Difference between revisions of "Os01g0907400"

Revision as of 14:36, 9 October 2016

The rice Os01g0907400 was reported as JMJ705 in 2013 ^[1] by researchers from China and France.

JMJ705 encodes a histone lysine demethylase that specifically reverses H3K27me2/3.
Overexpression of JMJ705 reduces the resting level of H3K27me2/3 resulting in preferential activation of H3K27me3-marked biotic stress responsive genes and enhances rice resistance to the bacterial blight disease pathogen Xanthomonas oryzae pathovar oryzae.
Mutation of JMJ705 reduces plant resistance to the pathogen.
JMJ705 is involved in methyl jasmonate–induced dynamic removal of H3K27me3 and gene activation.
The results suggest that JMJ705 is a biotic stress-responsive H3K27me2/3 demethylase that may remove H3K27me3 from marked defense-related genes and increase their basal and induced expression during pathogen infection.

To study whether increased expression of JMJ705 could reduce the resting level of H3K27me3 and affect gene expression and plant growth, we produced transgenic rice plants overexpressing JMJ705-FLAG under the strong maize ubiquitin promoter in the Zhonghua11 (ZH11) background (Figure 2A).
Protein gel blot analysis indicated that the overexpression of the gene resulted in a reduction of the overall H3K27me2/3 levels in the transgenic plants compared with wild type (Figure 1D). The overexpression transgenic lines displayed a leaf lesion-mimic phenotype at the mature stage (Figures 2A and 2B).

RT-PCR analysis indicated that JMJ705 was expressed in all tested tissues/organs, with a relatively higher level in rice leaves, and was found to be induced by NaCl, abscisic acid, ethylene (ACC), and JA (see Supplemental Figure 2 online), suggesting that JMJ705 might be involved in stress responses.
The expression of JMJ705 is induced by stress signals and during pathogen infection.

Please input evolution information here.

You can also add sub-section(s) at will.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China
Institut de Biologie des Plantes Unité Mixte de Recherche 8618 Université Paris-sud 11, 91405 Orsay, France

↑ Jumonji C Domain Protein JMJ705-Mediated Removal of Histone H3 Lysine 27 Trimethylation Is Involved in Defense-Related Gene Activation in Rice

@@ Line 10: / Line 10: @@
 * '''''JMJ705''''' is involved in methyl jasmonate–induced dynamic removal of H3K27me3 and gene activation.
 * The results suggest that '''''JMJ705''''' is a biotic stress-responsive H3K27me2/3 demethylase that may remove H3K27me3 from marked defense-related genes and increase their basal and induced expression during pathogen infection.
+===Phenotypic analysis===
+* To study whether increased expression of '''''JMJ705''''' could reduce the resting level of H3K27me3 and affect gene expression and plant growth, we produced transgenic rice plants overexpressing JMJ705-FLAG under the strong maize ubiquitin promoter in the Zhonghua11 (ZH11) background (Figure 2A).
+* Protein gel blot analysis indicated that the overexpression of the gene resulted in a reduction of the overall H3K27me2/3 levels in the transgenic plants compared with wild type (Figure 1D). The overexpression transgenic lines displayed a leaf lesion-mimic phenotype at the mature stage (Figures 2A and 2B).
 ===Expression===
 * RT-PCR analysis indicated that '''''JMJ705''''' was expressed in all tested tissues/organs, with a relatively higher level in rice leaves, and was found to be induced by NaCl, abscisic acid, ethylene (ACC), and JA (see Supplemental Figure 2 online), suggesting that JMJ705 might be involved in stress responses.