Difference between revisions of "Os07g0498800"

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(Created page with "{{JaponicaGene| GeneName = Os07g0498800| Description = Calreticulin interacted protein| Version = NM_001066246.1 GI:115472224 GeneID:4343305| Length = 8474 bp| Definition...")
 
(Structured Information)
 
(2 intermediate revisions by 2 users not shown)
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{{JaponicaGene|
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'''''CRTintP1''''' '''confers cold tolerance''' on rice plants<ref name="ref1"/>.
GeneName = Os07g0498800|
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Description = Calreticulin interacted protein|
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==Annotated Information==
Version = NM_001066246.1 GI:115472224 GeneID:4343305|
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===Function===
Length = 8474 bp|
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CDPK13, calreticulin and CRTintP1 '''might''' be '''important signaling components''' for '''response to cold stress''' in rice<ref name="ref1"/>. CRTintP1 was identiWed as a calreticulin-interacting protein by yeast two-hybrid system and was '''binding to calreticulin''' using immuno-precipitation system, which indicating that '''CDPK13, calreticulin and CRTintP1 were related with each others'''<ref name="ref2"/>.
Definition = Oryza sativa Japonica Group Os07g0498800, complete gene.|
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Source = Oryza sativa Japonica Group
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'''GO assignment(s):''' [http://amigo.geneontology.org/amigo/term/GO:0006464 GO:0006464]
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===Mutation===
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Transgenic rice<ref name="ref1"/>:
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*'''Accumulation of calreticulin protein''' and '''CRTintP1 protein''' in the sense calreticulin and CRTintP1 transgenic rice was '''higher''' than those in vector control transgenic rice, respectively.
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*Calreticulin protein in sense CRTintP1 transgenic rice and CRTintP1 in sense calreticulin transgenic rice were also higher than that in the vector-control transgenic rice. These data demonstrate the interaction of calreticulin with CRTintP1 in vivo, which is in agreement with the observations obtained from the yeast two-hybrid analysis.
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===Expression===
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*Northern and immunoblot analysis showed increased expression of calreticulin and CRTintP1 in '''response to cold stress'''. Co-immunoprecipitation using anti-calreticulin antibodies confirmed the existence of the calreticulin- CRTintP1 complex in vivo in the stressed '''leaf tissue'''<ref name="ref3"/>.
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*CRTintP1 was identified as a calreticulin-interacting protein by yeast two-hybrid systems and was found bound to calreticulin using immono-precipitation system. The interaction of calreticulin with CRTintP1 in vivo, which is in agreement with the observations obtained from the yeast two-hybrid analysis<ref name="ref2"/><ref name="ref3"/>.
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===Subcellular localization===
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Cellular localization studies showed that a fusion of CRTintP1 to green fluorescent protein was directed to the '''nucleus in onion epidermal cells'''<ref name="ref2"/>.
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===Knowledge Extension===
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*Calreticulin (CRT), a major Ca<sup>2+</sup>-sequestering protein, has been implicated in a variety of cellular functions such as Ca<sup>2+</sup> storage, signaling and chaperone activity within the cytoplasm and endoplasmic reticulum. To investigate the biological role of CRT in rice, 21 partial cDNAs, encoding proteins that interacted with rice CRT in a yeast two-hybrid interaction-cloning system, were characterized and the nucleotide sequences were found to be identical to each other<ref name="ref2"/>.
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*Northern and immunoblot analysis showed increased expression of CRT and CRTintP in response to cold stress. Co-immunoprecipitation using anti-CRT antibodies confirmed the existence of the CRT-CRTintP complex in vivo in the stressed leaf tissue, suggesting their potential role in regulating stress response<ref name="ref2"/>.
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==Labs working on this gene==
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*National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba 305-8518, Japan
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*National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan
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*National Agricultural Research Center for Tohoku Region, Akita 014-0102, Japan
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==References==
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<references>
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* <ref name="ref1">
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Komatsu S, Yang G, Khan M, et al. Over-expression of calcium-dependent protein kinase 13 and calreticulin interacting protein 1 confers cold tolerance on rice plants[J]. Molecular Genetics and Genomics, 2007, 277(6): 713-723.
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</ref>
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* <ref name="ref2">
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Sharma A, Isogai M, Yamamoto T, et al. A novel interaction between calreticulin and ubiquitin-like nuclear protein in rice[J]. Plant and cell physiology, 2004, 45(6): 684-692.
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</ref>
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* <ref name="ref3">
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Komatsu S. Rice proteome database: a step toward functional analysis of the rice genome[J]. Plant molecular biology, 2005, 59(1): 179-190.
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</ref>
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</references>
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==Structured Information==
  
  ORGANISM  Oryza sativa Japonica Group
 
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
 
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
 
            clade; Ehrhartoideae; Oryzeae; Oryza.
 
|
 
Chromosome = [[:category:Japonica Chromosome 7|Chromosome 7]]|
 
AP = Chromosome 7:19377339..19385812|
 
CDS = 19377627..19377701,19378023..19378178,19378430..19379032,19379303..19379631,19379729..19380292<br>,19380865..19380979,19381063..19381134,19381217..19381381,19382341..19382489<br>,19382567..19382636,19382712..19382858,19382936..19383004,19383274..19383314<br>,19383859..19383957,19384519..19384636,19384814..19384942|
 
GCID = <gbrowseImage1>
 
name=NC_008400:19377339..19385812
 
source=RiceChromosome07
 
preset=GeneLocation
 
</gbrowseImage1>|
 
GSID = <gbrowseImage2>
 
name=NC_008400:19377339..19385812
 
source=RiceChromosome07
 
preset=GeneLocation
 
</gbrowseImage2>|
 
CDNA = <cdnaseq>atgtctgaagatgcgtctgtcggggccagcagttcaacagtaaaagctggcgatgatccagaggctaccattgagatcaacatcaaaaccctggattcacaagttcataagctccgtgttaagaagaatgtacctgttctggtccttaaagagaagatagtagaggcaaccggggttcctgtggaccaacagcggttgatttttagaggaagagtcttaaaggacgatcacctgctatcagaatatcatttggaagatgggtacacattgcatttggttgctcggcgtgcagctgctgaaggccaacattcttctggtacttctgatgaaaacacccatgctaatgttaatgttgctggaaatggactgttaggagatatctccaggagtgttcgggatatccttggctccctaggtcttgcgacgcctggtggcatgacaaacactacattttcggtgcctttaaccactgcaccagaaggggccaataatgtcaatggaagaactcaaccagggaatcatgcacaaccagggttttcaattctgaatcatcaaatccaagtatcacaactacaaccagcaggctctattcctcgcaacatggttattcctgattctctcacaactcttttggagtatatcaaccgcatggatcaagtactacagaataacggcacaccatctgttgatacaaatacccagcagccaccaagatcggatgatgcttatctaaatcaaagatttccaagtcctgaggttctggtgtcagtcattgaaagagcccaacaacttcttggtggcagtgctgcttctgctctttcacatctcgcacaacgaatccagagagattctggcaccagtgatgcatctatacgtagccagatccagaatgaatcagctcagctgggagtagcaatgcagcatttgggtgcaatgtttttggagcttggtcgaacaatgatgatgcttcggatggggccatcccctgctgatgcttttgtcaatgctggatcttctgtttatataaactctgcaggaccgaatccaatcatggttcagccatcttttcaaaatactcctccttttggagtttcgagcataccagtcctcggtggaatttctggtgcctttggtattgttgatccttctcgtacatctgctgtcaatacccatggtacttctacaacaagtgggtcatctgctggtatgaccactgcttctgcaggcgctgtcaatgaaggtcgtcaaaatgtggaaagaactcaaggaggtaacccatctgctacctcaatgcatggattgccagcaaggacagttattgcggctattcctgcacggtccacggctgaggctccaaaccatgtcctgagtgttatcctgcctgttcaagtgaggagtcaagtagcaatgcctaatcaatcaacagtttctcaaggttctcagactgcagtgggcggtggatctcaaccacaagcctctgtaggtggtgttgctagcattccttctatagtggcacaggtaactgcacaagtagccaatgcattgagtgcaaatcaacaaggccaggtttcatcatctgcgcagaacacagtagatcagggatctcgctcggtcacaactaatggagttgacaatgtggattctctggtatcagcaagtacgcaactgcaaaatgagctgtcagattctaacaatggacgcacttcactaaatgcacaatctcttgtagctggagcaggtatttctccttcaaacacatctgatcccaatttagcatcggaagacagcagcactgaaaatgctcccaacatcggcagtattcaacaacatccagagatggaagggatccatgcagataatgtcagaaaaccttctgaggaatcaacaacagccaacttagtggggcagatcacaaccacctgtacagatgatatttctgtgaacagatcagcagaaaattcttcacagaagaacattccattggatggagtatctgcacagtccattaagccatctgcaagtagtaggtctgaaccagtaggtcttggtggaggtttgcagcctaagaggcggagcagaacagcaaagccacctgggagtagcagtgatactggcgaagtcgtcaattcctctcgcatcagcaatagccaaaatgctgtttcaatgggccagcaggttctgcaagcccttgcttctcaaaatactaatgtaaacagaagccatgttacggattctccacttccatccactacttctcagttttctggtggaatgcctccgagaagacagggtggtgaaggacaagttgattttggcagtatgatatccagtgtgctaaacaacccagcttttggcaatctgttgtccaatgtagcagagcaaacaggcatgggttccgcaggtgatttgagaaacatggtggaagagtgtgcacagagccctgcaataatggatactatgagtaatttagtccaaaatgtggatgggtcaggaagaggtcaaggtggcattgacttgtctagaatgatgcagcaaatgatgcctgttgtatcccaagttcttggtggagctggggctcgtcctgctggtacaaatagtggacaatccagattgcagcctcggcgcagtgacatgagagtggatgatgcttcagattatggaaattctcagattgatctacaccaagctcgtgaacacattgagcaacatgactcccccagggatatcttcggtgcggtcctcgaaactgctgcacaggcttatggtgaagatgagagtattgaggacatgcttgaagagcttgtcagtgacccagaacttacagatgactacctgaaacttctgctccaacaagttcgccagaggatacagtcggcatctcaatccgggaaccagtcttga</cdnaseq>|
 
AA = <aaseq>MSEDASVGASSSTVKAGDDPEATIEINIKTLDSQVHKLRVKKNV                    PVLVLKEKIVEATGVPVDQQRLIFRGRVLKDDHLLSEYHLEDGYTLHLVARRAAAEGQ                    HSSGTSDENTHANVNVAGNGLLGDISRSVRDILGSLGLATPGGMTNTTFSVPLTTAPE                    GANNVNGRTQPGNHAQPGFSILNHQIQVSQLQPAGSIPRNMVIPDSLTTLLEYINRMD                    QVLQNNGTPSVDTNTQQPPRSDDAYLNQRFPSPEVLVSVIERAQQLLGGSAASALSHL                    AQRIQRDSGTSDASIRSQIQNESAQLGVAMQHLGAMFLELGRTMMMLRMGPSPADAFV                    NAGSSVYINSAGPNPIMVQPSFQNTPPFGVSSIPVLGGISGAFGIVDPSRTSAVNTHG                    TSTTSGSSAGMTTASAGAVNEGRQNVERTQGGNPSATSMHGLPARTVIAAIPARSTAE                    APNHVLSVILPVQVRSQVAMPNQSTVSQGSQTAVGGGSQPQASVGGVASIPSIVAQVT                    AQVANALSANQQGQVSSSAQNTVDQGSRSVTTNGVDNVDSLVSASTQLQNELSDSNNG                    RTSLNAQSLVAGAGISPSNTSDPNLASEDSSTENAPNIGSIQQHPEMEGIHADNVRKP                    SEESTTANLVGQITTTCTDDISVNRSAENSSQKNIPLDGVSAQSIKPSASSRSEPVGL                    GGGLQPKRRSRTAKPPGSSSDTGEVVNSSRISNSQNAVSMGQQVLQALASQNTNVNRS                    HVTDSPLPSTTSQFSGGMPPRRQGGEGQVDFGSMISSVLNNPAFGNLLSNVAEQTGMG                    SAGDLRNMVEECAQSPAIMDTMSNLVQNVDGSGRGQGGIDLSRMMQQMMPVVSQVLGG                    AGARPAGTNSGQSRLQPRRSDMRVDDASDYGNSQIDLHQAREHIEQHDSPRDIFGAVL                    ETAAQAYGEDESIEDMLEELVSDPELTDDYLKLLLQQVRQRIQSASQSGNQS</aaseq>|
 
DNA = <dnaseqindica>8112..8186#7635..7790#6781..7383#6182..6510#5521..6084#4834..4948#4679..4750#4432..4596#3324..3472#3177..3246#2955..3101#2809..2877#2499..2539#1856..1954#1177..1294#871..999#agttctcacttctcacacaactcgcaaccctaataaagcgcaaaaaaggaaaaagaaaaatcgccacggaaaaatcctcgctgccccccatcgggcaggagcaaccgccgcatcggcccccgatccggtcgccgccgcctctcgcccggttcatccgccgcttgccaacccctccgctcccagcaaccctagtcccccaccccaacgccgccgccagctcagctcgcctcgcgcggggcgcagccgactctctacttgtggcctcggcggctcgggccctctccggattccacgtatggctcgatcttctgcttttctttttcttctttttttttttttaattattcgggaattgaagcctcatcctattgggggcatacgcgacttggcggagaggacaatggcgacgcatttcagagctaaggggggaagggatatgattttgaggctgtaattgcttgcgaagttctccttttggaagggatcggtcctagccattctgagctgctaaaaattttatttgcggtacggagcgtcttctgataagaggacagaacgattagggtctcccgtaatctgcttttaaatgcttgatggagtcctagaacctaaaattatggaattttctctaatgttaagctaggaatgagggtactggtttcttcgtagatggaaggcgctctacttgttgctgccggaactctgttgagatcttgtgcttaaatgcaggggtttaactggctgtgtaatcttaggcgtggagtgagttgactatgaagaattctgaacatgtctaggaatcataatttcgaattttgtagcttgtggtttatgcagcatctgatactattcagttcctcgttctgtaggatgtctgaagatgcgtctgtcggggccagcagttcaacagtaaaagctggcgatgatccagaggctaccattgagatcaacatcaaaaccctggattcacaagttcataagctccgtgttaagaagaatgtatgcttcgccccaccttgacagtttgctattatgtctctgtgtgtctgttgagtgttgcttgcttgaagagatactagaaatgctatagaatgtcagttccttatagttagaacgacaagatgaattctcccatgtacttgttttgagacaatctaatcactgaaaaatttttaggtacctgttctggtccttaaagagaagatagtagaggcaaccggggttcctgtggaccaacagcggttgatttttagaggaagagtcttaaaggacgatcacctgctatcagaatatcgtatcctttttgttcatgcttcttcttttgaatgcttgaagacaatcaaaccatctagagactcacctcgcttgttgctgttgagaacttgagctatatacactatttacctgcattttgtttcccatctttagttgcttagttataaataggccgggaagatacacaactcttttgggccagtaagggtgatttcgttactgctagaagagtagagtgcctgatgcttttgttgggattagggctgcttgctttggtagtgatttcataatggccttccaatgagacaaaataaagtagtaaaatgtcaaattgaagtatctaagcattaattctttgcaaatatgggcaagaacctcaaatcattgtagaccttgatgatatttgttaagttgaggtggttgaacaccagtatttaactgcatcgttggtggtaatgaggggttccttggatcaacgattttcatcaccacatgatgtaatttatcattttcggttttcttgtgatatttcgtttgtaaacacgacagttgtgatagttatatctttgacaacctcaagatttggaagatgggtacacattgcatttggttgctcggcgtgcagctgctgaaggccaacattcttctggtacttctgatgaaaacacccatgctaatggtaagttaagttgggggttgctttgaagagtatactatcaatatagaacgcacctgatatgcctatgacctattagtcctcagagccatgtgctgcacaagttttcgtagtatcgtaaacaatttatgtgcgttttcactcttcaataccatgtttccatgtgtctgttctatatattaattgtgtcaagctcatactggataaaatattattgccctttgaagggcagaaaaatagttattgctagttattgtaaaaaatagctaggtaagcatttcaggctaagtagatgaaggcttgaacagtccttcgctttatttttcatttaatttgtgaactaaaaacaccattatttaactgtatttatgtaagcattactgtgtttctagttattacaatatatagatagagcataacaaatcaacttgggttatattgactggcactaccttttgtggtaccagagtgtatttcctctgtacctgattttaggtatattacagcaatatgctgggtatgggtctgataatctttttacgtgcagttaatgttgctggaaatggactgttaggagatatctccagggtatgcttctaacatcgctttgtctcgtatacttaaaatttccaatctgcttaactcttagcaacaactacaggttaactttaattttctttgaattctttttcattaactctcttctaggaaattatagaggctatgcattctgaggggatgatagatgacctggctagggtatatgctttgttctagtgttatatgtacctgtctaaatggggaccagaacctttatatgtttattgatgtgatttcctcccacctgttatctgcagagtgttcgggatatccttggctccctaggtcttgcgacgcctggtggcatgacaaacactacattttcggtaataaggtgttacttctttaggtctagtaacctgagacatctcggtatttgacttctatatgtttacttgattaggtgcctttaaccactgcaccagaaggggccaataatgtcaatggaagaactcaaccagggaatcatgcacaaccagggttttcaattctgaatcatcaaatccaagtatcacaactacaaccagcaggctctattcctcgcaacatggttagtaaactgtacttttttcattggtgttttcccatgtataatcatcattataacttttagcaccattgacaggttattcctgattctctcacaactcttttggagtatatcaaccgcatggatcaagtactacagaataacggtatcgtttgttgcaatttgttagtttgatgtgtgtcataaaatactgtcatgttcatctttatgttgcaaattcaggcacaccatctgttgatacaaatacccagcagccaccaagatcggatgatgcttatctaaatcaaagatttccaagtcctgaggttctggtgtcagtcattgaaagagcccaacaacttcttggtggcagtgctgcttctgctctttcagtatgttatcctctctggtacttatattaagttctttttagcttcagtggttcagcctttatagtgatatcttgtcgacgtatagtttatccttctttgtaagtactatattttgtaatgattttgccttctttctaatcagcaaaataattctctttgaacatcaatatgttgccaatgtactacttttcagttctcttatttgagacaaaactatggagatttaaaccaaaataaatccattgtcccattccgtgaccataatcctgctcacagttatctctaatggaccatatacatttaatggctgatactacttgttacaatatagatgattaggctttataacaactactaggaggaacttttagccacttcgtggtacaaaaataacactctagtgttgtgttttgaatataatttttgttttgccttgggtactaggtctgccttagtgccgtgtaacactgctctatattatccctgaatattatatgaggattcaaatggtatttgtaaacgttttgtaccaccactctgcattatccctgaatattatatgagaattcaaatggtatttataaacaccagatgttatttgtttgagagactgatgatctgtttttcattcttttctgcactatccctgctaacattttcctttgggtgccgtacacttgatctgtttttcattcttctctagcattgattgacatgtgaaaggtctaagtggacctctcaaaggtatcaatgggacattagtcttcctagttcggttcttttctctttttttattatgatacccacatttcatacttctcaacgcttattgattcaaaggggtcatatgtacgcagcttcaagactgagtacagtgaaatagcttacctttgaattcacagacgcatgcttctatttggaattttgatgaccttctgtttacctgatatttgtttcagcatctcgcacaacgaatccagagagattctggcaccagtgatgcatctatacgtagccagatccagaatgaatcagctcagctgggagtagcaatgcagcatttgggtgcaatgtttttggagcttggtcgaacaatgatgatgcttcggatggggccatcccctgtatgcttaatttaaattctctattatagtatttaactgatttctgcatgtgttaatttcataacgcttcttattttggtaggctgatgcttttgtcaatgctggatcttctgtttatataaactctgcaggaccgaatccaatcatggttcaggtgacatacattgagatgctttctcatgttgactttaattgttgtgccactttaatgacaac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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001066246.1 RefSeq:Os07g0498800]|
 
}}
 
 
[[Category:Genes]]
 
[[Category:Genes]]
 
[[Category:Japonica mRNA]]
 
[[Category:Japonica mRNA]]

Latest revision as of 08:40, 12 June 2015

CRTintP1 confers cold tolerance on rice plants[1].

Annotated Information

Function

CDPK13, calreticulin and CRTintP1 might be important signaling components for response to cold stress in rice[1]. CRTintP1 was identiWed as a calreticulin-interacting protein by yeast two-hybrid system and was binding to calreticulin using immuno-precipitation system, which indicating that CDPK13, calreticulin and CRTintP1 were related with each others[2].


GO assignment(s): GO:0006464

Mutation

Transgenic rice[1]:

  • Accumulation of calreticulin protein and CRTintP1 protein in the sense calreticulin and CRTintP1 transgenic rice was higher than those in vector control transgenic rice, respectively.
  • Calreticulin protein in sense CRTintP1 transgenic rice and CRTintP1 in sense calreticulin transgenic rice were also higher than that in the vector-control transgenic rice. These data demonstrate the interaction of calreticulin with CRTintP1 in vivo, which is in agreement with the observations obtained from the yeast two-hybrid analysis.

Expression

  • Northern and immunoblot analysis showed increased expression of calreticulin and CRTintP1 in response to cold stress. Co-immunoprecipitation using anti-calreticulin antibodies confirmed the existence of the calreticulin- CRTintP1 complex in vivo in the stressed leaf tissue[3].
  • CRTintP1 was identified as a calreticulin-interacting protein by yeast two-hybrid systems and was found bound to calreticulin using immono-precipitation system. The interaction of calreticulin with CRTintP1 in vivo, which is in agreement with the observations obtained from the yeast two-hybrid analysis[2][3].

Subcellular localization

Cellular localization studies showed that a fusion of CRTintP1 to green fluorescent protein was directed to the nucleus in onion epidermal cells[2].

Knowledge Extension

  • Calreticulin (CRT), a major Ca2+-sequestering protein, has been implicated in a variety of cellular functions such as Ca2+ storage, signaling and chaperone activity within the cytoplasm and endoplasmic reticulum. To investigate the biological role of CRT in rice, 21 partial cDNAs, encoding proteins that interacted with rice CRT in a yeast two-hybrid interaction-cloning system, were characterized and the nucleotide sequences were found to be identical to each other[2].
  • Northern and immunoblot analysis showed increased expression of CRT and CRTintP in response to cold stress. Co-immunoprecipitation using anti-CRT antibodies confirmed the existence of the CRT-CRTintP complex in vivo in the stressed leaf tissue, suggesting their potential role in regulating stress response[2].

Labs working on this gene

  • National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba 305-8518, Japan
  • National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan
  • National Agricultural Research Center for Tohoku Region, Akita 014-0102, Japan

References

  1. 1.0 1.1 1.2 Komatsu S, Yang G, Khan M, et al. Over-expression of calcium-dependent protein kinase 13 and calreticulin interacting protein 1 confers cold tolerance on rice plants[J]. Molecular Genetics and Genomics, 2007, 277(6): 713-723.
  2. 2.0 2.1 2.2 2.3 2.4 Sharma A, Isogai M, Yamamoto T, et al. A novel interaction between calreticulin and ubiquitin-like nuclear protein in rice[J]. Plant and cell physiology, 2004, 45(6): 684-692.
  3. 3.0 3.1 Komatsu S. Rice proteome database: a step toward functional analysis of the rice genome[J]. Plant molecular biology, 2005, 59(1): 179-190.

Structured Information