatgatcgtaaaacaagaactcaatctttacttattggcatcatgtgctcatgttgttcatgtatcacgaattccgggtcttgggagtatgttggtatgcgtttactaacagtgttgattaaccaggttcttcttggagatgtgggcacgggcaagtcgagcctcgttctccggtttgtgaagggccagtttgttgagttccaggtgaatttcttttacacgtgaactactatgacctaagattgagcaggtttggtgtgttgggataggcttgattttgttggttcattcggatgcaggagtccaccatcggcgcggccttcttctcgcaaaccttggcggttaacgatgagacggtgaagttcgaaatctgggatactgcagggcaggagaggtatcatagcttggctccgatgtactatcggggtgcggctgccgcgatagttgtctacgacatcacaaatgcggttagtacaagactaagtgacttgcttgaattttaattaagttgtacatcttgatgatagtttctcaaagttttgatttaatgcaatcatgcctgcatatcactgaaaattataagtacggcaccacagccgcgattggtagaaattactaacagtctaataatataatttacgccattgaaaccagaattacgattagtaagtattggtaaaatcaccccttctctgtgattaacatatcgaaccaagtattcatgataatcgtacaccaactttgacattttaatccatcatcttttattgtcacagaagattcgaacttcctacatatagttttaggttatattgctcttctggtcttggttcaagtgtggccatgttttcccagatgatagactgatgagttggttctgccattaccaattagcaactcccatgtcctggcgttatggcacactgatagtagatcaactagttatcttcactgtttttgcaccttgtttgcatcttatgagcaagccctattcggagaagatactgtatctattgtgtgcccttacttgaacggaggaacccttcaattcacggtcttgcgatcttatgtattagcatattagcaacgcatctctttatatttttcttgcttcacatgcagcttttagtgtgtgttcttggtaaacatgatttcgatctgcattacaagtttttgggaagaaataaatatatcatagttgatagtttacaccgtgcacaaatttaagtctaacttcattcatactgttttatctgagtatgctcactgctcattttgtaaaagtggtgctaatagtggttctcatcattattttctgccttttaggcctctttcacacgtgcaaaaaaatgggttcaagaacttcaagcgcaaggtaatccatttctctttttgtaattttcacgtatgattagaacatctgtaaataagcagctatattgattttgtacaactgctggaccacctgtactgtacagttctcttataagtgaacttgcaatttcatagcactataagacatcgtgtctgtttttttaatagtccagctaatacaagttaacttctttttatacaggaaacccaaacacgataatggctcttgctggtaacaaggctgatatggtagaggcgaggcaggtgccagcagaagtatgtaattttgcttctcaaatccctttttttgtgattatatgtcaaagcactagtgcatttctgcatgaaactatcatgtcactgtgatttgatatttgtcactagcatctcataatggtgcttattttcctcattgcttcgagcaaaagtataatttggccttaccagcttcaaattactgtatttttctcctgtcacatttgacgatgaattatcagtatgttgtgatagtgaatagcacagaataactatgtctactttttgttgctcctgcttcattgaaaaaataactctgaattgaagtatttaacaaaaaaatatataataaaaagttgtgaaggcaagaaggttttctgttctgtggtggtcttttttgtttgctcattgcaacactgatctattaaataatagataccatcttttgtgtttgtggcactatcctgttcttgaaatgaatttcttttccattatgacagcctctgcggcttgctttgttgatgcttatcaaaagctctaatttgtatggtgcaggaggcaaagacctacgcacaagagaatggccttttcttcatggaaacatctgcgaaaacggcgatcaatgtgaacgatgtattccacgagatcggtgagtttagatcacaaatcatttccgcagtggatttctgtacgccgacagagctggttggttatttggtgtacaaaaatgtacacagcctgcttctgctaatttctaagcgaatttgcattcttaatctgatttcagcaaagagattgcttcaaggacagcaggctcaagacacaccggctggaatggttctcaaccagagaccagctgagaggatggtgagcagttcttcatgctgctcataattcaagtgtggagtatgtgacaactacataatgccatggcctgaaatccaggagaagtgtgtatgcatcagagatgcacacaaagcttgtaaagtggctgctgccatcccagctgctgtttcttattttgtcatgttcgttctgtaaatgtgaatactagggtgtcgctgcagaaattcagatgtattactacagtgtatgctcttgcttaagtatttctcgacactgaaacagagtaaagcaaagtctagtcagattcgttttgtttgcttcaattcagatcatgcagtgagcttgctt</dnaseqindica>|
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==References==
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001073872.1 RefSeq:Os12g0631100]|
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<references>
}}
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* <ref name="ref1">
[[Category:Genes]]
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Wang Y, Ren Y, Liu X, Jiang L, Chen L, Han X, Jin M, Liu S, Liu F, Lv J, Zhou
[[Category:Japonica mRNA]]
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K, Su N, Bao Y, Wan J. OsRab5a regulates endomembrane organization and storage
[[Category:Oryza Sativa Japonica Group]]
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protein trafficking in rice endosperm cells. Plant J. 2010 Dec;64(5):812-24. doi:
[[Category:Japonica Genes]]
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10.1111/j.1365-313X.2010.04370.x. PubMed PMID: 21105928.
[[Category:Japonica Chromosome 12]]
+
 
[[Category:Chromosome 12]]
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</ref>
 +
</references>
 +
 
 +
 
 +
==Structured Information==
 +
    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 12]]

Latest revision as of 09:24, 20 December 2016

The rice gene Os12g0631100 was reported as OsRab5B2 in 2010[1] by researchers from China.

Annotated Information

Gene Symbol

  • Os12g0631100 <=> glup4(esp8), esp8, glup4, OsRab5B2, Rab5a, OsRab5a, gpa1, GPA1/Rab5a

Function

  • The gene corresponding to the gpa1 mutation was found to be OsRab5a, which encodes a small GTPase.
  • OsRab5a plays an essential role in trafficking of storage protein to PBII, possibly as part of its function in organizing the endomembrane system in developing endosperm cells of rice.

Mutation

  • gpa1 mutant accumulates 57 kDa pro-glutelins in seeds and whose endosperm has a floury appearance.
  • Transmission electron microscopy analysis showed that the gpa1 endosperm cells have an enlarged ER lumen and a smaller protein body II (PBII), and accumulated three types of newly generated subcellular structures. Moreover, a proportion of glutelins in the gpa1 endosperm cells were not delivered to PBII, and instead were mis-targeted to two of the newly generated structures or secreted.

Expression

  • OsRab5a is widely expressed in rice plants. Quantitative RT-PCR analysis showed that the highest expression of OsRab5a occurs in roots.

Evolution

You can also add sub-section(s) at will.

Labs working on this gene

  • State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China,
  • National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China,
  • College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China

References

  1. Wang Y, Ren Y, Liu X, Jiang L, Chen L, Han X, Jin M, Liu S, Liu F, Lv J, Zhou K, Su N, Bao Y, Wan J. OsRab5a regulates endomembrane organization and storage protein trafficking in rice endosperm cells. Plant J. 2010 Dec;64(5):812-24. doi: 10.1111/j.1365-313X.2010.04370.x. PubMed PMID: 21105928.


Structured Information