Difference between revisions of "Os04g0607600"
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| − | + | The rice '''''Os04g0607600''''' was reported as '''''HKT1;4''''' in 2016 <ref name="ref1" /> by researchers from Japan, Italy and USA. | |
==Annotated Information== | ==Annotated Information== | ||
| + | ===Gene Symbol=== | ||
| + | *'''''Os04g0459000''''' '''''<=>''''' '''''OsHKT7''''','''''HKT7''''' | ||
===Function=== | ===Function=== | ||
| − | + | * OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress. | |
===Expression=== | ===Expression=== | ||
| − | + | * Heterologous expression analysis was performed using a salt hypersensitive mutant of S. cerevisiae (strain G19). | |
| + | * OsHKT1;4 expression increases Na+ hypersensitivity of yeast cells promoting Na+ over-accumulation. The OsHKT1;4 cDNA from rice cultivar Nipponbare was constitutively expressed in strain G19 of S. cerevisiae under the control of the GAP promoter. | ||
| + | * G19 cells harboring the empty vector or expressing OsHKT1;4 were grown on the arginine phosphate (AP) medium containing 1 mM KCl with or without the addition of 50 mM NaCl. | ||
| − | === | + | ===Phenotypic analysis=== |
| − | + | * phenotypic analysis of OsHKT1;4 RNAi plants in the vegetative growth stage revealed no profound influence on the growth and ion accumulation in comparison with WT plants upon salinity stress. | |
| + | |||
| + | ===Subcellular localization=== | ||
| + | * To determine the localization of the OsHKT1;4 protein in plant cells, we fused EGFP at the N-terminus end of OsHKT1;4 (EGFP-OsHKT1;4 was shown to be functional in X. laevis oocytes) and placed under the control of the CaMV35S promoter.The result strongly indicated that EGFP-OsHKT1;4 localizes to the PM of rice protoplasts. However, by repeating the transformation experiments several times, we often observed that EGFP-OsHKT1;4 was also present inside the cells and clustered in punctate-like structures. | ||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China; | |
| − | + | * Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland | |
| + | * State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China. | ||
| + | * State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China | ||
| + | * State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China. | ||
==References== | ==References== | ||
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Suzuki K, Yamaji N, Costa A, Okuma E, Kobayashi NI, Kashiwagi T, Katsuhara M, | ||
| + | Wang C, Tanoi K, Murata Y, Schroeder JI, Ma JF, Horie T. OsHKT1;4-mediated Na(+) | ||
| + | transport in stems contributes to Na(+) exclusion from leaf blades of rice at the | ||
| + | reproductive growth stage upon salt stress. BMC Plant Biol. 2016 Jan 19;16:22. | ||
| + | doi: 10.1186/s12870-016-0709-4. PubMed PMID: 26786707; PubMed Central PMCID: | ||
| + | PMC4719677. | ||
| + | </ref> | ||
| + | </references> | ||
==Structured Information== | ==Structured Information== | ||
| − | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 4]] | |
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| − | [[Category:Genes]] | ||
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Latest revision as of 03:12, 19 January 2018
The rice Os04g0607600 was reported as HKT1;4 in 2016 [1] by researchers from Japan, Italy and USA.
Contents
Annotated Information
Gene Symbol
- Os04g0459000 <=> OsHKT7,HKT7
Function
- OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress.
Expression
- Heterologous expression analysis was performed using a salt hypersensitive mutant of S. cerevisiae (strain G19).
- OsHKT1;4 expression increases Na+ hypersensitivity of yeast cells promoting Na+ over-accumulation. The OsHKT1;4 cDNA from rice cultivar Nipponbare was constitutively expressed in strain G19 of S. cerevisiae under the control of the GAP promoter.
- G19 cells harboring the empty vector or expressing OsHKT1;4 were grown on the arginine phosphate (AP) medium containing 1 mM KCl with or without the addition of 50 mM NaCl.
Phenotypic analysis
- phenotypic analysis of OsHKT1;4 RNAi plants in the vegetative growth stage revealed no profound influence on the growth and ion accumulation in comparison with WT plants upon salinity stress.
Subcellular localization
- To determine the localization of the OsHKT1;4 protein in plant cells, we fused EGFP at the N-terminus end of OsHKT1;4 (EGFP-OsHKT1;4 was shown to be functional in X. laevis oocytes) and placed under the control of the CaMV35S promoter.The result strongly indicated that EGFP-OsHKT1;4 localizes to the PM of rice protoplasts. However, by repeating the transformation experiments several times, we often observed that EGFP-OsHKT1;4 was also present inside the cells and clustered in punctate-like structures.
You can also add sub-section(s) at will.
Labs working on this gene
- State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
- Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
- State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
- State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
- State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
References
- ↑ Suzuki K, Yamaji N, Costa A, Okuma E, Kobayashi NI, Kashiwagi T, Katsuhara M, Wang C, Tanoi K, Murata Y, Schroeder JI, Ma JF, Horie T. OsHKT1;4-mediated Na(+) transport in stems contributes to Na(+) exclusion from leaf blades of rice at the reproductive growth stage upon salt stress. BMC Plant Biol. 2016 Jan 19;16:22. doi: 10.1186/s12870-016-0709-4. PubMed PMID: 26786707; PubMed Central PMCID: PMC4719677.
