Difference between revisions of "Os06g0625400"
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| − | + | The rice gene Os06g0625400 was reported as '''''spp''''' in 2010<ref name="ref1" /> by researchers from China. | |
==Annotated Information== | ==Annotated Information== | ||
| + | ===Gene Symbol=== | ||
| + | *'''''Os06g0625400 <=> spp, Osspp | ||
| + | |||
===Function=== | ===Function=== | ||
| − | + | * '''''SPP''''' encodes a metalloendopeptidase that cleaves a broad range of precursor substrates. | |
| − | + | * '''''SPP''''' is essential for chloroplast biogenesis at the early growth stage and for rice root development | |
| − | ===Expression=== | + | ===Mutation=== |
| − | + | * '''''spp''''' mutant shows leaf chlorosis at the early seedling stage but inhibition of root growth during the whole growth period. | |
| + | * The expression of some genes associated with chloroplast development was decreased in young seedlings of the spp mutant, but not in 14-day-old seedlings. Western blot analysis revealed that the Rubisco small subunit is not precisely processed in the spp mutant in 7-day-old seedlings, but the processing activity in the spp mutant is restored in 14-day-old seedlings. | ||
| + | * Moreover, the expression levels of Cab1R and Cab2R for the light-harvesting chlorophyll a / b -binding protein (LHCP) were highly up-regulated in the transgenic plants with overexpression of SPP. | ||
| + | ===Expression === | ||
| + | * Semi-quantitative RT–PCR analysis was performed to investigate the expression pattern of SPP in different tissues. The results showed that SPP was expressed in all the tested tissues, with a similar level in the spp mutant and the WT, and with higher expression levels in chloroplast-containing green tissues. SPP mRNAs also accumulated in roots. No signifi cant change in transcript levels was observed in the WT and the spp mutant shoots of 7- and 14-day-old plants. Furthermore, no differences in SPP mRNAs were observed when the WT and spp mutant plants were grown under light and dark conditions. | ||
| + | ===Sublocalization=== | ||
| + | * Subcellular localization analysis indicates that SPP is targeted to the chloroplast. | ||
===Evolution=== | ===Evolution=== | ||
| − | |||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
| + | ==Labs working on this gene== | ||
| + | * State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, PR China | ||
| + | ==References== | ||
| + | <references> | ||
| + | * <ref name="ref1"> | ||
| + | Yue R, Wang X, Chen J, Ma X, Zhang H, Mao C, Wu P. A rice stromal processing | ||
| + | peptidase regulates chloroplast and root development. Plant Cell Physiol. 2010 | ||
| + | Mar;51(3):475-85. doi: 10.1093/pcp/pcq012. PubMed PMID: 20097911. | ||
| − | + | </ref> | |
| − | + | </references> | |
| − | |||
| − | |||
==Structured Information== | ==Structured Information== | ||
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 6]] | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 6]] | ||
Latest revision as of 12:16, 10 December 2016
The rice gene Os06g0625400 was reported as spp in 2010[1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os06g0625400 <=> spp, Osspp
Function
- SPP encodes a metalloendopeptidase that cleaves a broad range of precursor substrates.
- SPP is essential for chloroplast biogenesis at the early growth stage and for rice root development
Mutation
- spp mutant shows leaf chlorosis at the early seedling stage but inhibition of root growth during the whole growth period.
- The expression of some genes associated with chloroplast development was decreased in young seedlings of the spp mutant, but not in 14-day-old seedlings. Western blot analysis revealed that the Rubisco small subunit is not precisely processed in the spp mutant in 7-day-old seedlings, but the processing activity in the spp mutant is restored in 14-day-old seedlings.
- Moreover, the expression levels of Cab1R and Cab2R for the light-harvesting chlorophyll a / b -binding protein (LHCP) were highly up-regulated in the transgenic plants with overexpression of SPP.
Expression
- Semi-quantitative RT–PCR analysis was performed to investigate the expression pattern of SPP in different tissues. The results showed that SPP was expressed in all the tested tissues, with a similar level in the spp mutant and the WT, and with higher expression levels in chloroplast-containing green tissues. SPP mRNAs also accumulated in roots. No signifi cant change in transcript levels was observed in the WT and the spp mutant shoots of 7- and 14-day-old plants. Furthermore, no differences in SPP mRNAs were observed when the WT and spp mutant plants were grown under light and dark conditions.
Sublocalization
- Subcellular localization analysis indicates that SPP is targeted to the chloroplast.
Evolution
You can also add sub-section(s) at will.
Labs working on this gene
- State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, PR China
References
- ↑ Yue R, Wang X, Chen J, Ma X, Zhang H, Mao C, Wu P. A rice stromal processing peptidase regulates chloroplast and root development. Plant Cell Physiol. 2010 Mar;51(3):475-85. doi: 10.1093/pcp/pcq012. PubMed PMID: 20097911.
