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| − | {{JaponicaGene|
| + | The rice '''''Os08g0445700''''' was reported as '''''OsTPS1''''' in 2011 <ref name="ref1" /> by researchers from China. |
| − | GeneName = Os08g0445700|
| + | |
| − | Description = HAD-superfamily hydrolase subfamily IIB protein|
| + | ==Annotated Information== |
| − | Version = NM_001068460.1 GI:115476657 GeneID:4345706|
| + | ===Function=== |
| − | Length = 3841 bp|
| + | * Trehalose-6-phosphate synthase (TPS) is a key enzyme for trehalose biosynthesis. |
| − | Definition = Oryza sativa Japonica Group Os08g0445700, complete gene.|
| + | * Overexpression of the trehalose-6-phosphate synthase gene '''''OsTPS1''''' enhances abiotic stress tolerance in rice |
| − | Source = Oryza sativa Japonica Group
| + | * Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms. |
| | + | * In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). The genome of rice (Oryza sativa) contains 11 OsTPS genes, and only OsTPS1 shows TPS activity. |
| | + | |
| | + | ===Phenotypic analysis=== |
| | + | * Leaves of OsTPS1 knockout (KO) plants were more sensitive to drought or cold stress than were the wild types. |
| | + | * Furthermore, transgenic rice of UBI::CBF1 had high expression OsTPS1 mRNA, suggesting that '''''OsTPS1''''' is regulated by the CBF/DREB transcription factor. |
| | + | * '''''OsTPS1''''' overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant phenotypic changes |
| | + | |
| | + | ===Expression=== |
| | + | * Reverse transcriptase-PCR analysis of OsTPS1 showed that OsTPS1 is inducible by drought, salt, cold, and ABA. |
| | + | |
| | + | You can also add sub-section(s) at will. |
| | + | |
| | + | ==Labs working on this gene== |
| | + | * Key Laboratory of Gene Engineering Drug and Biotechnology, College of Life Sciences, Beijing Normal University, Beijing, 100875, People's Republic of China. |
| | + | |
| | + | ==References== |
| | + | <references> |
| | + | * <ref name="ref1"> |
| | + | Li HW, Zang BS, Deng XW, Wang XP. Overexpression of the trehalose-6-phosphate |
| | + | synthase gene OsTPS1 enhances abiotic stress tolerance in rice. Planta. 2011 |
| | + | Nov;234(5):1007-18. doi: 10.1007/s00425-011-1458-0. PubMed PMID: 21698458. |
| | + | </ref> |
| | + | </references> |
| | + | |
| | + | ==Structured Information== |
| | | | |
| − | ORGANISM Oryza sativa Japonica Group
| |
| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
| |
| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
| |
| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 8|Chromosome 8]]|
| |
| − | AP = Chromosome 8:21810273..21814113|
| |
| − | CDS = 21810324..21812209,21812788..21813061,21813512..21813826|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008401:21810273..21814113
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| − | source=RiceChromosome08
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008401:21810273..21814113
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| − | source=RiceChromosome08
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| − | preset=GeneLocation
| |
| − | </gbrowseImage2>|
| |
| − | CDNA = <cdnaseq>atgccctccctccccaactccggcgacgagggcggcgccccgcctccgactccgccgccgccgggggcgcgccgcgtggtggtcgcccaccgcctccccctccgcgcggatcccaatccgggcgcgccgcacgggttcgacttctccctcgacccgcacgcgctcccgctccagctctcccatggcgtcccccgccccgtcgtcttcgtcggcgtgctcccctccgccgtcgccgaggccgtccaggcgtccgacgagctcgcggccgatctcctcgcgcggttctcatgctacctggtgttcctccccgccaagctccacgccgacttctacgacggcttctgcaagcactacatgtggccgcatctccactatctcctcccgctcgcgccctcctacggcaggggcggcggcctccccttcaacggcgacctctaccgcgccttcctcaccgtcaacacccacttcgccgagcgcgtgttcgagctcctcaaccccgacgaggacctggtgttcgtccacgactaccacctctgggcgttccccaccttcctccgccacaaatccccgcgcgcccgcataggtttcttcctccactctcccttcccctcctccgagctcttccgcgccatccccgtccgcgaggacctcctccgcgccctcctcaacgccgatctcgtgggcttccacaccttcgattacgcgcgccacttcctctccgcgtgctccagggtcctcggcctctccaaccgctcgcgccgcggctacatcgggatcgagtacttcggccgcacggtggtcgtcaagatcctctccgtcggcatcgacatgggccagctccgcgcggttctgccgttgccggagacggtcgccaaggccaacgagattgctgacaagtacaggggacgacagctgatgctcggcgtggacgacatggatttgttcaaggggattgggctcaagctcttggccatggagaggctgctggagtcgcgggcggacttgcgtggccaggtggtcctcgtgcagatcaacaacccggcgcggagccttggccgcgacgtcgacgaggtccgcgcggaggtgctggcgatccgtgaccggatcaatgcccggttcggctgggcggggtacgagccggtggttgtgatcgacggcgccatgccgatgcacgacaaggtggcgttctacacgtccgcggacatctgcatcgtgaatgccgtgcgcgacgggctgaacaggataccgtacttctacaccgtgtgccggcaggagggcccggttcccaccgctcctgccgggaagccgaggcagagcgccatcatcgtgtcagagtttgtcgggtgctcgccgtcgctgagcggcgcgatccgcgtcaacccctggaacgtggacgacgtcgcggacgccatgaacacggcgctgaggatgagcgacggcgagaagcagctgcgccaggagaagcactacaggtacgtgagcacgcacgacgtcgtctactgggcgcagtcgttcgaccaggacctgcagaaggcctgcaaggacaactcgtccatggtgatcctcaacttcggcctcggcatgggcttccgcgtcgtcgcgctcggccccagcttcaagaaactctcacccgagctcattgaccaagcataccgccagactggcaacaggctcatcttactggactacgatggcacagtgatgccacaggggctgatcaacaaggcgcccagtgaggaagtgatccgtactctgaatgaactgtgctctgatccgatgaacaccgttttcgtcgtcagcgggcggggcaaggatgaactggctgaatggtttgcaccatgcgacgagaagctggggatctctgcagagcacggctacttcacaaggtggagcagggattctccctgggagtcatgcaagttggtgacacattttaattggaagaacatcgcagggcctgtaatgaagcactacagtgatgcaaccgatgggtcatacatcgaggttaaagaaacatcactagtgtggcactatgaggaagccgatccggattttggatcatgccaggccaaagagctccaggaccacctgcagaatgtgcttgcgaacgagccagtctttgtgaagagcggccatcagattgtggaagttaatcctcagggtgtgggcaaaggagtcgccgtgcgtaatctcatttcaaccatgggaaatcgtggcagcttgccagatttcatcctctgcgtcggcgatgaccggtcggatgaagacatgttcgaggctatgatcagcccttcgcctgcgttcccggagactgcacagatcttcccctgcactgttggcaacaagccgagcttggccaagtactacctggatgacccggctgatgttgtgaagatgctccagggcctgacggactcgccgacccagcagcaaccgcggccccccgtctcgttcgaaaactcgctagatgattga</cdnaseq>|
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| − | AA = <aaseq>MPSLPNSGDEGGAPPPTPPPPGARRVVVAHRLPLRADPNPGAPH GFDFSLDPHALPLQLSHGVPRPVVFVGVLPSAVAEAVQASDELAADLLARFSCYLVFL PAKLHADFYDGFCKHYMWPHLHYLLPLAPSYGRGGGLPFNGDLYRAFLTVNTHFAERV FELLNPDEDLVFVHDYHLWAFPTFLRHKSPRARIGFFLHSPFPSSELFRAIPVREDLL RALLNADLVGFHTFDYARHFLSACSRVLGLSNRSRRGYIGIEYFGRTVVVKILSVGID MGQLRAVLPLPETVAKANEIADKYRGRQLMLGVDDMDLFKGIGLKLLAMERLLESRAD LRGQVVLVQINNPARSLGRDVDEVRAEVLAIRDRINARFGWAGYEPVVVIDGAMPMHD KVAFYTSADICIVNAVRDGLNRIPYFYTVCRQEGPVPTAPAGKPRQSAIIVSEFVGCS PSLSGAIRVNPWNVDDVADAMNTALRMSDGEKQLRQEKHYRYVSTHDVVYWAQSFDQD LQKACKDNSSMVILNFGLGMGFRVVALGPSFKKLSPELIDQAYRQTGNRLILLDYDGT VMPQGLINKAPSEEVIRTLNELCSDPMNTVFVVSGRGKDELAEWFAPCDEKLGISAEH GYFTRWSRDSPWESCKLVTHFNWKNIAGPVMKHYSDATDGSYIEVKETSLVWHYEEAD PDFGSCQAKELQDHLQNVLANEPVFVKSGHQIVEVNPQGVGKGVAVRNLISTMGNRGS LPDFILCVGDDRSDEDMFEAMISPSPAFPETAQIFPCTVGNKPSLAKYYLDDPADVVK MLQGLTDSPTQQQPRPPVSFENSLDD</aaseq>|
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| − | DNA = <dnaseqindica>52..1937#2516..2789#3240..3554#gtccagttccacgaaaacccaaacggccaaacccctgtgtttgccgtgacgatgccctccctccccaactccggcgacgagggcggcgccccgcctccgactccgccgccgccgggggcgcgccgcgtggtggtcgcccaccgcctccccctccgcgcggatcccaatccgggcgcgccgcacgggttcgacttctccctcgacccgcacgcgctcccgctccagctctcccatggcgtcccccgccccgtcgtcttcgtcggcgtgctcccctccgccgtcgccgaggccgtccaggcgtccgacgagctcgcggccgatctcctcgcgcggttctcatgctacctggtgttcctccccgccaagctccacgccgacttctacgacggcttctgcaagcactacatgtggccgcatctccactatctcctcccgctcgcgccctcctacggcaggggcggcggcctccccttcaacggcgacctctaccgcgccttcctcaccgtcaacacccacttcgccgagcgcgtgttcgagctcctcaaccccgacgaggacctggtgttcgtccacgactaccacctctgggcgttccccaccttcctccgccacaaatccccgcgcgcccgcataggtttcttcctccactctcccttcccctcctccgagctcttccgcgccatccccgtccgcgaggacctcctccgcgccctcctcaacgccgatctcgtgggcttccacaccttcgattacgcgcgccacttcctctccgcgtgctccagggtcctcggcctctccaaccgctcgcgccgcggctacatcgggatcgagtacttcggccgcacggtggtcgtcaagatcctctccgtcggcatcgacatgggccagctccgcgcggttctgccgttgccggagacggtcgccaaggccaacgagattgctgacaagtacaggggacgacagctgatgctcggcgtggacgacatggatttgttcaaggggattgggctcaagctcttggccatggagaggctgctggagtcgcgggcggacttgcgtggccaggtggtcctcgtgcagatcaacaacccggcgcggagccttggccgcgacgtcgacgaggtccgcgcggaggtgctggcgatccgtgaccggatcaatgcccggttcggctgggcggggtacgagccggtggttgtgatcgacggcgccatgccgatgcacgacaaggtggcgttctacacgtccgcggacatctgcatcgtgaatgccgtgcgcgacgggctgaacaggataccgtacttctacaccgtgtgccggcaggagggcccggttcccaccgctcctgccgggaagccgaggcagagcgccatcatcgtgtcagagtttgtcgggtgctcgccgtcgctgagcggcgcgatccgcgtcaacccctggaacgtggacgacgtcgcggacgccatgaacacggcgctgaggatgagcgacggcgagaagcagctgcgccaggagaagcactacaggtacgtgagcacgcacgacgtcgtctactgggcgcagtcgttcgaccaggacctgcagaaggcctgcaaggacaactcgtccatggtgatcctcaacttcggcctcggcatgggcttccgcgtcgtcgcgctcggccccagcttcaagaaactctcacccgagctcattgaccaagcataccgccagactggcaacaggctcatcttactggactacgatggcacagtgatgccacaggggctgatcaacaaggcgcccagtgaggaagtgatccgtactctgaatgaactgtgctctgatccgatgaacaccgttttcgtcgtcagcgggcggggcaaggatgaactggctgaatggtttgcaccatgcgacgagaagctggggatctctgcagagcacggctacttcacaaggtaagcacaagatttcctccctattctcttggcatgttccttcgttggttaggtgaaactgtggttgctgtcggcctaggcattgttaggtgacaaatggataaggtgcctggtatagtgtatattctcgaactagcaattcttctgtaatcaggtcactaccctgaactgggtggtgacaatctgctggcttgcattatgatactaaacaggagtagagatggtggtatttaagtagagggcctttgtgattctatcttttctgttagaatacacatgttctgaatgaaatactacatgtgaacatgacaaagaggatttgctttatctagaataccgaaaaggttggtaatgagtcgatgttgattgacaaatgctctgtaaagtatagctattctgaattttatataggttcttcacagtatagataatacagggcaacaactgactactcctgtgggattgaagttcttgattctactagcattactttatgttggtgtgcatgtgactgtgaggagattaaggaataagattggctgcttgtgcttaagtagtaactctgttttgtttatcaggtggagcagggattctccctgggagtcatgcaagttggtgacacattttaattggaagaacatcgcagggcctgtaatgaagcactacagtgatgcaaccgatgggtcatacatcgaggttaaagaaacatcactagtgtggcactatgaggaagccgatccggattttggatcatgccaggccaaagagctccaggaccacctgcagaatgtgcttgcgaacgagccagtctttgtgaagagcggccatcagattgtggaagttaatcctcaggtacggaaacctatattaaagtcgccccgtatcatctctttggtagtatttctgaatgggttcacatccgatatatctctgtatcatctttttggattgctatctcactttcgaatgctgagaccaagacgaaaagaacaagtctcaattccaccggtagctgtcatgatcaagcaagcattgctttcagaccaaaagacattagattagactgactttaagaagcttcatccacctcatattcagctaccatatccctttggcacgtactgcatcattcaatataattcggtctaataaaaatttcatgggccacgcctgctacatatcttctgtcggctgtattttttttcctaatatctccagcttatctcctcgagcagtctttttttttatttgccatcacttgtcctattttgtctcaccacctatactattattcctatgcagggtgtgggcaaaggagtcgccgtgcgtaatctcatttcaaccatgggaaatcgtggcagcttgccagatttcatcctctgcgtcggcgatgaccggtcggatgaagacatgttcgaggctatgatcagcccttcgcctgcgttcccggagactgcacagatcttcccctgcactgttggcaacaagccgagcttggccaagtactacctggatgacccggctgatgttgtgaagatgctccagggcctgacggactcgccgacccagcagcaaccgcggccccccgtctcgttcgaaaactcgctagatgattgaaggatcaagaagaggtcgcgtgacatatatgctcgtgagaaagagccccgttcatttcgttcatgattgctttttgtaattagcagaaagagagagagaaaaaatggttttggggggaggtgtgaaaatgggaaaagggcaaaggtgagcaaaaactgactagtgatcgatcggtaggttacagatgttcatgttttacccggtaggcagttcttttgtacaaacgtcaaaagttttttttttccctgcctagatcgtcagaagaaacaaaatcatatggttcggtg</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001068460.1 RefSeq:Os08g0445700]|
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| − | }}
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| | [[Category:Genes]] | | [[Category:Genes]] |
| | [[Category:Japonica mRNA]] | | [[Category:Japonica mRNA]] |
