Os02g0557200

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Annotated Information

Function

OsARF1, a rice homologue of the auxin response factor (ARF) family of transciptional regulators, is localized to the nuclues. OsARF1, classified as a primary auxin responsive gene, which is auxin-induced even in the absence of de novo protein synthesis, is regulated by auxin. OsARF1, as a transciption factor which can regulate the expresssion of genes is important for growth. In rice, elevated levels of OsARF1 transciption in tissues elevating the growth rate suggest that OsARF1 might play a positive role in the regulation of gene encoding products mediating the growth response. As the P, S, T-rich OsARF1 middle region suggests that OsARF1 acts as a repressor, the positive correlation of growth rate and high OsARF1 transcript levels might be a consequence of OsARF1 negatively regulating a repressor.[1] insertion of a cDNA fragment of OsARF1 produce an antisense (AS) construction, and AS-OsARF1 plant showed exetremely low growth.(Fig.1)[2] The ealier expression of OsARF1 promote plant growth, whereas the later expression (after 12h of colonization) may be related to infection by P. Cepacia. OsARF1 may be at the aerial parts of the plant to interact with the pathogen antagonism.[3] OsARF1 can regulate its own transciption, involving in a feedback mechanism in self-amplification of auxin signaling.[4]

Mutant reported by Yaling Song et al.[5] T-DNA insertion mutant of OsARF1 is less sentitive to BR treatment compared with wild type. Both shoot and root elongation is more significantly inhibited in the homozygous mutant plant than that of wild type. (Fig.2.) (Fig.3.)

Expression

Semi-quantitative RT-PCR showed that the expression level of OsARF1 was extremely low. OsARF1 was transcript accumulated in cellus and young panicle at much higer amount than in leaf and root. OsARF1 may be associated with embryogenesis.[2] Microarray analysis of regulated genes expression by OsARF1 in transgenic plants showed that in later vegetative stages, 10325genes of the total of 14688 genes were expressed at this stage. “knock down” plant cDNA microarray analysis showed that approximately 3% fo the total set of predicted genes and approximately 4% of known to be expressed genes showed significant difference among their transcirptional abundance between the transgenic plant and the controls. [2] (Fig.4.)

Evolution

Dekai Wang et al. built a phylogenetic tree from alignments of the full-length protein sequences of 25 OsARFs to examine the phylogenetic relationships among rice proteins by using MEGA3.1 program.[6] (Fig.5. )

Labs working on this gene

1、Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395; 2、Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara630-0101, Japan; 3、Rice Res & Training Ctr, Rice Biotechnol Lab, Sakha 33717, Kafr E1 Sheikh, Egypt; 4、Fudan Univ, Sch Life Sci, Inst Genet Engn, Shanghai 200433, Peoples R China; 5、Key Laboratory of Monitoring and Management of Plant Pathogens and Insect Pests, Ministry of Agriculture of China; 6、Institute of Botany, University of Karlsruhe, Kaiserstranbe, 76128 Karlsruhe, Germany; 7、Crop Molecular Breeding Center, the Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R China; 8、State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, P.R China; 9、National Center for Gene Research, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, P.R China.

Structured Information

Gene Name	Os02g0557200
Description	Similar to Auxin response factor 1
Version	NM_001053672.1 GI:115446714 GeneID:4329672
Length	5785 bp
Definition	Oryza sativa Japonica Group Os02g0557200, complete gene.
Source	Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome	Chromosome 2
Location	Chromosome 2:21956052..21961836
Sequence Coding Region	21956355..21956403,21957178..21957293,21957517..21957612,21957744..21957800,21958015..21958167 ,21958269..21958353,21958517..21958607,21958746..21958910,21959263..21959382 ,21959474..21959549,21959715..21959886,21960042..21960517,21960598..21960788 ,21960879..21961068
Expression	GEO Profiles:Os02g0557200
Genome Context	<gbrowseImage1> name=NC_008395:21956052..21961836 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1>
Gene Structure	<gbrowseImage2> name=NC_008395:21956052..21961836 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2>
Coding Sequence	<cdnaseq>atggctggctccgtcgttgctgctgctgccgccgccggcggtggcacagggagctcttgcgatgcgttgtacagggagctatggcatgcttgcgctgggccgttggtcacagtgcctcgccaaggcgaattggtttactacttcccccagggccatatggaacagcttgaggcatctacagaccaacagcttgaccagcatctgcctttgtttaacctgccatctaagatcctctgcaaagtggtcaatgtagaacttagggctgaaaccgattcagatgaggtttatgctcaaattatgctgcaaccagaagcagatcaaaatgaactcaccagcccaaaacctgagccacatgaacctgaaaaatgcaatgtccattccttctgcaagactttgactgcttcagatacaagcacccatggtggattttcagttcttaggaggcatgcagaagaatgtcttcctcccctggacatgactcagaatccaccatggcaagagcttgtggctagagatctccatggaaacgagtggcatttccgtcacatctttcgaggacaacctaggaggcatctgcttacaactggctggagtgtttttgttagctcaaaaagattagttgccggtgacgcattcatctttctgagaggtgagaatggagagctacgagttggtgtcaggaggctcatgaggcaactaaataacatgccatcatcagtaatatcaagtcatagtatgcatcttggagtcctagcaactgcatcacatgctatatccaccggaactctcttttctgttttctacaaaccaagaacaagtcagtctgagtttgttgtgagtgctaacaagtaccttgaagctaaaaacagcaagatatctgttggaatgaggtttaagatgagattcgagggcgatgaagctcctgaaagaaggtttagtggaacaattattggtgttgggagcatgtcaacgtctccatgggcaaattctgactggagatctttgaaggtccaatgggatgagccttctgtggttcctcgtccagatagagtttcaccatgggaactagagccacttgctgtgagtaattcacagccatcacctcagccaccggcaagaaataagcgggctcggcctcctgcttcgaattcaattgctccagaactacctccggtcttcggtttgtggaaatcttcagctgagtccacacaaggcttctcattttcgggactacagcgaacacaggaactatatccttcaagccccaatccgatcttctcaacatcattaaatgttggattcagtacaaagaatgagccatctgctctaagcaacaagcatttttactggccaatgagagagacaagagccaactcttactctgctagcatcagcaaagttccatctgaaaagaagcaagagcctagttcggctggctgcagattgtttggaatcgagataagctctgctgtggaagctacatctccactggctgctgtttctggtgttggtcaagaccaaccagctgcctcagtagatgctgagtctgatcagctctcacaaccatcacatgccaacaaatctgatgctccagcggcaagcagtgagccatctcctcatgagactcagagtcggcaagtgaggagctgcaccaaggtcatcatgcaaggaatggcagttggaagggcagtggacctgacaaggctccatggatatgatgatcttcgctgcaagttagaagagatgtttgatatccaaggagagctctctgctagcctaaagaaatggaaggttgtttacacagatgatgaggatgatatgatgctggttggggatgacccttggcctgaattttgcagcatggtgaaaaggatatacatctacacctacgaggaagccaagcaacttactcccaagtcgaagctgccgatcattggcgacgccatcaagccaaacccaaacaaacaatcgcccgagtctgacatgcctcacagtgacctggacagcaccgcccccgtcaccgataaggattgctga</cdnaseq>
Protein Sequence	<aaseq>MAGSVVAAAAAAGGGTGSSCDALYRELWHACAGPLVTVPRQGEL VYYFPQGHMEQLEASTDQQLDQHLPLFNLPSKILCKVVNVELRAETDSDEVYAQIMLQ PEADQNELTSPKPEPHEPEKCNVHSFCKTLTASDTSTHGGFSVLRRHAEECLPPLDMT QNPPWQELVARDLHGNEWHFRHIFRGQPRRHLLTTGWSVFVSSKRLVAGDAFIFLRGE NGELRVGVRRLMRQLNNMPSSVISSHSMHLGVLATASHAISTGTLFSVFYKPRTSQSE FVVSANKYLEAKNSKISVGMRFKMRFEGDEAPERRFSGTIIGVGSMSTSPWANSDWRS LKVQWDEPSVVPRPDRVSPWELEPLAVSNSQPSPQPPARNKRARPPASNSIAPELPPV FGLWKSSAESTQGFSFSGLQRTQELYPSSPNPIFSTSLNVGFSTKNEPSALSNKHFYW PMRETRANSYSASISKVPSEKKQEPSSAGCRLFGIEISSAVEATSPLAAVSGVGQDQP AASVDAESDQLSQPSHANKSDAPAASSEPSPHETQSRQVRSCTKVIMQGMAVGRAVDL TRLHGYDDLRCKLEEMFDIQGELSASLKKWKVVYTDDEDDMMLVGDDPWPEFCSMVKR IYIYTYEEAKQLTPKSKLPIIGDAIKPNPNKQSPESDMPHSDLDSTAPVTDKDC</aaseq>
Gene Sequence	<dnaseqindica>304..352#1127..1242#1466..1561#1693..1749#1964..2116#2218..2302#2466..2556#2695..2859#3212..3331#3423..3498#3664..3835#3991..4466#4547..4737#4828..5017#cctctctctcttcctcttctccccgcgcaagccaaagccacagccagcgaaggcggcaagccaccaccaccacctcctcctcacgccaccgcggcctcctcctccggccaccgcgcgctcacctcgggcagcggccgcagtggcgcgcgctctccggtgtgggatctccgagttttttttttcgttttccctctgaattccgcggcggggaggccggatctgcggggaggggcatggggtgaccagatcgggacgcgcacctcgctctcctcctcctcctcctcctgaattcctctcgtcgggatggctggctccgtcgttgctgctgctgccgccgccggcggtggcacaggttcgtgtcccgcgctttctcttcgcgtttttcgcctcggctggttgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtttggtctgaattggggttgtgaagttagctggtgagtcgagtgcttggtgagctagcaagagagagagagacgtggacttggggggcagttgacttgttcgtctaggggggagagagagaggggttgttgtgtggtgtggaattggttggaattcgaggcgactcggcattgtttaccataaaaagcgccttttctcttccatttcttgtccaattttgtcgtcttgttttcctttttaggtcggagatttgttcctgttgtgctgattcgtgcgaaaactcgtaattccgctagtatggccactgctctagtaattctcttcggatgcaagcgaattccgatctgtgaaatgggggaaacacatgtgcaattacggatctgagaacctgttttgtagtgacagcgtctttgtttctctctcttttttttttgaaaaaaaaatggttaggttggcaattggttctaccacagagacgcaaatgagaaaaatgttggatctatgtgtgtgctgcttgtttagatttacaattctggagcaattatggtgtagttaactcatgatcaacggttaattagacctttttggttgccaccatgacatgaggggaaatttcgcttgattttgtatggtgatgtttttattttcctaaaaaaagagagtaaaaattcctctgtgcagggagctcttgcgatgcgttgtacagggagctatggcatgcttgcgctgggccgttggtcacagtgcctcgccaaggcgaattggtttactacttcccccagggccatatggaacaggtaaatctcactataatatatcaatgcttgcttgctctctatcatgtcctaaaattaaggagtttgctactaatttgtgccataagaatgtcacttgttccttgtgttgacacacagcagtatggttttgtttcttcggcaatgtagttattttagtacgtctttcctttgaactaatcttatgtctttcctttgaactgatcttttcatgctttttctatagcttgaggcatctacagaccaacagcttgaccagcatctgcctttgtttaacctgccatctaagatcctctgcaaagtggtcaatgtagaacttagggtaagcattttccttttctggtctgcagtagtttcacgctatatgaaagttcctctggattttctagcaagagtattacttgtgtacctttcatcctgtagtttactcaccttttttcctctggtatgcaggctgaaaccgattcagatgaggtttatgctcaaattatgctgcaaccagaagcagatgtgagtatgcttttcttatatatttttcttctaaataatagttgtccttttcagctcatttgagctttttatcaagctgtaatgtagtgatcttgctcatgtcatagtcctttctttcttctgatcgtcttctctcatattctcacataaattctattgccttggaattttatatgggtatgtacttattcattttcctttattctgttgttagcaaaatgaactcaccagcccaaaacctgagccacatgaacctgaaaaatgcaatgtccattccttctgcaagactttgactgcttcagatacaagcacccatggtggattttcagttcttaggaggcatgcagaagaatgtcttcctcccctggttaggaccaaaaatatttttaactcccttcatatgcggataatcacattggatatttgctatctgtaacatcttctgtatgtggtatggtttatctttaggacatgactcagaatccaccatggcaagagcttgtggctagagatctccatggaaacgagtggcatttccgtcacatctttcgaggtaagttataggaagaacatagtaatagtgaatgtagttccttctgagcttccataatgaaagggcttccccaaaagaaattggtggcatattagtaatgctttccatggtgattttgttactatgtctcacaaagggtccccccaccactcccttattttaggacaacctaggaggcatctgcttacaactggctggagtgtttttgttagctcaaaaagattagttgccggtgacgcattcatctttctgaggtatgcaggggaacatttggaaggcttgaagccatttaacacattttgcttatttgcgtttgtttcctgggtctgcggagttcaggatgaatattctatatcactattgctgttgtaaatacggtgttcatataacagaggtgagaatggagagctacgagttggtgtcaggaggctcatgaggcaactaaataacatgccatcatcagtaatatcaagtcatagtatgcatcttggagtcctagcaactgcatcacatgctatatccaccggaactctcttttctgttttctacaaaccaaggtttgattcttctaatcttatcattgtacctaagttcttcaccaatgctttatgtacctctagcattttcttatgtacagatgcaatgtacttcgtaataaaatatgcatgatgttatctgatatcctgagatgattccctggatgcccatttaaattttcacttatgatattgctctgttgagtgcttatagaattatatgtaacttttcctcacagaattacattccattttaggaatatatttttgtgtgagtttgtttgataggtcttatttattttcttgtgctaaacatatgttgtaccattacattctaataagtcagtgtttgttcatctaatttgggtttcagaacaagtcagtctgagtttgttgtgagtgctaacaagtaccttgaagctaaaaacagcaagatatctgttggaatgaggtttaagatgagattcgagggcgatgaagctcctgaaagaaggtaatttaacgtctattttccatccactggtttttcattggttcaaaaattgtgtgggcacatctcataaatatttcttcatggtttataggtttagtggaacaattattggtgttgggagcatgtcaacgtctccatgggcaaattctgactggagatctttgaaggtaattaagattttcaaatttgtgggcaggatgcccttcttcctctgcatctgctttctggaacatttgaggacatatgtttcttagaaaatgcctgcagttgtgttgtcatgttttctcttgtatatcttccagaatcacaacccatgtgttcaccactgataggtccaatgggatgagccttctgtggttcctcgtccagatagagtttcaccatgggaactagagccacttgctgtgagtaattcacagccatcacctcagccaccggcaagaaataagcgggctcggcctcctgcttcgaattcaattgctccagaactacctccggtcttcggtatgtttttactaagatattgctacagattgtgcttctgctttaaggtgatagctagtactgttttagcaatagaaaccttgcagtgtactgcttgtctgtctcttcaattgctgtctgctttgcatgtctaacctcttctgttcctaacttaggtttgtggaaatcttcagctgagtccacacaaggcttctcattttcgggactacagcgaacacaggaactatatccttcaagccccaatccgatcttctcaacatcattaaatgttggattcagtacaaagaatgagccatctgctctaagcaacaagcatttttactggccaatgagagagacaagagccaactcttactctgctagcatcagcaaagttccatctgaaaagaagcaagagcctagttcggctggctgcagattgtttggaatcgagataagctctgctgtggaagctacatctccactggctgctgtttctggtgttggtcaagaccaaccagctgcctcagtagatgctgagtctgatcagctctcacaaccatcacatgccaacaaatctgatgctccagcggcaagcagtgagccatctcctcatgagactcagagtcggcaagtgaggagctgcaccaaggtaatgagggttatttttgcttgcaatactcaatatcctcttgtggacagcggagtaattaataattggtcttcttgcaggtcatcatgcaaggaatggcagttggaagggcagtggacctgacaaggctccatggatatgatgatcttcgctgcaagttagaagagatgtttgatatccaaggagagctctctgctagcctaaagaaatggaaggttgtttacacagatgatgaggatgatatgatgctggttggggatgacccttggccgtatgtacccttatccctgttcaattaattacttcattttttgaattccttgagccttaactaattacatttcgattgttgccactgcagtgaattttgcagcatggtgaaaaggatatacatctacacctacgaggaagccaagcaacttactcccaagtcgaagctgccgatcattggcgacgccatcaagccaaacccaaacaaacaatcgcccgagtctgacatgcctcacagtgacctggacagcaccgcccccgtcaccgataaggattgctgaatgctacctggccatatttcccttcagttttttttttccatcttgggatgttgagcatgggggccaatgcccatgatccttgggacttctaagagttatccgctggttggttcgtgggcccgaaggacaccgtctgcatctaccatggttcatgcatcatgcatagctgcccctggaccagtgtggatcccatgccaggaaggatggctggggaggcagcatttccatcacctggcattccctgcgtgattggcccatccgtgtttaattctgctgtgtgggggtagtaggagccatggcatcctttgtccagtgctcagttttggtaaagtggtgactggtggtagacctgcaagaacggggcccgggcctcgccatctctcgctgcaccagtaaatagagtgtcaagcattcatgttcctttcttcctgcaatgcatggggtatcatcatccgtagctaagcatcagttcttctccccccccccccccccccttgcatcctgttcgcagttccggtgccgccactggagctgttcgttttaacccaccggcattccttcttttctttttttttaccgggtggtgtttttgctttcggaagtcggagcatttgctaagtttttttttcctacgctgtgctttcctcctgctgaatgtacctgtgcttttcctcctcgtggaagccatccaaatctatgacaattttctgtactcacttacctgcatgtacataggaatggaaatggaactggactgaactgaacctg</dnaseqindica>
External Link(s)	NCBI Gene:Os02g0557200, RefSeq:Os02g0557200