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| − | Please input one-sentence summary here.
| + | The rice '''''Os08g0445700''''' was reported as '''''OsTPS1''''' in 2011 <ref name="ref1" /> by researchers from China. |
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| | ==Annotated Information== | | ==Annotated Information== |
| | ===Function=== | | ===Function=== |
| − |
| + | * Trehalose-6-phosphate synthase (TPS) is a key enzyme for trehalose biosynthesis. |
| − | Trehalose-6-phosphate synthase (TPS) is a key enzyme for trehalose biosynthesis.Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms. In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). The genome of rice (Oryza sativa) contains 11 OsTPS genes, and only OsTPS1 shows TPS activity. To understand the function of the TPS gene, we identified the T-DNA insertion in a TPSgene of rice. Line 1C-071-05 had T-DNA inserted at the second intron of OsTPS1. Its deduced OsTPS1 protein shared 52% homology with Arabidopsis TPS. Reverse transcriptase-PCR analysis of OsTPS1 showed that OsTPS1 is inducible by drought, salt, cold, and ABA. Leaves of OsTPS1 knockout (KO) plants were more sensitive to drought or cold stress than were the wild types. Furthermore, transgenic rice of UBI::CBF1 had high expression OsTPS1 mRNA, suggesting that OsTPS1 is regulated by the CBF/DREB transcription factor.OsTPS1 overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant phenotypic changes | + | * Overexpression of the trehalose-6-phosphate synthase gene '''''OsTPS1''''' enhances abiotic stress tolerance in rice |
| | + | * Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms. |
| | + | * In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). The genome of rice (Oryza sativa) contains 11 OsTPS genes, and only OsTPS1 shows TPS activity. |
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| | + | ===Phenotypic analysis=== |
| | + | * Leaves of OsTPS1 knockout (KO) plants were more sensitive to drought or cold stress than were the wild types. |
| | + | * Furthermore, transgenic rice of UBI::CBF1 had high expression OsTPS1 mRNA, suggesting that '''''OsTPS1''''' is regulated by the CBF/DREB transcription factor. |
| | + | * '''''OsTPS1''''' overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant phenotypic changes |
| | + | |
| | ===Expression=== | | ===Expression=== |
| − | Please input expression information here.
| + | * Reverse transcriptase-PCR analysis of OsTPS1 showed that OsTPS1 is inducible by drought, salt, cold, and ABA. |
| − | | |
| − | ===Evolution===
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| − | Please input evolution information here.
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| | You can also add sub-section(s) at will. | | You can also add sub-section(s) at will. |
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| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | Please input related labs here.
| + | * Key Laboratory of Gene Engineering Drug and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, 100875, People's Republic of China. |
| | | | |
| | ==References== | | ==References== |
| − | Please input cited references here.
| + | <references> |
| | + | * <ref name="ref1"> |
| | + | Li HW, Zang BS, Deng XW, Wang XP. Overexpression of the trehalose-6-phosphate |
| | + | synthase gene OsTPS1 enhances abiotic stress tolerance in rice. Planta. 2011 |
| | + | Nov;234(5):1007-18. doi: 10.1007/s00425-011-1458-0. PubMed PMID: 21698458. |
| | + | </ref> |
| | + | </references> |
| | | | |
| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
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| − | GeneName = Os08g0445700|
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| − | Description = HAD-superfamily hydrolase subfamily IIB protein|
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| − | Version = NM_001068460.1 GI:115476657 GeneID:4345706|
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| − | Length = 3841 bp|
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| − | Definition = Oryza sativa Japonica Group Os08g0445700, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
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| − | AP = Chromosome 8:21810273..21814113|
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| − | CDS = 21810324..21812209,21812788..21813061,21813512..21813826|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008401:21810273..21814113
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| − | source=RiceChromosome08
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008401:21810273..21814113
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| − | source=RiceChromosome08
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>atgccctccctccccaactccggcgacgagggcggcgccccgcctccgactccgccgccgccgggggcgcgccgcgtggtggtcgcccaccgcctccccctccgcgcggatcccaatccgggcgcgccgcacgggttcgacttctccctcgacccgcacgcgctcccgctccagctctcccatggcgtcccccgccccgtcgtcttcgtcggcgtgctcccctccgccgtcgccgaggccgtccaggcgtccgacgagctcgcggccgatctcctcgcgcggttctcatgctacctggtgttcctccccgccaagctccacgccgacttctacgacggcttctgcaagcactacatgtggccgcatctccactatctcctcccgctcgcgccctcctacggcaggggcggcggcctccccttcaacggcgacctctaccgcgccttcctcaccgtcaacacccacttcgccgagcgcgtgttcgagctcctcaaccccgacgaggacctggtgttcgtccacgactaccacctctgggcgttccccaccttcctccgccacaaatccccgcgcgcccgcataggtttcttcctccactctcccttcccctcctccgagctcttccgcgccatccccgtccgcgaggacctcctccgcgccctcctcaacgccgatctcgtgggcttccacaccttcgattacgcgcgccacttcctctccgcgtgctccagggtcctcggcctctccaaccgctcgcgccgcggctacatcgggatcgagtacttcggccgcacggtggtcgtcaagatcctctccgtcggcatcgacatgggccagctccgcgcggttctgccgttgccggagacggtcgccaaggccaacgagattgctgacaagtacaggggacgacagctgatgctcggcgtggacgacatggatttgttcaaggggattgggctcaagctcttggccatggagaggctgctggagtcgcgggcggacttgcgtggccaggtggtcctcgtgcagatcaacaacccggcgcggagccttggccgcgacgtcgacgaggtccgcgcggaggtgctggcgatccgtgaccggatcaatgcccggttcggctgggcggggtacgagccggtggttgtgatcgacggcgccatgccgatgcacgacaaggtggcgttctacacgtccgcggacatctgcatcgtgaatgccgtgcgcgacgggctgaacaggataccgtacttctacaccgtgtgccggcaggagggcccggttcccaccgctcctgccgggaagccgaggcagagcgccatcatcgtgtcagagtttgtcgggtgctcgccgtcgctgagcggcgcgatccgcgtcaacccctggaacgtggacgacgtcgcggacgccatgaacacggcgctgaggatgagcgacggcgagaagcagctgcgccaggagaagcactacaggtacgtgagcacgcacgacgtcgtctactgggcgcagtcgttcgaccaggacctgcagaaggcctgcaaggacaactcgtccatggtgatcctcaacttcggcctcggcatgggcttccgcgtcgtcgcgctcggccccagcttcaagaaactctcacccgagctcattgaccaagcataccgccagactggcaacaggctcatcttactggactacgatggcacagtgatgccacaggggctgatcaacaaggcgcccagtgaggaagtgatccgtactctgaatgaactgtgctctgatccgatgaacaccgttttcgtcgtcagcgggcggggcaaggatgaactggctgaatggtttgcaccatgcgacgagaagctggggatctctgcagagcacggctacttcacaaggtggagcagggattctccctgggagtcatgcaagttggtgacacattttaattggaagaacatcgcagggcctgtaatgaagcactacagtgatgcaaccgatgggtcatacatcgaggttaaagaaacatcactagtgtggcactatgaggaagccgatccggattttggatcatgccaggccaaagagctccaggaccacctgcagaatgtgcttgcgaacgagccagtctttgtgaagagcggccatcagattgtggaagttaatcctcagggtgtgggcaaaggagtcgccgtgcgtaatctcatttcaaccatgggaaatcgtggcagcttgccagatttcatcctctgcgtcggcgatgaccggtcggatgaagacatgttcgaggctatgatcagcccttcgcctgcgttcccggagactgcacagatcttcccctgcactgttggcaacaagccgagcttggccaagtactacctggatgacccggctgatgttgtgaagatgctccagggcctgacggactcgccgacccagcagcaaccgcggccccccgtctcgttcgaaaactcgctagatgattga</cdnaseq>|
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| − | AA = <aaseq>MPSLPNSGDEGGAPPPTPPPPGARRVVVAHRLPLRADPNPGAPH GFDFSLDPHALPLQLSHGVPRPVVFVGVLPSAVAEAVQASDELAADLLARFSCYLVFL PAKLHADFYDGFCKHYMWPHLHYLLPLAPSYGRGGGLPFNGDLYRAFLTVNTHFAERV FELLNPDEDLVFVHDYHLWAFPTFLRHKSPRARIGFFLHSPFPSSELFRAIPVREDLL RALLNADLVGFHTFDYARHFLSACSRVLGLSNRSRRGYIGIEYFGRTVVVKILSVGID MGQLRAVLPLPETVAKANEIADKYRGRQLMLGVDDMDLFKGIGLKLLAMERLLESRAD LRGQVVLVQINNPARSLGRDVDEVRAEVLAIRDRINARFGWAGYEPVVVIDGAMPMHD KVAFYTSADICIVNAVRDGLNRIPYFYTVCRQEGPVPTAPAGKPRQSAIIVSEFVGCS PSLSGAIRVNPWNVDDVADAMNTALRMSDGEKQLRQEKHYRYVSTHDVVYWAQSFDQD LQKACKDNSSMVILNFGLGMGFRVVALGPSFKKLSPELIDQAYRQTGNRLILLDYDGT VMPQGLINKAPSEEVIRTLNELCSDPMNTVFVVSGRGKDELAEWFAPCDEKLGISAEH GYFTRWSRDSPWESCKLVTHFNWKNIAGPVMKHYSDATDGSYIEVKETSLVWHYEEAD PDFGSCQAKELQDHLQNVLANEPVFVKSGHQIVEVNPQGVGKGVAVRNLISTMGNRGS LPDFILCVGDDRSDEDMFEAMISPSPAFPETAQIFPCTVGNKPSLAKYYLDDPADVVK MLQGLTDSPTQQQPRPPVSFENSLDD</aaseq>|
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| − | DNA = <dnaseqindica>52..1937#2516..2789#3240..3554#gtccagttccacgaaaacccaaacggccaaacccctgtgtttgccgtgacgatgccctccctccccaactccggcgacgagggcggcgccccgcctccgactccgccgccgccgggggcgcgccgcgtggtggtcgcccaccgcctccccctccgcgcggatcccaatccgggcgcgccgcacgggttcgacttctccctcgacccgcacgcgctcccgctccagctctcccatggcgtcccccgccccgtcgtcttcgtcggcgtgctcccctccgccgtcgccgaggccgtccaggcgtccgacgagctcgcggccgatctcctcgcgcggttctcatgctacctggtgttcctccccgccaagctccacgccgacttctacgacggcttctgcaagcactacatgtggccgcatctccactatctcctcccgctcgcgccctcctacggcaggggcggcggcctccccttcaacggcgacctctaccgcgccttcctcaccgtcaacacccacttcgccgagcgcgtgttcgagctcctcaaccccgacgaggacctggtgttcgtccacgactaccacctctgggcgttccccaccttcctccgccacaaatccccgcgcgcccgcataggtttcttcctccactctcccttcccctcctccgagctcttccgcgccatccccgtccgcgaggacctcctccgcgccctcctcaacgccgatctcgtgggcttccacaccttcgattacgcgcgccacttcctctccgcgtgctccagggtcctcggcctctccaaccgctcgcgccgcggctacatcgggatcgagtacttcggccgcacggtggtcgtcaagatcctctccgtcggcatcgacatgggccagctccgcgcggttctgccgttgccggagacggtcgccaaggccaacgagattgctgacaagtacaggggacgacagctgatgctcggcgtggacgacatggatttgttcaaggggattgggctcaagctcttggccatggagaggctgctggagtcgcgggcggacttgcgtggccaggtggtcctcgtgcagatcaacaacccggcgcggagccttggccgcgacgtcgacgaggtccgcgcggaggtgctggcgatccgtgaccggatcaatgcccggttcggctgggcggggtacgagccggtggttgtgatcgacggcgccatgccgatgcacgacaaggtggcgttctacacgtccgcggacatctgcatcgtgaatgccgtgcgcgacgggctgaacaggataccgtacttctacaccgtgtgccggcaggagggcccggttcccaccgctcctgccgggaagccgaggcagagcgccatcatcgtgtcagagtttgtcgggtgctcgccgtcgctgagcggcgcgatccgcgtcaacccctggaacgtggacgacgtcgcggacgccatgaacacggcgctgaggatgagcgacggcgagaagcagctgcgccaggagaagcactacaggtacgtgagcacgcacgacgtcgtctactgggcgcagtcgttcgaccaggacctgcagaaggcctgcaaggacaactcgtccatggtgatcctcaacttcggcctcggcatgggcttccgcgtcgtcgcgctcggccccagcttcaagaaactctcacccgagctcattgaccaagcataccgccagactggcaacaggctcatcttactggactacgatggcacagtgatgccacaggggctgatcaacaaggcgcccagtgaggaagtgatccgtactctgaatgaactgtgctctgatccgatgaacaccgttttcgtcgtcagcgggcggggcaaggatgaactggctgaatggtttgcaccatgcgacgagaagctggggatctctgcagagcacggctacttcacaaggtaagcacaagatttcctccctattctcttggcatgttccttcgttggttaggtgaaactgtggttgctgtcggcctaggcattgttaggtgacaaatggataaggtgcctggtatagtgtatattctcgaactagcaattcttctgtaatcaggtcactaccctgaactgggtggtgacaatctgctggcttgcattatgatactaaacaggagtagagatggtggtatttaagtagagggcctttgtgattctatcttttctgttagaatacacatgttctgaatgaaatactacatgtgaacatgacaaagaggatttgctttatctagaataccgaaaaggttggtaatgagtcgatgttgattgacaaatgctctgtaaagtatagctattctgaattttatataggttcttcacagtatagataatacagggcaacaactgactactcctgtgggattgaagttcttgattctactagcattactttatgttggtgtgcatgtgactgtgaggagattaaggaataagattggctgcttgtgcttaagtagtaactctgttttgtttatcaggtggagcagggattctccctgggagtcatgcaagttggtgacacattttaattggaagaacatcgcagggcctgtaatgaagcactacagtgatgcaaccgatgggtcatacatcgaggttaaagaaacatcactagtgtggcactatgaggaagccgatccggattttggatcatgccaggccaaagagctccaggaccacctgcagaatgtgcttgcgaacgagccagtctttgtgaagagcggccatcagattgtggaagttaatcctcaggtacggaaacctatattaaagtcgccccgtatcatctctttggtagtatttctgaatgggttcacatccgatatatctctgtatcatctttttggattgctatctcactttcgaatgctgagaccaagacgaaaagaacaagtctcaattccaccggtagctgtcatgatcaagcaagcattgctttcagaccaaaagacattagattagactgactttaagaagcttcatccacctcatattcagctaccatatccctttggcacgtactgcatcattcaatataattcggtctaataaaaatttcatgggccacgcctgctacatatcttctgtcggctgtattttttttcctaatatctccagcttatctcctcgagcagtctttttttttatttgccatcacttgtcctattttgtctcaccacctatactattattcctatgcagggtgtgggcaaaggagtcgccgtgcgtaatctcatttcaaccatgggaaatcgtggcagcttgccagatttcatcctctgcgtcggcgatgaccggtcggatgaagacatgttcgaggctatgatcagcccttcgcctgcgttcccggagactgcacagatcttcccctgcactgttggcaacaagccgagcttggccaagtactacctggatgacccggctgatgttgtgaagatgctccagggcctgacggactcgccgacccagcagcaaccgcggccccccgtctcgttcgaaaactcgctagatgattgaaggatcaagaagaggtcgcgtgacatatatgctcgtgagaaagagccccgttcatttcgttcatgattgctttttgtaattagcagaaagagagagagaaaaaatggttttggggggaggtgtgaaaatgggaaaagggcaaaggtgagcaaaaactgactagtgatcgatcggtaggttacagatgttcatgttttacccggtaggcagttcttttgtacaaacgtcaaaagttttttttttccctgcctagatcgtcagaagaaacaaaatcatatggttcggtg</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001068460.1 RefSeq:Os08g0445700]|
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| − | }}
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| | [[Category:Genes]] | | [[Category:Genes]] |
| | [[Category:Japonica mRNA]] | | [[Category:Japonica mRNA]] |
