Difference between revisions of "Os09g0563300"
(→Annotated Information) |
|||
| (7 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | + | The rice '''''Os09g0563300''''' was reported as '''''TCD9''''' in 2014 <ref name="ref1" /> by researchers from China. | |
==Annotated Information== | ==Annotated Information== | ||
| + | [[File:58-Os09g0563300.png|right|thumb|427px|'''Figure 6.''' ''Subcellular localization of pMON530-SPTCD9-GFP protein.<ref name="ref1" />.'']] | ||
| + | ===Gene Symbol=== | ||
| + | *'''''Os09g0563300''''' '''<=>''' '''''OsTCD9,TCD9''''' | ||
===Function=== | ===Function=== | ||
| − | + | * '''''TCD9''''' encoding α-subunit of Cpn60 protein (Cpn60α), | |
| + | * '''''TCD9''''' plays a crucial role in chloroplast development at early leaf stage of rice. | ||
| + | |||
| + | ===Phenotypic analysis=== | ||
| + | * The leaves of tcd9 mutant appeared albino phenotype before the 3-leaf stage when grown below 24 ◦C, but the 4th leaves gradu- ally turned green and appeared normal green after the 5-leaf stage even at 20 ◦ C (Fig. 1A). | ||
| + | * The color and height of tcd9 mutant was no discrepancy with wild type when grown beyond 28 ◦C (Fig. 1A). Similarly, the contents of Chl a, Chl b, total Chl and carotenoid (Car) in tcd9 plants before the 3-leaf stage at 20 ◦C were drastically lower than those in the wild type plants and itself grown beyond 28 ◦C(Suppl. Table S3); however, the contents were comparable between wild type plants and tcd9 plant grown at 32 ◦ C (Fig. 1B). | ||
| + | * The observations suggested the thermo-sensitivity of albino phenotype in the mutant as 7436S, Fan5, v1, v2 and v3 | ||
===Expression=== | ===Expression=== | ||
| − | + | * qRT-PCR analysis showed that '''''TCD9''''' exhibited the high expression in all tested tissues,especially in young leaves. | |
| + | |||
| + | ===Subcellular localization=== | ||
| + | * Subcellular localization analysis showed that the GFP fluorescence was colocalized with chlorophyll autofluorescence, showing that '''''TCD9''''' was a chloroplast-localized protein. | ||
| − | |||
| − | |||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China | |
| + | * The Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 12 South Zhong-Guan Cun Street, Beijing 100081, China | ||
| + | * Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China | ||
==References== | ==References== | ||
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Jiang Q, Mei J, Gong XD, Xu JL, Zhang JH, Teng S, Lin DZ, Dong YJ. Importance | ||
| + | of the rice TCD9 encoding α subunit of chaperonin protein 60 (Cpn60α) for the | ||
| + | chloroplast development during the early leaf stage. Plant Sci. 2014 | ||
| + | Feb;215-216:172-9. doi: 10.1016/j.plantsci.2013.11.003. Epub 2013 Nov 18. PubMed | ||
| + | PMID: 24388528. | ||
| + | </ref> | ||
| + | |||
| + | </references> | ||
==Structured Information== | ==Structured Information== | ||
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 9]] | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 9]] | ||
Latest revision as of 09:27, 12 October 2016
The rice Os09g0563300 was reported as TCD9 in 2014 [1] by researchers from China.
Contents
Annotated Information
Figure 6. Subcellular localization of pMON530-SPTCD9-GFP protein.[1].
Gene Symbol
- Os09g0563300 <=> OsTCD9,TCD9
Function
- TCD9 encoding α-subunit of Cpn60 protein (Cpn60α),
- TCD9 plays a crucial role in chloroplast development at early leaf stage of rice.
Phenotypic analysis
- The leaves of tcd9 mutant appeared albino phenotype before the 3-leaf stage when grown below 24 ◦C, but the 4th leaves gradu- ally turned green and appeared normal green after the 5-leaf stage even at 20 ◦ C (Fig. 1A).
- The color and height of tcd9 mutant was no discrepancy with wild type when grown beyond 28 ◦C (Fig. 1A). Similarly, the contents of Chl a, Chl b, total Chl and carotenoid (Car) in tcd9 plants before the 3-leaf stage at 20 ◦C were drastically lower than those in the wild type plants and itself grown beyond 28 ◦C(Suppl. Table S3); however, the contents were comparable between wild type plants and tcd9 plant grown at 32 ◦ C (Fig. 1B).
- The observations suggested the thermo-sensitivity of albino phenotype in the mutant as 7436S, Fan5, v1, v2 and v3
Expression
- qRT-PCR analysis showed that TCD9 exhibited the high expression in all tested tissues,especially in young leaves.
Subcellular localization
- Subcellular localization analysis showed that the GFP fluorescence was colocalized with chlorophyll autofluorescence, showing that TCD9 was a chloroplast-localized protein.
You can also add sub-section(s) at will.
Labs working on this gene
- Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China
- The Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 12 South Zhong-Guan Cun Street, Beijing 100081, China
- Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
References
- ↑ 1.0 1.1 Jiang Q, Mei J, Gong XD, Xu JL, Zhang JH, Teng S, Lin DZ, Dong YJ. Importance of the rice TCD9 encoding α subunit of chaperonin protein 60 (Cpn60α) for the chloroplast development during the early leaf stage. Plant Sci. 2014 Feb;215-216:172-9. doi: 10.1016/j.plantsci.2013.11.003. Epub 2013 Nov 18. PubMed PMID: 24388528.
