Difference between revisions of "Os01g0853400"

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Please input one-sentence summary here.
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The rice '''''Os01g0853400''''' was reported as '''''COI1''''' in 2012 <ref name="ref1" /> by researchers from China.  
  
 
==Annotated Information==
 
==Annotated Information==
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===Gene Symbol===
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*'''''Os01g0853400''''' '''<=>''' '''''COI1,OsCOI1a,OsCOI1,Os_F0728'''''
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===Function===
 
===Function===
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The F-box protein coronatine insensitive 1 (COI1),（The rice genome contains two closely related COI1genes, OsCOI1a(Os01g0853400) and OsCOI1b(Os05g0449500), which share 83% and 82% sequence identity at the DNA and protein levels, respectively.） a component of the SCF E3 ubiquitin ligase, has been identified as a principal component of a receptor of JA in Arabidopsisand other plants, and the JA ZIM domain (JAZ) family proteins are key regulators of JA signaling that repress transcription of JA-responsive genes through interaction with transcription factors, such as MYC2 . This transcriptional repression requires novel interactor of JAZ (NINJA) and TOPLESS corepressor proteins. Bioactive JA, the jasmonoyl-isoleucine conjugate, promotes physical interaction between COI1 and JAZ proteins that results in degradation of JAZs by the 26S proteasome, leading to initiation of JA responses. Therefore, the SCF COI1 –JAZ protein complex acts as a core site of JA perception. As a regulatory loop, JA also activates JAZ gene transcription, leading to thedown-regulation of JA action and the dynamic nature of JA response.
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* The jasmonic acid (JA) pathway plays a key role in plant defense responses against herbivorous insects.
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* CORONATINE INSENSITIVE1 ('''''COI1''''') is an F-box protein essential for all jasmonate responses.
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* '''''OsCOI1''''' is an indispensable signaling component, controlling JA-regulated defense against chewing insect (LF) in rice plants, and COI1 is also required for induction of TrypPI, POD and PPO in rice defense response to LF infestation.
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===Phenotypic analysis===
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* The researchers silenced OsCOI1 in rice plants via RNA interference (RNAi) to determine the role of OsCOI1 in rice defense against rice leaf folder (LF) Cnaphalocrocis medinalis, a chewing insect, and brown planthopper (BPH) Nilaparvata lugens, a phloem-feeding insect.
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* In wild-type rice plants (WT), the transcripts of OsCOI1 were strongly and continuously up-regulated by LF infestation and methyl jasmonate (MeJA) treatment, but not by BPH infestation.  
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* The abundance of trypsin protease inhibitor (TrypPI), and the enzymatic activities of polyphenol oxidase (PPO) and peroxidase (POD) were enhanced in response to both LF and BPH infestation, but the activity of lipoxygenase (LOX) was only induced by LF.
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* The RNAi lines with repressed expression of OsCOI1 showed reduced resistance against LF, but no change against BPH.  
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* Silencing OsCOI1 did not alter LF-induced LOX activity and JA content, but it led to a reduction in the TrypPI content, POD and PPO activity by 62.3%, 48.5% and 27.2%, respectively.  
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* In addition, MeJA- induced TrypPI and POD activity were reduced by 57.2% and 48.2% in OsCOI1 RNAi plants.
  
 
===Expression===
 
===Expression===
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The transcript levels of both OsCOI1a and OsCOI1bwere significantly reduced in these RNAi lines as detected by RNA blot and quantitative RT-PCR (qRT-PCR)
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* The transcripts of '''''OsCOI1''''' were strongly and continuously up-regulated by LF infestation and methyl jasmonate (MeJA) treatment, but not by BPH infestation.
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analyses (Fig. 1 A and B), indicating that OsCOI1 expression was effectively knocked down by RNAi.
 
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[[File:1.png]]
 
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(A) Suppression of OsCOI1a expression in transgenic RNAi lines as shown by RNA blot analysis. A fragment of 825 to 1,244 nt ofOsCOI1 was used as the probe, and 25SrRNAwas used as the loading control.
 
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[[File:2.jpg]]
 
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(B) Suppression ofOsCOI1b expression in transgenic RNAi lines as shown by qRTPCR.
 
  
 
===Evolution===
 
===Evolution===
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The rice genome contains two closely related COI1genes, OsCOI1a(Os01g0853400) and OsCOI1b(Os05g0449500), which share 83% and 82% sequence identity at the DNA and protein levels, respectively.
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* The rice genome contains two closely related COI1 genes, OsCOI1a(Os01g0853400) and OsCOI1b(Os05g0449500), which share 83% and 82% sequence identity at the DNA and protein levels, respectively.
  
 
==Labs working on this gene==
 
==Labs working on this gene==
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Please input related labs here.
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* State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Ministry of Agriculture, South China Agricultural University, Wushan, Guangzhou,People’s Republic of China,
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* Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, Wushan, Guangzhou, People’s Republic of China
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* Plant Protection Institute, Guangdong Academy of Agricultural Sciences of China, Guangzhou, People’s Republic of China,
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* College of Life Sciences, South China Agricultural University, Wushan, Guangzhou, People’s Republic of China
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* Department of Entomology, Texas A&M University, College Station, Texas, United States of America
  
 
==References==
 
==References==
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Please input cited references here.
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<references>
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* <ref name="ref1">
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Ye M, Luo SM, Xie JF, Li YF, Xu T, Liu Y, Song YY, Zhu-Salzman K, Zeng RS.
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silencing COI1 in rice increases susceptibility to chewing insects and impairs
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inducible defense. PLoS One. 2012;7(4):e36214. doi: 10.1371/journal.pone.0036214.
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PubMed PMID: 22558386; PubMed Central PMCID: PMC3338713.
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</ref>
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</references>
  
 
==Structured Information==
 
==Structured Information==
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{{JaponicaGene|
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    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]]
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GeneName = Os01g0853400|
 
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Description = Similar to Coronatine-insensitive protein 1 (F-box/LRR-repeat protein 2) (AtFBL2) (COI-1) (AtCOI1)|
 
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Version = NM_001051366.2 GI:297597978 GeneID:4324818|
 
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Length = 3825 bp|
 
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Definition = Oryza sativa Japonica Group Os01g0853400, complete gene.|
 
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Source = Oryza sativa Japonica Group
 
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  ORGANISM  Oryza sativa Japonica Group
 
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            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
 
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            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
 
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            clade; Ehrhartoideae; Oryzeae; Oryza.
 
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|
 
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Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
 
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AP = Chromosome 1:38502654..38506478|
 
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CDS = 38502654..38502710,38503400..38503953,38505117..38505591,38505672..38506478|
 
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GCID = <gbrowseImage1>
 
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name=NC_008394:38502654..38506478
 
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source=RiceChromosome01
 
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preset=GeneLocation
 
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</gbrowseImage1>|
 
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GSID = <gbrowseImage2>
 
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name=NC_008394:38502654..38506478
 
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source=RiceChromosome01
 
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preset=GeneLocation
 
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</gbrowseImage2>|
 
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CDNA = <cdnaseq>atgcctccgtatgaaacagctagggcagagccagccagcaacaacaagcagcggccgatctccggcgagacggccgctgggagcagccgatccggccccgatccgatgggtggcgaggtgccggagccgcggcggctcaaccgggcgctcagcttcgacgactgggtccccgacgaggcgctgcacctcgtgatgggccacgtcgaggacccgcgggacagggaggcggcgtcgcgggtgtgccgccgctggcaccgcatcgacgcgctcacgcgcaagcacgtcaccgtcgccttctgctacgccgcgcgccccgcgcgcctccgggagcggttcccgcggctcgagtcgctctcgctcaagggcaagccccgcgccgccatgtacgggctcatccccgacgactggggcgcctacgccgcgccatggatcgacgagctcgccgcgccgctcgagtgcctcaaggcgctccacctccgccgcatgaccgtcaccgacgccgacatcgccgcccttgtccgcgcccgcggacacatgctgcaggagctcaagctcgacaagtgcatcggcttctccactgacgccctccgcctcgtcgcccgctcgtgcagatccctgagaactttatttctggaagagtgccatattactgataagggtggtgaatggcttcatgaacttgctgtcaacaattctgttctggtgacactgaacttctacatgactgaactcaaagtggcgccagctgatctagagcttcttgcaaagaattgcaagtcattgatttcattgaagatgagtgagtgtgatctttcagatctgattagtttttttcaaacagccaatgcgctgcaagactttgctggaggagcattctacgaggtaggagagctcaccaagtatgaaaaagttaagttcccacccagattatgcttcttggggcttacctacatgggaacaaatgagatgcctgttatcttccctttttcgatgaaactcaagaaactggacttgcaatacacttttctcacaacagaagatcattgtcagattattgcaaaatgtcccaatctactaattcttgaggtgaggaacgtgataggagatagagggctagaagttgttggtgatacatgcaagaagctacgaagactccgaattgagcggggtgatgatgatccaggtctgcaggaagagcaaggaggagtttctcagctaggcttgacagccgttgctgttggttgccgtgaattggagtacatagctgcctatgtatcggatatcaccaatggggccctggagtctattgggactttctgcaaaaatctatacgactttcggcttgtgctacttgacagagaaagacaggtaacagatctgccacttgacaatggtgtctgtgctctgttaagaaattgcacaaagcttcggaggtttgctctctaccttagaccaggagggctttcagatgatggccttagctacatcggacagtacagtggaaatatccaatacatgctactgggcaatgttggtgaatctgaccatggattgatccgtttcgcagtgggctgcaccaaccttcagaagcttgaattgagaagctgctgcttcagcgagcgagctttgtccctcgctgtactgcagatgccctccctgagatacatatgggtgcaaggatacagagcatctcaaacaggccttgacctcctgctcatggccaggcctttctggaacatcgagtttacacctccgagccctgagagttttaatcatatgacagaagatggagaaccctgtgtggatagccatgctcaggttcttgcctactattcccttgctggaaggaggtctgactgccctcagtgggtgatccccttgcatcctgcgtga</cdnaseq>|
 
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AA = <aaseq>MPPYETARAEPASNNKQRPISGETAAGSSRSGPDPMGGEVPEPR                    RLNRALSFDDWVPDEALHLVMGHVEDPRDREAASRVCRRWHRIDALTRKHVTVAFCYA                    ARPARLRERFPRLESLSLKGKPRAAMYGLIPDDWGAYAAPWIDELAAPLECLKALHLR                    RMTVTDADIAALVRARGHMLQELKLDKCIGFSTDALRLVARSCRSLRTLFLEECHITD                    KGGEWLHELAVNNSVLVTLNFYMTELKVAPADLELLAKNCKSLISLKMSECDLSDLIS                    FFQTANALQDFAGGAFYEVGELTKYEKVKFPPRLCFLGLTYMGTNEMPVIFPFSMKLK                    KLDLQYTFLTTEDHCQIIAKCPNLLILEVRNVIGDRGLEVVGDTCKKLRRLRIERGDD                    DPGLQEEQGGVSQLGLTAVAVGCRELEYIAAYVSDITNGALESIGTFCKNLYDFRLVL                    LDRERQVTDLPLDNGVCALLRNCTKLRRFALYLRPGGLSDDGLSYIGQYSGNIQYMLL                    GNVGESDHGLIRFAVGCTNLQKLELRSCCFSERALSLAVLQMPSLRYIWVQGYRASQT                    GLDLLLMARPFWNIEFTPPSPESFNHMTEDGEPCVDSHAQVLAYYSLAGRRSDCPQWV                    IPLHPA</aaseq>|
 
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DNA = <dnaseqindica>1..57#747..1300#2464..2938#3019..3825#atgcctccgtatgaaacagctagggcagagccagccagcaacaacaagcagcggccggtacgcaataatggaactctccataagccaagcagaggagagagcggtgcggatgcaacagagtactgagagaaaacagagtattttacgcactgttgtcggctagctgtatccgtttgcgtttcagggtaaatccatccatcatcgatccattcatggtggtgggctgctggtggtggtggtggtgctatgctacaggttaatttaggcgatgcttacgtgatgctaacacccagttaacgtggctagggctacgctaagcaaggagcattgaattcgatcgatatgttgggttttttctctttactagtagcatgccatctagtgtgcatcttacgtagtggaatattatcgggcacccaatattcggctcgcacaaagctccgaggacgaggaggaggacgactaatttggcagctcagctcacctgcacggctgcactgtgctgtgcccggtgggcgaagccatttcacccgcgggcttacgtggcccggcaccaccggccgagccgtgcggggaaacaagtaaacgctcgctcgctgcgtctccgatgcccccgtccccgcctggccgctgctgctctctcgctcttctccctccaataattcgcttgccctctggtggctcaaagcccagggaaattgatggagaagaattggggtagccccatgccacctggttcgggctagatctccggcgagacggccgctgggagcagccgatccggccccgatccgatgggtggcgaggtgccggagccgcggcggctcaaccgggcgctcagcttcgacgactgggtccccgacgaggcgctgcacctcgtgatgggccacgtcgaggacccgcgggacagggaggcggcgtcgcgggtgtgccgccgctggcaccgcatcgacgcgctcacgcgcaagcacgtcaccgtcgccttctgctacgccgcgcgccccgcgcgcctccgggagcggttcccgcggctcgagtcgctctcgctcaagggcaagccccgcgccgccatgtacgggctcatccccgacgactggggcgcctacgccgcgccatggatcgacgagctcgccgcgccgctcgagtgcctcaaggcgctccacctccgccgcatgaccgtcaccgacgccgacatcgccgcccttgtccgcgcccgcggacacatgctgcaggagctcaagctcgacaagtgcatcggcttctccactgacgccctccgcctcgtcgcccgctcgtgcaggtaacccaggagttctctctttcttgtactactcatctgtaacagtgattttttttacctccaatacttgcctaagcgaaagttagcagcagcaactgagcagttggtcttttcctcatttagaagttagggttgatctggtggaggtgctaattttgttgttagttggtgttcctaaatggagtaatggatagtagtgacatgttcttagtgttctaacatgggcaagcaaagttgagccaaccttgctcactttctggttcacaaattcctcccccccccccccctcgagaaaggagctgtcacaaattagcttccacagtccactcgtgactggttgaggccataacgcaccgaatttcaacagcaaaatttgtgagcattagatgcgcacaagatttttgcctcaatagatttgtgtggttctttcgtttacctactttcattatttcatctaattgtgtacttgttgagctgggtttgagaagagtccctaatacataaaaacaacttataagaggtagcaagtctggcataagcaatctgctataatttgaggcagacccataacggttggtcatatatcttttaccaggagtaagtactattgtaatggttggattgtacttgaaaaggactaccctgttcctccttagacatatttggaaccaaaatacagttgcaagttggggattatgttgccgccttgccggtcagttatatcatgctccgatttgtgcggcagtatgtcttgtctcatgtttcttgttaactttgcttagtttgactttgagttcgcaacaatgatgtcgcaccaattatccgttaaaaaaaatttataaagaacgcaagaatattttgtgctaaaggtatatatttatttttgagacctaacttgtgaagtctgcatgcatatggagatgtgtggggtggatgaccagtaataccttgtgtggaatgcatgtatgggatgctttgtgatggttggaagtacttcacaagtcactacacatctactatgagttgctatgtttcttgacttaaaatgtgattcctgttctttcctccaatatatatttcttctaacagcaatttcttcatgctcaatgctaagattacatgctatctctgcagtaacatgtcattctaaattatgtcaacagatccctgagaactttatttctggaagagtgccatattactgataagggtggtgaatggcttcatgaacttgctgtcaacaattctgttctggtgacactgaacttctacatgactgaactcaaagtggcgccagctgatctagagcttcttgcaaagaattgcaagtcattgatttcattgaagatgagtgagtgtgatctttcagatctgattagtttttttcaaacagccaatgcgctgcaagactttgctggaggagcattctacgaggtaggagagctcaccaagtatgaaaaagttaagttcccacccagattatgcttcttggggcttacctacatgggaacaaatgagatgcctgttatcttccctttttcgatgaaactcaagaaactggacttgcaatacacttttctcacaacagaagatcattgtcagattattgcaaaatgtcccaatctactaattcttgaggtaatatttcctgtatacaagcattgattaattgtgtttgaattagtatatgccatattacaacgagatgtcctcaacaggtgaggaacgtgataggagatagagggctagaagttgttggtgatacatgcaagaagctacgaagactccgaattgagcggggtgatgatgatccaggtctgcaggaagagcaaggaggagtttctcagctaggcttgacagccgttgctgttggttgccgtgaattggagtacatagctgcctatgtatcggatatcaccaatggggccctggagtctattgggactttctgcaaaaatctatacgactttcggcttgtgctacttgacagagaaagacaggtaacagatctgccacttgacaatggtgtctgtgctctgttaagaaattgcacaaagcttcggaggtttgctctctaccttagaccaggagggctttcagatgatggccttagctacatcggacagtacagtggaaatatccaatacatgctactgggcaatgttggtgaatctgaccatggattgatccgtttcgcagtgggctgcaccaaccttcagaagcttgaattgagaagctgctgcttcagcgagcgagctttgtccctcgctgtactgcagatgccctccctgagatacatatgggtgcaaggatacagagcatctcaaacaggccttgacctcctgctcatggccaggcctttctggaacatcgagtttacacctccgagccctgagagttttaatcatatgacagaagatggagaaccctgtgtggatagccatgctcaggttcttgcctactattcccttgctggaaggaggtctgactgccctcagtgggtgatccccttgcatcctgcgtga</dnaseqindica>|
 
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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001051366.2 RefSeq:Os01g0853400]|
 
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}}
 
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[[Category:Genes]]
 
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[[Category:Japonica mRNA]]
 
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[[Category:Oryza Sativa Japonica Group]]
 
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[[Category:Japonica Genes]]
 
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[[Category:Japonica Chromosome 1]]
 
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[[Category:Chromosome 1]]
 

Latest revision as of 14:37, 14 February 2017

The rice Os01g0853400 was reported as COI1 in 2012 [1] by researchers from China.

Annotated Information

Gene Symbol

  • Os01g0853400 <=> COI1,OsCOI1a,OsCOI1,Os_F0728

Function

  • The jasmonic acid (JA) pathway plays a key role in plant defense responses against herbivorous insects.
  • CORONATINE INSENSITIVE1 (COI1) is an F-box protein essential for all jasmonate responses.
  • OsCOI1 is an indispensable signaling component, controlling JA-regulated defense against chewing insect (LF) in rice plants, and COI1 is also required for induction of TrypPI, POD and PPO in rice defense response to LF infestation.

Phenotypic analysis

  • The researchers silenced OsCOI1 in rice plants via RNA interference (RNAi) to determine the role of OsCOI1 in rice defense against rice leaf folder (LF) Cnaphalocrocis medinalis, a chewing insect, and brown planthopper (BPH) Nilaparvata lugens, a phloem-feeding insect.
  • In wild-type rice plants (WT), the transcripts of OsCOI1 were strongly and continuously up-regulated by LF infestation and methyl jasmonate (MeJA) treatment, but not by BPH infestation.
  • The abundance of trypsin protease inhibitor (TrypPI), and the enzymatic activities of polyphenol oxidase (PPO) and peroxidase (POD) were enhanced in response to both LF and BPH infestation, but the activity of lipoxygenase (LOX) was only induced by LF.
  • The RNAi lines with repressed expression of OsCOI1 showed reduced resistance against LF, but no change against BPH.
  • Silencing OsCOI1 did not alter LF-induced LOX activity and JA content, but it led to a reduction in the TrypPI content, POD and PPO activity by 62.3%, 48.5% and 27.2%, respectively.
  • In addition, MeJA- induced TrypPI and POD activity were reduced by 57.2% and 48.2% in OsCOI1 RNAi plants.

Expression

  • The transcripts of OsCOI1 were strongly and continuously up-regulated by LF infestation and methyl jasmonate (MeJA) treatment, but not by BPH infestation.

Evolution

  • The rice genome contains two closely related COI1 genes, OsCOI1a(Os01g0853400) and OsCOI1b(Os05g0449500), which share 83% and 82% sequence identity at the DNA and protein levels, respectively.

Labs working on this gene

  • State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Ministry of Agriculture, South China Agricultural University, Wushan, Guangzhou,People’s Republic of China,
  • Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, Wushan, Guangzhou, People’s Republic of China
  • Plant Protection Institute, Guangdong Academy of Agricultural Sciences of China, Guangzhou, People’s Republic of China,
  • College of Life Sciences, South China Agricultural University, Wushan, Guangzhou, People’s Republic of China
  • Department of Entomology, Texas A&M University, College Station, Texas, United States of America

References

  1. ↑ Ye M, Luo SM, Xie JF, Li YF, Xu T, Liu Y, Song YY, Zhu-Salzman K, Zeng RS. silencing COI1 in rice increases susceptibility to chewing insects and impairs inducible defense. PLoS One. 2012;7(4):e36214. doi: 10.1371/journal.pone.0036214. PubMed PMID: 22558386; PubMed Central PMCID: PMC3338713.

Structured Information

Retrieved from "https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0853400&oldid=276280"