Difference between revisions of "Os06g0348800"

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Please input one-sentence summary here.
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The rice '''''Os06g0348800''''' was reported as '''''OsGLK1''''' in 2009 <ref name="ref1" /> by researchers from Japan.  
  
 
==Annotated Information==
 
==Annotated Information==
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===Gene Symbol===
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*'''''Os06g0348800''''' '''''<=>''''' '''''OsGLK1,GLK1'''''
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===Function===
 
===Function===
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Please input function information here.
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* '''''OsGLK1''''' regulates chloroplast development under the control of light and phytohormones, and that it is a key regulator of chloroplast development.
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===Phenotypic analysis===
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* The rice expression-microarray and northern blot analyses, showd that a large number of nucleus-encoded genes involved in chloroplast functions were highly expressed and transcripts of plastid-encoded genes, psaA , psbA and rbcL , increased in the '''''OsGLK1'''''-FOX calli.
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* Transmission electron microscopy showed the existence of differentiated chloroplasts with grana stacks in '''''OsGLK1''''' -FOX calli cells.
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* However, in darkness, '''''OsGLK1'''''-FOX calli did not show a green color or develop grana stacks.
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* Developed chloroplasts was found in vascular bundle and bundle sheath cells of coleoptiles and leaves from '''''OsGLK1'''''-FOX seedlings.
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* The '''''OsGLK1'''''-FOX calli exhibited high photosynthetic activity and were able to grow on sucrose-depleted media, indicating that developed chloroplasts in '''''OsGLK1'''''-FOX rice calli are functional and active.
  
 
===Expression===
 
===Expression===
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Please input expression information here.
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* the endogenous OsGLK1 mRNA level was observed to be increased synchronously with the greening of wild-type calli after transfer to plantlet regeneration medium.
  
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===Evolution===
 
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You can also add sub-section(s) at will.
 
You can also add sub-section(s) at will.
  
 
==Labs working on this gene==
 
==Labs working on this gene==
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Please input related labs here.
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* Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602 Japan
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* Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602 Japan
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* Department of Applied Biological Chemistry, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan
  
 
==References==
 
==References==
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Please input cited references here.
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<references>
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* <ref name="ref1">
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Nakamura H, Muramatsu M, Hakata M, Ueno O, Nagamura Y, Hirochika H, Takano M,
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Ichikawa H. Ectopic overexpression of the transcription factor OsGLK1 induces
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chloroplast development in non-green rice cells. Plant Cell Physiol. 2009
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Nov;50(11):1933-49. doi: 10.1093/pcp/pcp138. PubMed PMID: 19808806; PubMed
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Central PMCID: PMC2775961.
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</ref>
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</references>
 
==Structured Information==
 
==Structured Information==
 
     [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 6]]
 
     [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 6]]

Latest revision as of 12:41, 2 March 2017

The rice Os06g0348800 was reported as OsGLK1 in 2009 [1] by researchers from Japan.

Annotated Information

Gene Symbol

  • Os06g0348800 <=> OsGLK1,GLK1

Function

  • OsGLK1 regulates chloroplast development under the control of light and phytohormones, and that it is a key regulator of chloroplast development.

Phenotypic analysis

  • The rice expression-microarray and northern blot analyses, showd that a large number of nucleus-encoded genes involved in chloroplast functions were highly expressed and transcripts of plastid-encoded genes, psaA , psbA and rbcL , increased in the OsGLK1-FOX calli.
  • Transmission electron microscopy showed the existence of differentiated chloroplasts with grana stacks in OsGLK1 -FOX calli cells.
  • However, in darkness, OsGLK1-FOX calli did not show a green color or develop grana stacks.
  • Developed chloroplasts was found in vascular bundle and bundle sheath cells of coleoptiles and leaves from OsGLK1-FOX seedlings.
  • The OsGLK1-FOX calli exhibited high photosynthetic activity and were able to grow on sucrose-depleted media, indicating that developed chloroplasts in OsGLK1-FOX rice calli are functional and active.

Expression

  • the endogenous OsGLK1 mRNA level was observed to be increased synchronously with the greening of wild-type calli after transfer to plantlet regeneration medium.


You can also add sub-section(s) at will.

Labs working on this gene

  • Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602 Japan
  • Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602 Japan
  • Department of Applied Biological Chemistry, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan

References

  1. ↑ Nakamura H, Muramatsu M, Hakata M, Ueno O, Nagamura Y, Hirochika H, Takano M, Ichikawa H. Ectopic overexpression of the transcription factor OsGLK1 induces chloroplast development in non-green rice cells. Plant Cell Physiol. 2009 Nov;50(11):1933-49. doi: 10.1093/pcp/pcp138. PubMed PMID: 19808806; PubMed Central PMCID: PMC2775961.

Structured Information