Difference between revisions of "Os10g0392600"
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| − | + | The rice '''''Os10g0392600''''' was reported as '''''OsSPX3''''' in 2009 <ref name="ref1" /> by researchers from China. | |
==Annotated Information== | ==Annotated Information== | ||
| + | ===Gene Symbol=== | ||
| + | *'''''Os10g0392600''''' '''<=>''' '''''OsSPX3,SPX3''''' | ||
===Function=== | ===Function=== | ||
* The rice (Oryza sativa L.) genome contains at least six genes exclusively with an SPX (SYG1/PHO81/XPR1) domain at the N-terminal, designated as '''''OsSPX1-6'''''. | * The rice (Oryza sativa L.) genome contains at least six genes exclusively with an SPX (SYG1/PHO81/XPR1) domain at the N-terminal, designated as '''''OsSPX1-6'''''. | ||
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* The transcript level of OsSPX1-3 was higher in root, leaf and callus than in stem, panicle and seed. | * The transcript level of OsSPX1-3 was higher in root, leaf and callus than in stem, panicle and seed. | ||
* The transcript level of OsSPX6 in root and leaf was much higher than in other tissues. RT-PCR analysis indicates that except for OsSPX4, other SPX genes were induced by Pi-starvation in shoot and/or in root | * The transcript level of OsSPX6 in root and leaf was much higher than in other tissues. RT-PCR analysis indicates that except for OsSPX4, other SPX genes were induced by Pi-starvation in shoot and/or in root | ||
| − | === | + | |
| + | ===Subcellular Localization=== | ||
* '''''OsSPX1''''' and '''''OsSPX2 '''''were localized exclusively to the nucleus, and OsSPX4 to membrane (Figure 4H,I,K), while fluorescence of OsSPX3-GFP was present in onion cells as many fluorescent speckles in the cytoplasm (Figure 4J). | * '''''OsSPX1''''' and '''''OsSPX2 '''''were localized exclusively to the nucleus, and OsSPX4 to membrane (Figure 4H,I,K), while fluorescence of OsSPX3-GFP was present in onion cells as many fluorescent speckles in the cytoplasm (Figure 4J). | ||
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| + | [[File:238-Os10g0392600.png|center|thumb|727px|'''Snapshot Figure 4.''' ''Histochemical analysis and subcellular localization of the OsSPX proteins.<ref name="ref1" />.'']] | ||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
Latest revision as of 02:14, 17 February 2017
The rice Os10g0392600 was reported as OsSPX3 in 2009 [1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os10g0392600 <=> OsSPX3,SPX3
Function
- The rice (Oryza sativa L.) genome contains at least six genes exclusively with an SPX (SYG1/PHO81/XPR1) domain at the N-terminal, designated as OsSPX1-6.
- Among them, five genes, OsSPX1, OsSPX2, OsSPX3, OsSPX5 and OsSPX6 are responsive to Pi-starvation in shoots and/or in roots.
- OsSPX1 regulates OsSPX2, OsSPX3 and OsSPX5 at the transcription level.
- OsSPX3 downregulates OsIPS1 and suppresses the responses of OsmiR399 and OsPHO2 to Pi-starvation.
- Whether OsSPX4 functions as ScPHO81 in Pi-signaling pathway on plant Pi-starvation tolerance is an interesting question.
- Overexpression of OsSPX3 inhibits plant growth
Expression
- Except for OsSPX5, the expression was detected only in roots and callus, a constitutive expression pattern was observed in five other genes.
- The transcript level of OsSPX1-3 was higher in root, leaf and callus than in stem, panicle and seed.
- The transcript level of OsSPX6 in root and leaf was much higher than in other tissues. RT-PCR analysis indicates that except for OsSPX4, other SPX genes were induced by Pi-starvation in shoot and/or in root
Subcellular Localization
- OsSPX1 and OsSPX2 were localized exclusively to the nucleus, and OsSPX4 to membrane (Figure 4H,I,K), while fluorescence of OsSPX3-GFP was present in onion cells as many fluorescent speckles in the cytoplasm (Figure 4J).
Snapshot Figure 4. Histochemical analysis and subcellular localization of the OsSPX proteins.[1].
You can also add sub-section(s) at will.
Labs working on this gene
- State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China
