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| − | Please input one-sentence summary here.
| + | The rice '''''Os05g0365300''''' was reported as '''''DEPG1''''' in 2012 <ref name="ref1" /> by researchers from China. |
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| | ==Annotated Information== | | ==Annotated Information== |
| | + | [[File:Os05g0365300_Figure3.jpg|right|thumb|300px|'''Figure 3.''' ''Phylogenetic analysis of DEPG1 with the 18 NBS-LRR R genes based on their amino acid sequences.<ref name="ref1" />.'']] |
| | + | [[File:Os05g0365300_Figure5.jpg|right|thumb|300px|'''Figure 5.''' ''Histological analysis of DEPG1 expression in transgenic plants.<ref name="ref1" />.'']] |
| | + | ===Gene Symbol=== |
| | + | *'''''Os05g0365300''''' '''''<=>''''' '''''DEPG1''''','''''OsRP1L1''''','''''OsBBR1''''','''''rNBS33''''' |
| | ===Function=== | | ===Function=== |
| − | Please input function information here.
| + | *DEPG1 is a nucleotide-binding site (NBS)-leucine rich repeat (LRR) gene, and the deduced protein sequence of DEPG1 has approximately 64% identity with that of the disease resistance gene Pi37. |
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| | ===Expression=== | | ===Expression=== |
| − | Please input expression information here.
| + | *Semi-quantitative PCR assays showed that DEPG1 is widely expressed in rice, and is preferentially expressed in internodes, leaf blades, leaf sheaths and flag leaves. |
| | + | *Observation of cross sections of leaves from the transgenic plants with a DEPG1-promoter::glucuronidase (GUS) fusion gene revealed that DEPG1 is also highly expressed in mesophyll tissues where Xoc mainly colonizes. |
| | + | *Xoc negatively regulates expression of DEPG1 at the early stage of the pathogen infection, and so do the three defense-signal compounds including salicylic acid (SA), methyl jasmonate (MeJA) and 1-aminocyclopropane-1-carboxylic-acid (ACC). |
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| | + | ===Phenotypic Analysis=== |
| | + | *Transgenic rice plants overexpressing DEPG1 exhibit enhanced susceptibility to Xoc compared to the wild-type controls. |
| | + | *Enhanced susceptibility to Xoc may be mediated by inhibition of the expression of some SA biosynthesis-related genes and pathogenesis-related genes that may contribute to the disease resistance. |
| | + | *DEPG1 plays roles in the interactions between rice and BLS pathogen Xoc. |
| | + | |
| | + | ===Subcellular Location=== |
| | + | *DEPG1 protein is located to the cytoplasm, which was confirmed by transient expression of DEPG1-GFP (green fluorescent protein) fusion construct in onion epidermal cells. |
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| | ===Evolution=== | | ===Evolution=== |
| − | Please input evolution information here.
| + | *Phylogenetic analysis of DEPG1 and the 18 characterized NBS-LRR genes revealed that DEPG1 is more closely related to Pi37. |
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| | You can also add sub-section(s) at will. | | You can also add sub-section(s) at will. |
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| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | Please input related labs here.
| + | *Xiamen Key Laboratory for Plant Genetics, School of Life Sciences, Xiamen University, Xiamen, 361005, People's Republic of China. |
| | | | |
| | ==References== | | ==References== |
| − | Please input cited references here.
| + | <references> |
| | + | * <ref name="ref1"> |
| | + | Guo L, Li M, Wang W, Wang L, Hao G, Guo C, Chen L. Over-expression in the nucleotide-binding site-leucine rich repeat gene DEPG1 increases susceptibility to bacterial leaf streak disease in transgenic rice plants. Mol Biol Rep. 2012 Apr;39(4):3491-504. doi: 10.1007/s11033-011-1122-6. Epub 2011 Jun 30. PubMed PMID: 21717056. |
| | + | </ref> |
| | + | </references> |
| | + | |
| | | | |
| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 5]] |
| − | GeneName = Os05g0365300|
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| − | Description = Similar to NBS-LRR protein (Fragment)|
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| − | Version = NM_001061860.1 GI:115463450 GeneID:4338554|
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| − | Length = 5170 bp|
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| − | Definition = Oryza sativa Japonica Group Os05g0365300, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| − | | |
| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|
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| − | AP = Chromosome 5:17519575..17524744|
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| − | CDS = 17521477..17524107|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008398:17519575..17524744
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| − | source=RiceChromosome05
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008398:17519575..17524744
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| − | source=RiceChromosome05
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>atggcggaagtggcattggctagcttacgattggcagcatcaccgatcctgaacaagctcctcgctgacgcttcaacataccttggagtggacatggcgagtgagctccgtgaactggagaccagcatcatgccgcagttcgagctgttgattgaagaagctgagaagggcaaccacagggccaagctggacaaatggatacgggagctcaaagaagccttgtacaatgccgaggacctgctggatgagcatgagtacgacatcctcaagcgcaaggtgaagaacggaggcgaggatccttctccagatctagagcatgcctcctccatcggttccatcattaagaaacctatgcgcgcagcatcaagcagcttgtccaatctgcgaccgaagaacataaagcttgtacgccagctgaaggagctcaaggccatcctagcgaaagcaagggacttccgtgaaatgcttggcttaccggctggtagcagtgtagagggggcacaaactggacatacaaaaacagtggtcgttactgcagcgacatcaaccccaccaccaaaggtgtttggtcgcgacgcggaccgcgatcgcattgttgatcttcttacccaacacaagacttgtgctgaggctagccgtttcgttgtctccattgttggacctggaggcatggggaaatccaccttggctcagtatgtttacaatgacaagacgatacaagaacattttgacgtcacgatgtgggtttgcatctcacgcaaacttgatgtccatcgtcatacaagggagatcatcgagtctgcaacaaaagagaagtgtcaaagggttggtaatatggacgttctccagtacaaactaaaagaaatactgcagaaaaaagagaaagtcctgcttgtgttggacgacatttggttcgataaatcccaggacgttgaggaatgggacctgctacttgctcccatactatcttctcagaatggggcaaccaaagttctggtaacttctcgatctaagacacttccacctgctcttttctctgaagatgtcattgatttggaaaatatgaaagatactgagttccaggcactcttcaaacaccatgctttctctggagccacaattcgagacctccagatgtgtggctggttcgaagagcatgcagtgaagattactgaaaggcttggaagatctcctttggcagcaaaagttgtaggttccaacttgaaaagggtgatgaatatcgatgattggaaaggtgctctcactataaagatagacaatttaagtgaacctaagagagctctgttgtggagttatcagaaattagatccatgtcttcagagatgctttctgtattgcagtttatttccaaaaggttacaaatacataattgacgagttggttcacctttgggttgcagagggatttattgatgcacgtgatacgaataaaagaatggaagataccggcatggattacttcaaagagatggtctctggatcctttttccaaccattttctgaaagatttgatagtacggtgtacattatgcatgacctccttcatgatttggcagagtcactctctagagaagactgcttcagattagaagatgacaaggtgagagaaataccatgcactgttcggcatttatctgttcgtgttgagagtattatacaacataagccaagtgtctgcaagctacaacatttgcgaactcttatctgcattgacccactagtggatgttgggagcaatattttcgagcaggtagtattgaatctgaagaagttacaagtactttatttgtcgttttacaacactagaaagttgccagaatcaatcggtcagctaaagcatcttcggtatttgaacatcaagaagacattgatttctgaactaccaaaatcattatgcgatctttaccatttagagttactttatttgcgtccgaaatcgaggttacctgacaaactctgcaatttatgtaagttacgacatcttcaaatgtactctgatggattggagctgtcacggattcctgacataggccggctaactttgcttcaacgtattgatagttttcacgtcctaaaacagaagggacatgagttgcgacagctcaggaacatgaacgaaattggtgggtatttatcgctcagaaatcttgagaatgtgattggcaaggatgaagctttagagtcgaagctgtatcagaaaagtcgtcttgaaggattgacgcttgagtggaatgatgcgaataacatgaatccagagaattgtttacatgttgagattcttgaaggcctcgtgccaccaccacagttggagcacttatcaattagaggttacaaatcgactacctatccaagctggttactcgagggttcccagcttgagaatctagaatcctttgctctttataactgcagcgctttagaaaggctaccatccaataccaagctctttaggcgttgccgtgaattgagccttaagaatctcccgaatatgaaggaactatcatttcttccagcaggtctcacaacgttgtcaattcgtaggtgcccactgcttctcttcgttaccaatgatgaactggagtatcatgatcatagtgagcatatccactag</cdnaseq>|
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| − | AA = <aaseq>MAEVALASLRLAASPILNKLLADASTYLGVDMASELRELETSIM PQFELLIEEAEKGNHRAKLDKWIRELKEALYNAEDLLDEHEYDILKRKVKNGGEDPSP DLEHASSIGSIIKKPMRAASSSLSNLRPKNIKLVRQLKELKAILAKARDFREMLGLPA GSSVEGAQTGHTKTVVVTAATSTPPPKVFGRDADRDRIVDLLTQHKTCAEASRFVVSI VGPGGMGKSTLAQYVYNDKTIQEHFDVTMWVCISRKLDVHRHTREIIESATKEKCQRV GNMDVLQYKLKEILQKKEKVLLVLDDIWFDKSQDVEEWDLLLAPILSSQNGATKVLVT SRSKTLPPALFSEDVIDLENMKDTEFQALFKHHAFSGATIRDLQMCGWFEEHAVKITE RLGRSPLAAKVVGSNLKRVMNIDDWKGALTIKIDNLSEPKRALLWSYQKLDPCLQRCF LYCSLFPKGYKYIIDELVHLWVAEGFIDARDTNKRMEDTGMDYFKEMVSGSFFQPFSE RFDSTVYIMHDLLHDLAESLSREDCFRLEDDKVREIPCTVRHLSVRVESIIQHKPSVC KLQHLRTLICIDPLVDVGSNIFEQVVLNLKKLQVLYLSFYNTRKLPESIGQLKHLRYL NIKKTLISELPKSLCDLYHLELLYLRPKSRLPDKLCNLCKLRHLQMYSDGLELSRIPD IGRLTLLQRIDSFHVLKQKGHELRQLRNMNEIGGYLSLRNLENVIGKDEALESKLYQK SRLEGLTLEWNDANNMNPENCLHVEILEGLVPPPQLEHLSIRGYKSTTYPSWLLEGSQ LENLESFALYNCSALERLPSNTKLFRRCRELSLKNLPNMKELSFLPAGLTTLSIRRCP LLLFVTNDELEYHDHSEHIH</aaseq>|
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| − | DNA = <dnaseqindica>638..3268#gactcctatcccaattcctcttgccacacaccacttatccgctgcagcaacctcgcaatcccctcggcgctcgctggttccagccaagctgcttccgtcctccttcacttcatctgtgcaacagggtaaagcttccagatcttgctttgctgtttttttttattagttcatatccatcttattctactacaaaaatacttgatctgtctccggtgccgttcactgatttcgttttttctttgttgttgtttctcgatgattttaggttattcatccggccctggtggttttgccgtctgcaaacacctgtcttccattcgcatttcagaagaaaaagaaacacacacacaaaaaaactactcgtcagagttttcttcctaatttcccatccaaaaccagtagttttcattgaaaacaaaatcatttgttgttgtactgtccccgtattttgttcgtagcaattcccatccaaaaaacaaaatcatcctagctttttactttcaccttgttcccatcggtccttgtttagatcttcctagttctaagtttaccaacatttctccatcttcacatccaatatctctgtacctaccaaaattacaaagagagagaacagggagacagcaaattcaatccgatggcggaagtggcattggctagcttacgattggcagcatcaccgatcctgaacaagctcctcgctgacgcttcaacataccttggagtggacatggcgagtgagctccgtgaactggagaccagcatcatgccgcagttcgagctgttgattgaagaagctgagaagggcaaccacagggccaagctggacaaatggatacgggagctcaaagaagccttgtacaatgccgaggacctgctggatgagcatgagtacgacatcctcaagcgcaaggtgaagaacggaggcgaggatccttctccagatctagagcatgcctcctccatcggttccatcattaagaaacctatgcgcgcagcatcaagcagcttgtccaatctgcgaccgaagaacataaagcttgtacgccagctgaaggagctcaaggccatcctagcgaaagcaagggacttccgtgaaatgcttggcttaccggctggtagcagtgtagagggggcacaaactggacatacaaaaacagtggtcgttactgcagcgacatcaaccccaccaccaaaggtgtttggtcgcgacgcggaccgcgatcgcattgttgatcttcttacccaacacaagacttgtgctgaggctagccgtttcgttgtctccattgttggacctggaggcatggggaaatccaccttggctcagtatgtttacaatgacaagacgatacaagaacattttgacgtcacgatgtgggtttgcatctcacgcaaacttgatgtccatcgtcatacaagggagatcatcgagtctgcaacaaaagagaagtgtcaaagggttggtaatatggacgttctccagtacaaactaaaagaaatactgcagaaaaaagagaaagtcctgcttgtgttggacgacatttggttcgataaatcccaggacgttgaggaatgggacctgctacttgctcccatactatcttctcagaatggggcaaccaaagttctggtaacttctcgatctaagacacttccacctgctcttttctctgaagatgtcattgatttggaaaatatgaaagatactgagttccaggcactcttcaaacaccatgctttctctggagccacaattcgagacctccagatgtgtggctggttcgaagagcatgcagtgaagattactgaaaggcttggaagatctcctttggcagcaaaagttgtaggttccaacttgaaaagggtgatgaatatcgatgattggaaaggtgctctcactataaagatagacaatttaagtgaacctaagagagctctgttgtggagttatcagaaattagatccatgtcttcagagatgctttctgtattgcagtttatttccaaaaggttacaaatacataattgacgagttggttcacctttgggttgcagagggatttattgatgcacgtgatacgaataaaagaatggaagataccggcatggattacttcaaagagatggtctctggatcctttttccaaccattttctgaaagatttgatagtacggtgtacattatgcatgacctccttcatgatttggcagagtcactctctagagaagactgcttcagattagaagatgacaaggtgagagaaataccatgcactgttcggcatttatctgttcgtgttgagagtattatacaacataagccaagtgtctgcaagctacaacatttgcgaactcttatctgcattgacccactagtggatgttgggagcaatattttcgagcaggtagtattgaatctgaagaagttacaagtactttatttgtcgttttacaacactagaaagttgccagaatcaatcggtcagctaaagcatcttcggtatttgaacatcaagaagacattgatttctgaactaccaaaatcattatgcgatctttaccatttagagttactttatttgcgtccgaaatcgaggttacctgacaaactctgcaatttatgtaagttacgacatcttcaaatgtactctgatggattggagctgtcacggattcctgacataggccggctaactttgcttcaacgtattgatagttttcacgtcctaaaacagaagggacatgagttgcgacagctcaggaacatgaacgaaattggtgggtatttatcgctcagaaatcttgagaatgtgattggcaaggatgaagctttagagtcgaagctgtatcagaaaagtcgtcttgaaggattgacgcttgagtggaatgatgcgaataacatgaatccagagaattgtttacatgttgagattcttgaaggcctcgtgccaccaccacagttggagcacttatcaattagaggttacaaatcgactacctatccaagctggttactcgagggttcccagcttgagaatctagaatcctttgctctttataactgcagcgctttagaaaggctaccatccaataccaagctctttaggcgttgccgtgaattgagccttaagaatctcccgaatatgaaggaactatcatttcttccagcaggtctcacaacgttgtcaattcgtaggtgcccactgcttctcttcgttaccaatgatgaactggagtatcatgatcatagtgagcatatccactaggacagagcaactggttgcacaatttgctttggttggggtgatgggcccatttttagatgcactgtcatcggatcattcatctatgaagcagttggcagcattgatggactctgatatatcaaaaaatcttcaaaccattgaacgcgctctagaaagagaagatgaagtagtgatgaccaaggatgtcatcaaggcatggatgcgttgccacgagcagaggatgagactcatttatgcaaggaggattgggctaccattggtcccaccgtcaggacttagtgatctctcccttaaatcatgcacaattacagatacagctctatccatatgccttggtggcctagcttctctgagatgtttgtccttatccaagatcatgtctttaactactcttccgtcagaagaggtcctgaaaaaattgacaaagcttgattgtttgatcattgatgcctgcttgtttctcggatcgttggggggcttgcgagctgctacttccctttcgcatttaaggttgaactcttgccctgctttagagttggcacacggtgcagaattcatgccagcgtcccttaagagactcgcaataagttgttgtgtgcttgcacccgacttgttctgtggccactggccgcatctgaaagatattttcatacacgactgcagaagctctgtgtcgttgtttgttggtgatcttagctccctgaaagaattcacactttatcatttgccagatttatgtgtgcttgaaggcctgtcttcccttcaacttcacagcgtgtgtttggtagatatcccgaagcttactgctgagtgtgtgtcaaagtttcgtgtccaggatttactgcatgttagcagctctgcggtgttgaacaacatcatctcggctgaaggtatcacagttcagcgtctctcatccttcaaaggtgcaaggagccatccgtttcatttgaagaaactataaattcctcatctgtcaagcagctgcgattagaaagttgtcaaatgagttccctgcagggaaatctgaagtgcctctccagactggaggaacttgacatcttcagttgccccaatatatcatgtttgccagatctcccatcctcccttcagcgcatatccatagtggactgccccaatatatcatctttgccagatctcccatcctccctgcagcacatatacatacgtgactgtccactactgaaggagagctgtagagtacccgatggggaaagctggccaaagattgcgcatattcgttggaagcgtattgactgaattatgcttcagattcacaagaacaaaggaacagaaaggtaacggtttcatcaatgcattatgaaaatataaatttatgcaagatcatttccatccattattgttcgtctaaatacttctttcggttaccagttaaatcgctctcttgtcctctggcagtgctgttccttcgccccctgcattgcagacctccagagtctcacacagctgtttggctatgaacgagagggaccggttcgtattgactgatcggtctgagcttcttcttcttattcttgtgtggttgcatttcgctggctgctggctatggtctatatgcctatggactattaccgagatgtatctgtgtatttggatcttgctctgttatgtttcctgtttgttcttttggactgaattgcttgtagtagctgttatctatggtgtgcaacgtctactgtattactgcaccggtgaagctttgtatgggagtaccatggtgcttttgaactcaggctactgttatcctctcttggagtgtttccttttggacttggtttctattgagtattgactgtaagcctgtaactattatctcttgtatgatgagatgcaagataaggcaaacctgcaatagctctagtc</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001061860.1 RefSeq:Os05g0365300]|
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| − | }}
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| − | [[Category:Genes]] | |
| − | [[Category:Japonica mRNA]]
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| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]] | |
| − | [[Category:Japonica Chromosome 5]]
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| − | [[Category:Chromosome 5]]
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