Difference between revisions of "Os04g0659100"
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===Function=== | ===Function=== | ||
* The rice '''''OsGS2''''' can encode chloroplastic glutamine synthetase proteins. | * The rice '''''OsGS2''''' can encode chloroplastic glutamine synthetase proteins. | ||
| − | * The rice '''''OsGS2''''' is a key enzyme of assimilation process which catalyze glutamate and ammonia into glutamine at the existence of ATP. It is the first reaction of nitrogen assimilation. GS2 could catalyze assimilation of amino produced by chloroplasts photorespiration. | + | * The rice '''''OsGS2''''' is a key enzyme of assimilation process which catalyze glutamate and ammonia into glutamine at the existence of ATP. |
| + | * It is the first reaction of nitrogen assimilation. GS2 could catalyze assimilation of amino produced by chloroplasts photorespiration. | ||
| + | * '''''GS2''''' might be involved in assimilation of ammonium derived from many sources such as nitrate in the soil, atmospheric N 2 , and endogenous amino acids as well as from photorespiration | ||
===Phenotypic analysis=== | ===Phenotypic analysis=== | ||
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* The intracellular contents of NH 4 + and Na + in the stressed plants correlated well with the levels of GS2. | * The intracellular contents of NH 4 + and Na + in the stressed plants correlated well with the levels of GS2. | ||
* Thus, the enhancement of photorespiration conferred resistance to salt in rice plants. | * Thus, the enhancement of photorespiration conferred resistance to salt in rice plants. | ||
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==Labs working on this gene== | ==Labs working on this gene== | ||
Revision as of 02:47, 8 March 2017
The rice Os04g0659100 was reported as OsGS2 in 2000 [1] by researchers from Japan.
Contents
Annotated Information
Function
- The rice OsGS2 can encode chloroplastic glutamine synthetase proteins.
- The rice OsGS2 is a key enzyme of assimilation process which catalyze glutamate and ammonia into glutamine at the existence of ATP.
- It is the first reaction of nitrogen assimilation. GS2 could catalyze assimilation of amino produced by chloroplasts photorespiration.
- GS2 might be involved in assimilation of ammonium derived from many sources such as nitrate in the soil, atmospheric N 2 , and endogenous amino acids as well as from photorespiration
Phenotypic analysis
- Each transgenic rice plant line showed a different accumulation level of GS2.
- A transgenic plant line, G39-2, which accumulated about 1.5-fold more GS2 than the control plant, had an increased photorespiration capacity.
- In another line, G241-12, GS2 was almost lost and photorespiration activity could not be detected.
- Fluorescence quenching analysis revealed that photorespiration could prevent the over-reduction of electron transport systems.
- When exposed to 150 mM NaCl for 2 weeks, the control rice plants completely lost photosystem II activity, but G39-2 plants retained more than 90% activity after the 2-week treatment, whereas G241-12 plants lost these activities within one week. In the presence of isonicotinic acid hydrazide, an inhibitor of photorespiration, G39-2 showed the same salt tolerance as the control plants.
- The intracellular contents of NH 4 + and Na + in the stressed plants correlated well with the levels of GS2.
- Thus, the enhancement of photorespiration conferred resistance to salt in rice plants.
You can also add sub-section(s) at will.
Labs working on this gene
- Research Institute, Meijo University, Nagoya, Aichi, Japan.
- Department of Chemistry, Faculty of Science and Technology, Meijo University, Tenpaku-ku, Nagoya, Aichi, 468-8502 Japan
- Plantech Research Institute, Aoba-ku, Yokohama, Kanagawa, 227-0033 Japan
- Graduate School of Bioagricultral Science, Nagoya University, Chikusa-ku, Nagoya, Aichi, 464-8601 Japan
References
- ↑ Hoshida H, Tanaka Y, Hibino T, Hayashi Y, Tanaka A, Takabe T, Takabe T. Enhanced tolerance to salt stress in transgenic rice that overexpresses chloroplast glutamine synthetase. Plant Mol Biol. 2000 May;43(1):103-11. PubMed PMID: 10949377.
