Difference between revisions of "Os05g0341600"
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| − | the rice gene '''Os05g0341600''' was recongized as ''' | + | the rice gene '''Os05g0341600''' was recongized as '''''RAB21''''' in 1988, which is induced when plants are subject to water-stress. |
==Annotated Information== | ==Annotated Information== | ||
| + | ===Gene Symbol=== | ||
| + | *'''''Os05g0341600''''' '''<=>''' '''''OsRab5D1,Rab5D1,RAB21''''' | ||
| + | [[File:Abscisic acid.png|right|thumb|200px|]] | ||
| + | |||
===Function=== | ===Function=== | ||
| − | the rice gene '''Os05g0341600''' was recongized as ''' | + | * the rice gene '''Os05g0341600''' was recongized as '''''RAB21''''' in 1988, which is induced to balance water-stress of plants. encoding a basic, glycine-rich protein (mol.wt 16 529) which has a duplicated domain structure. |
| + | * Immunoblots probed with antibodies raised against Bgalactosidase/RAB 21 fusion protein detect RAB 21 protein only in cytosolic cell fractions. <ref name="ref1" /><ref name="ref1" /><ref name="ref2" /><ref name="ref3" /> | ||
===Expression=== | ===Expression=== | ||
| − | RAB 21 mRNA and protein accumulate in rice embryos, leaves, roots and callus-derived suspension cells upon treatment with NaCl (200 mM) and/or the plant hormone abscisic acid (10 /tMABA). The effects of NaCI and ABA are not cumulative, suggesting that these two inducers share a common response pathway. Induction of RAB 21 mRNA accumulation by ABA is rapid (< 15 min in suspension cells) and does not require protein synthesis, indicating that preformed nuclear and/or cytosolic factors mediate the response to this hormone. | + | * RAB 21 mRNA and protein accumulate in rice embryos, leaves, roots and callus-derived suspension cells upon treatment with NaCl (200 mM) and/or the plant hormone abscisic acid (10 /tMABA). |
| + | * The effects of NaCI and ABA are not cumulative, suggesting that these two inducers share a common response pathway. Induction of RAB 21 mRNA accumulation by ABA is rapid (< 15 min in suspension cells) and does not require protein synthesis, indicating that preformed nuclear and/or cytosolic factors mediate the response to this hormone. | ||
| + | * the reseachers have characterized the RAB 21 gene by deternmining the complete nucleotide sequence of a nearly full-length cDNA and corresponding genomiccopy, and by mapping the start site of its major transcript. The proximal promoter region contains various GC-rich repeats. | ||
| − | === | + | ===Knowledge Extension=== |
| − | + | * The hormone abscisic acid (ABA) mediates a number of important physiological processes in plants (King, 1976; Jones et al., 1987). | |
| − | + | * Developmental studies have shown that ABA induces the accumulation of specific mRNAs and proteins late during embryogenesis in seeds of diverse species (Galau et al., 1986; Finklestein et al., 1985; Litts et al.,1987). | |
| − | + | * At this time, the level of endogenous ABA increases,the seeds desiccate and the embryos of some species become dormant (King, 1976; Suzuki et al., 1981). | |
| + | * In cereal seeds,some of the 'late' developmental mRNAs are long-lived in mature, dry grains, but are rapidly degraded during seed germination. | ||
| + | * However, exogenously applied ABA causes the precocious accumulation of these mRNAs in immature embryos and their reappearance in germinating seeds (Finklestein et al., 1985; Mundy et al., 1986). | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | Laboratory of Plant Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA | + | * Laboratory of Plant Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA |
==References== | ==References== | ||
<references> | <references> | ||
<ref name="ref1">Mundy, J. and N.-H. Chua (1988). "Abscisic acid and water-stress induce the expression of a novel rice gene." The EMBO Journal 7(8): 2279.</ref> | <ref name="ref1">Mundy, J. and N.-H. Chua (1988). "Abscisic acid and water-stress induce the expression of a novel rice gene." The EMBO Journal 7(8): 2279.</ref> | ||
| + | <ref name="ref2">Bray EA, Beachy RN. Regulation by ABA of beta-Conglycinin Expression in Cultured Developing Soybean Cotyledons. Plant Physiol. 1985 Nov;79(3):746–750.</ref> | ||
| + | <ref name="ref3">Finkelstein RR, Tenbarge KM, Shumway JE, Crouch ML. Role of ABA in Maturation of Rapeseed Embryos. Plant Physiol. 1985 Jul;78(3):630–636.</ref> | ||
</references> | </references> | ||
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==Structured Information== | ==Structured Information== | ||
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[[Category:Genes]] | [[Category:Genes]] | ||
[[Category:Japonica mRNA]] | [[Category:Japonica mRNA]] | ||
Latest revision as of 15:19, 6 May 2017
the rice gene Os05g0341600 was recongized as RAB21 in 1988, which is induced when plants are subject to water-stress.
Contents
Annotated Information
Gene Symbol
- Os05g0341600 <=> OsRab5D1,Rab5D1,RAB21
Function
- the rice gene Os05g0341600 was recongized as RAB21 in 1988, which is induced to balance water-stress of plants. encoding a basic, glycine-rich protein (mol.wt 16 529) which has a duplicated domain structure.
- Immunoblots probed with antibodies raised against Bgalactosidase/RAB 21 fusion protein detect RAB 21 protein only in cytosolic cell fractions. [1][1][2][3]
Expression
- RAB 21 mRNA and protein accumulate in rice embryos, leaves, roots and callus-derived suspension cells upon treatment with NaCl (200 mM) and/or the plant hormone abscisic acid (10 /tMABA).
- The effects of NaCI and ABA are not cumulative, suggesting that these two inducers share a common response pathway. Induction of RAB 21 mRNA accumulation by ABA is rapid (< 15 min in suspension cells) and does not require protein synthesis, indicating that preformed nuclear and/or cytosolic factors mediate the response to this hormone.
- the reseachers have characterized the RAB 21 gene by deternmining the complete nucleotide sequence of a nearly full-length cDNA and corresponding genomiccopy, and by mapping the start site of its major transcript. The proximal promoter region contains various GC-rich repeats.
Knowledge Extension
- The hormone abscisic acid (ABA) mediates a number of important physiological processes in plants (King, 1976; Jones et al., 1987).
- Developmental studies have shown that ABA induces the accumulation of specific mRNAs and proteins late during embryogenesis in seeds of diverse species (Galau et al., 1986; Finklestein et al., 1985; Litts et al.,1987).
- At this time, the level of endogenous ABA increases,the seeds desiccate and the embryos of some species become dormant (King, 1976; Suzuki et al., 1981).
- In cereal seeds,some of the 'late' developmental mRNAs are long-lived in mature, dry grains, but are rapidly degraded during seed germination.
- However, exogenously applied ABA causes the precocious accumulation of these mRNAs in immature embryos and their reappearance in germinating seeds (Finklestein et al., 1985; Mundy et al., 1986).
Labs working on this gene
- Laboratory of Plant Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
References
- ↑ 1.0 1.1 Mundy, J. and N.-H. Chua (1988). "Abscisic acid and water-stress induce the expression of a novel rice gene." The EMBO Journal 7(8): 2279.
- ↑ Bray EA, Beachy RN. Regulation by ABA of beta-Conglycinin Expression in Cultured Developing Soybean Cotyledons. Plant Physiol. 1985 Nov;79(3):746–750.
- ↑ Finkelstein RR, Tenbarge KM, Shumway JE, Crouch ML. Role of ABA in Maturation of Rapeseed Embryos. Plant Physiol. 1985 Jul;78(3):630–636.
